Effects of 3-Benzoyl-7-Hydroxy Coumarin Compound on TNF-α and Caspase-3 Immunreactivity in Kidneys of Rats Exposed to Aluminum

Yıl 2023, , 73 - 78, 30.06.2023

Ahmet Türk , Tuncay Kuloğlu , Büşra Zencirci , Ali Gürel , Abdullah Karadağ , Yusuf Özay , Serhat Hançer , Muhammed Emre Yüzer , Kenan Türkan , Ahmet Özkaya

https://doi.org/10.31594/commagene.1217426

Cited By: 1

Öz

In this study, we aimed to investigate the effects of 3-benzoyl-7-hydroxy coumarin compound on apoptotic markers in the kidney tissues of rats treated with Aluminum(Alu). 28 Wistar albino male rats were divided into four groups (n = 7) as: Control Group: 1 ml/kg 0.9% NaCl was administered by oral gavage every other day during the experiment. Aluminium (Alu) Group: Alu was prepared in water and given by intraperitoneal(i.p) for 30 days at an excess of 8.3 mg/kg/day. Treatment Group: 10 mg/kg 3-Benzoyl-7-Hydroxy Coumarin was administered to rats by oral gavage every other day for 30 days. Alu + Treatment Group: 8.3 mg/kg Alu was prepared in water and administered i.p for 30 days every other day, and then 10 mg/kg 3-Benzoyl-7-Hydroxy Coumarin was administered to rats by oral gavage every other day for 30 days. At the end of the 30-day experiment, the kidney tissue of the animals were removed under anesthesia and stored under appropriate conditions. In according to our data, Alu administration caused increased Caspase-3 TNF-α and MDA levels in kidney tissue, and 3-benzoyl-7-hydroxy coumarin was found to significantly reverse these changes.

Anahtar Kelimeler

Oxidative stress, Apoptosis, Proinflamatuar cytokine, Immunohistochemistry

Destekleyen Kurum

None

Proje Numarası

None

Teşekkür

Special thanks to Prof. Dr. Murat KOCA (Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Adiyaman University) and Prof. Dr Adnan Kurt (Department of Chemistry, Faculty of Arts and Sciences, Adıyaman University) for their contribution to this study.

Alüminyuma Maruz Kalan Sıçanların Böbreklerinde 3-Benzoil-7-Hidroksi Kumarin Bileşiğinin TNF-α ve Kaspaz-3 İmmün Reaktivitesi Üzerine Etkileri

Öz

Bu çalışmada, Alüminyum (Alu) ile tedavi edilen sıçanların böbrek dokularında 3-benzoil-7-hidroksi kumarin bileşiğinin apoptotik belirteçler üzerindeki etkilerini araştırmayı amaçladık. Deney için 28 adet Wistar albino erkek ratlar 4 gruba (n=7) ayrıldı; Kontrol Grubu: Deney süresince sıçanlara gün aşırı 1 ml/kg %0.9 NaCl oral gavaj yolu ile verildi. Alüminyum (Alu) Grubu: Suda çözünmüş, Alu bileşiği intraperitonal(i.p.) yol ile 30 gün boyunca 8.3 mg/kg/gün aşırı uygulandı. Tedavi Grubu: Sıçanlara gün aşırı 10 mg/kg 3-Benzoyl-7-Hydroxy kumarin oral gavaj yolu ile 30 gün süreyle uygulandı. Alu + Tedavi Grubu: 8.3 mg/kg Alu su içinde hazırlandı ve i.p. deney süresi boyunca gün aşırı 10 mg/kg 3-Benzoyl-7-Hydroxy kumarin oral gavaj ile uygulandı. 30 günlük deney sonunda anestezi altında hayvanların böbrek dokusu alınarak uygun koşullarda saklandı. Elde edilen verilere göre Alu uygulaması böbrek dokusunda Kaspaz-3, TNF-α ve MDA düzeylerinin artmasına neden olmuş ve 3-benzoil-7-hidroksi kumarin bileşiğinin bu değişiklikleri önemli ölçüde tersine çevirdiği görüldü.

