Farklı Dozlarda Putresin ve Indol–3–Bütirik Asidin Birlikte Uygulamalarının Cabernet Franc/5BB Aşı Kombinasyonunda Kallus Gelişimi Üzerine Etkileri

Öz

Bu araştırma, 2013 yılında Namık Kemal Üniversitesi Ziraat Fakültesi Bahçe Bitkileri Bölümüne ait olan aşı ünitesinde gerçekleştirilmiştir. Çalışmada, putresin (PUT; 0, 2.5, 5 ve 10 mM) ve indol–3–asetik asidin (IBA; 0 ppm (–) ve 2000 ppm (+)) birlikte uygulanan farklı dozlarının Cabernet Franc/5BB aşı kombinasyonunda kallus oluşumu üzerine etkileri incelenmiştir. Deneme sonucunda, Cabernet Franc/5BB aşı kombinasyonunda putresinin artan dozları olumlu sonuçlar vermekle birlikte, en iyi sonuçlar indol–3–asetik asit ilaveli uygulamalardan elde edilmiştir. Uygulama dozları arasında özellikle PUT 10 mM+IBA (+) uygulamasının Cabernet Franc/5BB aşı kombinasyonunda incelenen parametreler açısından en iyi sonuçları verdiği görülmüştür.

Anahtar Kelimeler

• Vitis vinifera L.
• omega aşısı
• putresin
• indol–3–bütirik asit
• kallus oluşumu

Influences of Various Doses of Putrescine Applications With Indole–3–Butyric Acid on Callus Development in Grafting Combination of Cabernet Franc/5BB

Abstract

This research was carried out at grafting unit of Namık Kemal University, Agriculture Faculty, Department of Horticulture in 2013 year. In study, influences of putrescine doses (PUT; 0, 2.5, 5 and 10 mM) with or without indole–3–acetic acid (IBA; 0 ppm (–) and 2000 ppm (+)) on callus formation of Cabernet Franc/5BB grafting combination were examined. However, increasing doses of putrescine applications caused affirmative results, best results were obtained from applications of indole–3–acetic acid added putrescine at the end of experiment. Among the application doses, it was seen that PUT 10 mM+IBA (+) application especially caused best results in terms of examined parameters in Cabernet Franc/5BB grafting combination.

Keywords

• Vitis vinifera L.
• omega grafting
• putrescine
• indole–3–butyric acid
• callusing

Kaynakça

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