Alüminyuma Maruz Kalan Sıçanların Böbreklerinde 3-Benzoil-7-Hidroksi Kumarin Bileşiğinin TNF-α ve Kaspaz-3 İmmün Reaktivitesi Üzerine Etkileri

Abstract

Bu çalışmada, Alüminyum (Alu) ile tedavi edilen sıçanların böbrek dokularında 3-benzoil-7-hidroksi kumarin bileşiğinin apoptotik belirteçler üzerindeki etkilerini araştırmayı amaçladık. Deney için 28 adet Wistar albino erkek ratlar 4 gruba (n=7) ayrıldı; Kontrol Grubu: Deney süresince sıçanlara gün aşırı 1 ml/kg %0.9 NaCl oral gavaj yolu ile verildi. Alüminyum (Alu) Grubu: Suda çözünmüş, Alu bileşiği intraperitonal(i.p.) yol ile 30 gün boyunca 8.3 mg/kg/gün aşırı uygulandı. Tedavi Grubu: Sıçanlara gün aşırı 10 mg/kg 3-Benzoyl-7-Hydroxy kumarin oral gavaj yolu ile 30 gün süreyle uygulandı. Alu + Tedavi Grubu: 8.3 mg/kg Alu su içinde hazırlandı ve i.p. deney süresi boyunca gün aşırı 10 mg/kg 3-Benzoyl-7-Hydroxy kumarin oral gavaj ile uygulandı. 30 günlük deney sonunda anestezi altında hayvanların böbrek dokusu alınarak uygun koşullarda saklandı. Elde edilen verilere göre Alu uygulaması böbrek dokusunda Kaspaz-3, TNF-α ve MDA düzeylerinin artmasına neden olmuş ve 3-benzoil-7-hidroksi kumarin bileşiğinin bu değişiklikleri önemli ölçüde tersine çevirdiği görüldü.

Keywords

• Oksidatif stres
• Apoptoz
• Proinflamatuar sitokin
• İmmünohistokimya

Effects of 3-Benzoyl-7-Hydroxy Coumarin Compound on TNF-α and Caspase-3 Immunreactivity in Kidneys of Rats Exposed to Aluminum

Abstract

In this study, we aimed to investigate the effects of 3-benzoyl-7-hydroxy coumarin compound on apoptotic markers in the kidney tissues of rats treated with Aluminum(Alu). 28 Wistar albino male rats were divided into four groups (n = 7) as: Control Group: 1 ml/kg 0.9% NaCl was administered by oral gavage every other day during the experiment. Aluminium (Alu) Group: Alu was prepared in water and given by intraperitoneal(i.p) for 30 days at an excess of 8.3 mg/kg/day. Treatment Group: 10 mg/kg 3-Benzoyl-7-Hydroxy Coumarin was administered to rats by oral gavage every other day for 30 days. Alu + Treatment Group: 8.3 mg/kg Alu was prepared in water and administered i.p for 30 days every other day, and then 10 mg/kg 3-Benzoyl-7-Hydroxy Coumarin was administered to rats by oral gavage every other day for 30 days. At the end of the 30-day experiment, the kidney tissue of the animals were removed under anesthesia and stored under appropriate conditions. In according to our data, Alu administration caused increased Caspase-3 TNF-α and MDA levels in kidney tissue, and 3-benzoyl-7-hydroxy coumarin was found to significantly reverse these changes.

Keywords

• Oxidative stress
• Apoptosis
• Proinflamatuar cytokine
• Immunohistochemistry

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