Anahtar Kelimeler

Oksidatif stres, Apoptoz, Proinflamatuar sitokin, İmmünohistokimya

Proje Numarası

None

Kaynakça

• Abdel Moneim, A.E., Othman, M.S., Mohmoud, S.M., & El-Deib, K.M. (2013). Pomegranate peel attenuates aluminum-induced toxicity mechanisms and methods, Hepatorenal Toxicity, 23(8), 624-633 https://doi.org/10.3109/15376516.2013.823634
• Al-Kahtani, M., Abdel-Daim, M.M., Sayed, A.A., El-Kott, A., & Morsy, K. (2020). Curcumin phytosome modulates aluminum-induced hepatotoxicity via regulation of antioxidant, Bcl-2, and caspase-3 in rats. Environmental Science and Pollution Research, 27(17), 21977-21985. https://doi.org/10.1007/s11356-020-08636-0
• Al-Qhtani, S. A., & Farran, S. K. (2017). The protective and therapeutic effect of resveratrol in improving renal and hepatic failure induced by aluminum chloride in experimental animals. Journal of American Science, 13(10), 26. https://doi.org/10.7537/marsjas131017.03 Atmaca, M., Bilgin, H.M., Obay, B.D., Diken, H., Kelle, M., & Kale, E. (2011). The hepatoprotective effect of coumarin and coumarin derivates on carbon tetrachloride-induced hepatic injury by antioxidative activities in rats. Journal of Physiology and Biochemistry, 67(4), 569-576. https://doi.org/10.1007/s13105-011-0103-5
• Baud, V., & Karin, M. (2001). Signal transduction by tumor necrosis factor and its relatives. Trends in Cell Biology, 11(9), 372-377. https://doi.org/10.1016/s0962-8924(01)02064-5
• Campbell, A. (2004). Inflammation, neurodegenerative diseases, and environmental exposures. Annals of the New York Academy of Sciences, 1035(1), 117-132. https://doi.org/10.1196/annals.1332.008
• Chang, W.S., Lin, C.C., Chuang, S.C., & Chiang, H.C. (1996). Superoxide anion scavenging effect of coumarins. The American journal of Chinese medicine, 24(1), 11-17. https://doi.org/10.1142/s0192415x96000037
• El-Demerdash, F.M., Baghdadi, H.H., Ghanem, N.F., & Mhanna, A.B.A. (2020). Nephroprotective role of bromelain against oxidative injury induced by aluminium in rats. Environmental toxicology and pharmacology, 80, 103509. https://doi.org/10.1016/j.etap.2020.103509
• Emmanuel‐Giota, A.A., Fylaktakidou, K.C., Litinas, K.E., Nicolaides, D.N., & Hadjipavlou‐Litina, D.J. (2001). Synthesis and biological evaluation of several 3‐(coumarin‐4‐yl) tetrahydroisoxazole and 3‐(coumarin‐4‐yl) dihydropyrazole derivatives. Journal of Heterocyclic Chemistry, 38(3), 717-722. https://doi.org/10.1002/jhet.5570380329
• Eser, N., Yoldas, A., Turk, A., Kalaycı Yigin, A., Yalcin, A., & Cicek, M. (2021). Ameliorative effects of garlic oil on FNDC5 and irisin sensitivity in liver of streptozotocin-induced diabetic rats. Journal of Pharmacy and Pharmacology, 73(6), 824-834. https://doi.org/10.1093/jpp/rgab023
• Exley, C. (2004). The pro-oxidant activity of aluminum. Free Radical Biology and Medicine, 36(3), 380-387. https://doi.org/10.1016/j.freeradbiomed.2003.11.017
• Güleş, Ö., & Ülker, E. (2008). Apoptozun belirlenmesinde kullanılan yöntemler. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 19(2), 73-78. ISSN 1017-8422; e-ISSN: 1308-3651
• Gürel, A., Türk, A., Aydın, H., Genç, F., & Yalçın, A. (2022). Cinnamon's effect on apoptosis, inflammation and trpm2 channels in methotrexate induced renal damage. International Journal of Advanced Research in Medicine, 4(2), 06-10. https://doi.org/10.22271/27069567.2022.v4.i2a.387
• Hasona, N.A., & Ahmed, M.Q. (2017). Antioxidant and ameliorative effects of Zingiber officinale against aluminum chloride toxicity. International Journal of Chinese Medicine 1(4), 124-131.
