Euphorbia rigida Yaprak Ekstraktından Gümüş Nanopartiküllerin Sentezi, Optimizasyonu ve Karakterizayonu ve Antimikrobiyal Potansiyellerinin Araştırılması

Abstract

Yeşil sentez; çevre dostu ve maliyet açısından ucuz olması, kimyasal ve fiziksel yöntemlerden daha çok tercih edilen nanopartiküllerin kolay bir şekilde elde edilmesi ile bilinen biyolojik bir yöntemdir. Potansiyel biyolojik aktiviteye sahip çeşitli metallik nanopartiküller bitki özütleri kullanılarak sentezlenmektedir. Bu çalışmada, sütleğengil (Euphorbia rigida) yaprak sulu özütü kullanılarak, yeşil sentez yöntemi ile gümüş nanopartikülleri (AgNP) sentezlendi. Sentezlenen nanpartikülerin antimikrobiyal aktiviteleri mikrodilüsyon yöntemi ile belirlendi.Yeşil sentezin optimizasyonu, sıcaklık, pH, sentez zamanı, AgNO3 ve bitki özütünün farklı konsantrasyonları gibi farklı parametrelerin denenmesi ile gerçekleştirildi. Euphorbia rigida yaprak özütünden
sentezlenen gümüş nanopartiküller (Er-AgNPs), Ultraviyole/görünür ışık absorbsiyon spektrofotometresi (Uvvis), Dinamik ışık saçılımı (DLS), Taramalı Elektron Mikroskobu (SEM) analizleri ile karakterize edildi. Altı farklı patojene karşı antimikrobiyal aktiviteleri belirlendi. Bu çalışmada, Euphorbia rigida bitkisinin yaprak özütüne ait örneğin parçacık boyutunun histogramının 50 ile 75 nm olan küresel yapılı Ag nanopartiküller sentezlendi. Er-AgNPs’ler çeşitli patojenlere (Escherichia coli, Staphylococcus aureus, Enterococcus faecium, Staphylococcus epidermidis, Salmonella enteritidis, Salmonella typhimurium, Listeria monocytogenes and Candida albicans) karşı güçlü bir antimikrobiyal aktivite gösterdi. Sonuç olarak, sentezlenen Er-AgNPs biyomedikal, farmasötik, biyoteknoloji ve endüstriyel alandaki uygulamalar için biyolojik bir materyal olarak kullanılabilir. Bu çalışmada, ilk kez Euphorbia rigida yaprak özütü kullanılarak hazırlanan gümüş nanopartiküllerin antimikrobiyal aktivitesi belirlendi.

Keywords

• Antimikrobiyal Aktivite
• Dinamik Işık Saçılımı
• Euporbia Rigida
• Taramalı Elektron Mikroskobu
• Gümüş Nanopartikül

Green Synthesis, Optimization, and Characterization of Silver Nanoparticles from Euphorbia rigida Leaf Extract and Investigation of Their Antimicrobal Activities

Abstract

Green synthesis is a biological method known for being environment-friendly, cheap and for retrieval of nanoparticles with ease, which is preferred more than chemical and physical methods. Various metallic nanoparticles with potent bioactivities have been synthesized using plant extracts. In this study, synthesis of silver nanoparticles (AgNPs) using the aqueous leaf extract of Euphorbia rigida was investigated. Their antibacterial activity was determined using microdilution methods. The optimization of the synthesis was performed via the adjustment of different parameters including pH, temperature, concentration of the plant extract, the different concentrations of AgNO3, and synthesis time using UVvisible spectroscopy. Silver nanoparticles (Er-AgNPs) that were synthesized by Euphorbia rigida characterized by Dynamic Light Scattering (DLS) and Scanning Electron Microscope (SEM) analyses and their antimicrobial potential were tested on six different pathogens. The synthesis process resulted in spherical-shaped NPs with a size ranging between 50-75nm. The AgNPs demonstrated a strong antimicrobial activity against various pathogens (Escherichia coli, Staphylococcus aureus, Enterococcus faecium, Staphylococcus epidermidis, Salmonella enteritidis, Salmonella typhimurium, Listeria monocytogenes and Candida albicans). The results showed that the synthesized Er-AgNPs can be used as a biological material for biomedical, pharmaceutical, biotechnology and industrial areas. In this study, the antimicrobial potential of silver nanoparticles which are prepared using Euphorbia rigida leaf extract isreported for the first time. 

Keywords

• Antimicrobial Activity
• Dynamic Light Scattering
• Euporbia Rigida
• Scanning Electron Microscope
• Silver Nanoparticle

Thanks

The scanning electron microscopy (SEM) measurements for surface morphology of nanoparticles were performed in the Ege University Application and Research Center for Testing and Analysis (EGE-MATAL). I also thank Associate professor Hasan YILDIRIM for kindly providing the plant.

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