• Ichikawa, I., Kiyama, S., & Yoshioka, T. (1994). Renal antioxidant enzymes: their regulation and function. Kidney International, 45(1), 1-9. https://doi.org/10.1038/ki.1994.1
• Idriss, H. T., & Naismith, J.H. (2000). TNFα and the TNF receptor superfamily: Structure‐function relationship (s). Microscopy Research and Technique, 50(3), 184-195. https://doi.org/10.1002/1097-0029(20000801)50:3<184::AID-JEMT2>3.0.CO;2-H
• Kaya, S., Yalçın, T., Boydak, M., & Dönmez, H.H. (2022). Protective Effect of N-Acetylcysteine Against Aluminum-Induced Kidney Tissue Damage in Rats. Biological Trace Element Research, 1-10. https://doi.org/10.1007/s12011-022-03276-6
• Khan, N., Sharma, S., & Sultana, S. (2004). Amelioration of ferric nitrilotriacetate (Fe-NTA) induced renal oxidative stress and tumor promotion response by coumarin (1, 2-benzopyrone) in Wistar rats. Cancer Letters, 210(1), 17-26. https://doi.org/10.1016/j.canlet.2004.01.011
• Kurt, A., Ayhan, A. F., & Koca, M. (2018). Kumarin Türevli Kopolimerlerin Sentezi ve Karakterizasyonu. Sakarya University Journal of Science, 22(3), 880-887. https://doi.org/10.16984/saufenbilder.322354
• Kurt, A., Gündüz, B., & Koca, M. (2019). A detailed study on the optical properties of 3-benzoyl-7-hydroxy coumarin compound in different solvents and concentrations. Macedonian Journal of Chemistry and Chemical Engineering, 38(2), 227-236. https://doi.org/10.20450/mjcce.2019.1403
• Kurt, A., & Topsoy, O. (2017). Preparation of novel coumarin cyclic polymer/montmorillonite based nanocomposites. Russian Journal of Applied Chemistry, 90(12), 2019-2027. https://doi.org/10.1134/S1070427217120199
• Lentini, P., Zanoli, L., Granata, A., Signorelli, S.S., Castellino, P., & Dell'Aquila, R. (2017). Kidney and heavy metals-The role of environmental exposure. Molecular Medicine Reports, 15(5), 3413-3419. https://doi.org/10.3892/mmr.2017.6389
• Lien, E. J., Ren, S., Bui, H.H., & Wang, R. (1999). Quantitative structure-activity relationship analysis of phenolic antioxidants. Free Radical Biology and Medicine, 26(3-4), 285-294. https://doi.org/10.1016/S0891-5849(98)00190-7
• Liu, J., Wang, Q., Sun, X., Yang, X., Zhuang, C., Xu, F.,…..& Li, Y. (2016). The toxicity of aluminum chloride on kidney of rats. Biological Trace Element Research, 173(2), 339-344. https://doi.org/10.1007/s12011-016-0648-9
• Marshall, F.F. (2005). Attenuation of potassium bromate-induced nephrotoxicity by coumarin (1, 2-benzopyrone) in Wistar rats: chemoprevention against free radical-mediated renal oxidative stress and tumor promotion response. The Journal of Urology, 174(6), 2145-2145. https://doi.org/10.1179/135100004225003860
• McIlwain, D. R., Berger, T., & Mak, T. W. (2013). Caspase functions in cell death and disease. Cold Spring Harbor perspectives in Biology, 5(4), a008656. https://doi.org/10.1101/cshperspect.a008656
• Nicholson, D.W. (1999). Caspase structure, proteolytic substrates, and function during apoptotic cell death. Cell Death Differ, 6(11), 1028-1042. https://doi.org/10.1038/sj.cdd.4400598
• Nofal, Z., El-Zahar, M., & Abd El-Karim, S. (2000). Novel coumarin derivatives with expected biological activity. Molecules, 5(2), 99-113. https://doi.org/10.3390/50200099
• Othman, M.S., Fareid, M.A., Abdel Hameed, R.S., & Abdel Moneim, A.E. (2020). The protective effects of melatonin on aluminum-induced hepatotoxicity and nephrotoxicity in rats. Oxidative Medicine and Cellular Longevity 2020, 7375136, https://doi.org/10.1155/2020/7375136
• Ozkaya, A., & Türkan, K. (2021). Protective Effects of 3-benzoyl-7-hydroxy Coumarin on Liver of Adult Rat Exposed to Aluminium Chloride. Acta Chimica Slovenica, 68(1), 222-228.
• Palma, H.E., Wolkmer, P., Gallio, M., Corrêa, M.M., Schmatz, R., Thomé, G.R.., & Mazzanti, C.M. (2014). Oxidative stress parameters in blood, liver, and kidney of diabetic rats treated with curcumin and/or insulin. Molecular and Cellular Biochemistry, 386(1-2), 199–210. https://doi.org/10.1007/s11010-013-1858-5
• Patonay, T., Litkei, G., Bognár, R., & Miszti, C. (1984). Synthesis, antibacterial and antifungal activity of 4-hydroxycoumarin derivatives, analogues of novobiocin. Pharmazie, 39(2), 84-91.
• Placer, Z.A., Cushman, LL., & Johnson, B.C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry, 16(2), 359-364. https://doi.org/10.1016/0003-2697(66)90167-9
• Rondeau, V., Commenges, D., Jacqmin-Gadda, H., & Dartigues, J.-F. (2000). Relation between aluminum concentrations in drinking water and Alzheimer's disease: an 8-year follow-up study. American Journal of Epidemiology, 152(1), 59-66. https://doi.org/10.1093/aje/152.1.59
• Sun, X., Cao, Z., Zhang, Q., Liu, S., Xu, F., Che, J.,..…. &. Liang, W. (2015). Aluminum trichloride impairs bone and downregulates Wnt/β-catenin signaling pathway in young growing rats. Food and Chemical Toxicology, 86, 154-162. https://doi.org/10.1016/j.fct.2015.10.005
• Tichati, L., Benzaid, C., Trea, F., Mahmoud, R., & Kheireddine, O. (2022). Ameliorating effects of Rhamnus alaternus L. aqueous extract on aluminum chloride-induced nephrotoxicity via attenuation of oxidative stress in male Wistar rats. Comparative Clinical Pathology, 1-12. https://doi.org/10.1007/s00580-022-03405-6
• Turner, M.D., Nedjai, B., Hurst, T., & Pennington, D.J. (2014). Cytokines and chemokines: At the crossroads of cell signalling and inflammatory disease. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1843(11), 2563-2582. https://doi.org/10.1016/j.bbamcr.2014.05.014
• Ucer, O., & Kocaman, N. (2022). New candidates in the differential diagnosis of malignant mesothelioma from benign mesothelial hyperplasia and adenocarcinoma; DARS2 and suprabasin. Tissue and Cell, 79, 101920. https://doi.org/10.1016/j.tice.2022.101920
• Venugopala, K.N., Rashmi, V., & Odhav, B. (2013). Review on natural coumarin lead compounds for their pharmacological activity. BioMed Research International 2013, 963248. https://doi.org/10.1155/2013/963248
• Williams, R. J. (1999). What is wrong with aluminium? The J.D. Birchall Memorial Lecture. Journal of Inorganic Biochemistry, 76(2), 81-88. https://doi.org/10.1016/s0162-0134(99)00118-x
• Xu, F., Liu, Y., Zhao, H., Yu, K., Song, M., Zhu, Y., & Li, Y. (2017). Aluminum chloride caused liver dysfunction and mitochondrial energy metabolism disorder in rat. Journal of inorganic biochemistry, 174, 55-62. https://doi.org/10.1016/j.jinorgbio.2017.04.016
• Yousef, M. I., Mutar, T. F., & Kamel, M. A. E.-N. (2019). Hepato-renal toxicity of oral sub-chronic exposure to aluminum oxide and/or zinc oxide nanoparticles in rats. Toxicology Reports, 6, 336-346. https://doi.org/10.1016/j.toxrep.2019.04.003
• Xu, F., Ren, L., Song, M., Shao, B., Han, Y., Cao, Z., &, Li, Y. (2018). Fas-and mitochondria-mediated signaling pathway involved in osteoblast apoptosis induced by AlCl 3. Biological trace element research, 184, 173-85. https://doi.org/10.1007/s12011-017-1176-y
• Yu, Q., Zhu, K., Ding, Y., Han, R., Cheng, D. (2022). Comparative study of aluminum (Al) speciation on apoptosis-promoting process in PC12 cells: Correlations between morphological characteristics and mitochondrial kinetic disorder. Journal of Inorganic Biochemistry, 232, 111835. https://doi.org/10.1016/j.jinorgbio.2022.111835