Sarı Soğan (Allium Cepa) Kabuğu Ekstraktının Çiğ, UHT ve Pastörize Sütlere İnoküle Edilen Bazı Patojenler Üzerindeki Antimikrobiyal Etkisinin Belirlenmesi

Year 2022, , 707 - 716, 17.10.2022

Sabire Yerlikaya , Merve Çiftçi Aysun İşler Hülya Şen Arslan

https://doi.org/10.21605/cukurovaumfd.1190323

Cited By: 1

Abstract

Bu araştırmada sarı soğan kabuğu ekstraktının (YOPE), pastörize, UHT ve çiğ sütlere inoküle edilen bazı patojenler üzerindeki inhibitör etkisi incelenmiştir. Süt örneklerine %10 (v/v) YOPE ile ilave edilerek 4 ºC’de 5 gün inkübasyona bırakılmıştır. Süt örneklerinin pH ve rengi depolamanın 1, 3 ve 5. günlerinde belirlenmiştir. Ayrıca, süt numunelerine ayrı ayrı 0,0, 5,0 ve 7,0 log kob/mL konsantrasyonlarında E. coli ATCC 25922 ve S. aureus ATCC 25923 inoküle edilmiştir. Bahsedilen numunelerin tümüne ayrı ayrı %0,0 ve 5,0 (v/v) konsantrasyonunda YOPE ilave edilmiştir. YOPE en yüksek inhibitör etkiyi 7,00±0,24 log kob/mL konsantrasyonunda E. coli ile kontamine edilmiş pastörize sütte gösterirken; en düşük inhibitör etkileri 7,00±0,87 log kob/mL konsantrasyonunda S. aureus ile 5,00±0,21 log kob/mL konsantrasyonunda E.coli ile kontamine edilmiş çiğ sütte göstermiştir.

Keywords

Antimikrobial aktivite, Süt, E. coli, S. aureus

Determining Antibacterial Effect of Yellow Onion (Allium Cepa) Peel Extract on Some Pathogen Inoculated in Raw, UHT and Pasteurized Milks

Abstract

In this research, yellow onion peel extract (YOPE) was tested against some pathogens inoculated into pasteurized, UHT and raw milks. Milk samples were added with YOPE at 10% (v/v) and were left at 4 ºC for 5 days. The pH and colour of milk samples were determined at 1, 3 and 5th day of storage. Moreover, the milk samples were inoculated by 0.0, 5.0 and 7.0 log cfu/mL of Escherichia coli ATCC 25922 and by 0.0, 5.0 and 7.0 log cfu/mL of S. aureus ATCC 25923. All the above samples were then added with YOPE at a rate of 0.0 or 5.0% (v/v). YOPE had the greatest inhibitory effect on E. coli inoculated pasteurized milk at a concentration of 7.00±0.24 log cfu/mL, whereas the lowest inhibitory effect was shown on the raw milk inoculated with 7.00±0.87 log cfu/mL S. aureus and 5.00±0.21 log cfu/mL E. coli.

Keywords

Antibacterial activity, Milk, E. coli, S. aureus

References

• 1. Beykaya, M., Özbey, A., Yıldırım, Z., 2017. Determination of Physical, Chemical and Microbiological Properties of Milk From Some Dairy Plants in Sivas Province. Turkish Journal of Agriculture-Food Science and Technology 5(4), 388-396.
• 2. Şenel, E., 2017. Ayın Konusu: Süt. Türk Tarım Dergisi, 237, 16-17. 3. Muehlhoff, E., Bennett, A., McMahon, D., 2013. Milk and Dairy Products in Human Nutrition. Food and Agriculture Organization of the United Nations (FAO), Rome.
• 4. Özdemir, D., 2019. Determination of Some Microbiological, Physical and Chemical Quality of Milk Provided from Milk Collection Centers in Kastamonu. Master’s Thesis, Kastamonu University, Institute of Science, the Degree of Master of Philosophy in Food Engineering, Kastamonu, 93.
• 5. Dehinenet, G., Mekonnen, H., Ashenafi, M., Emmanuelle, G., 2013. Determinants of Raw Milk Quality Under a Smallholder Production System in Selected Areas of Amhara and Oromia National Regional States, Ethiopia. Agric. Biol. J. N. Am. 4(1), 84-90.
• 6. Metin, M., 2017. Süt Teknolojisi: Sütün Bileşimi ve İşlenmesi. 15. Baskı, E.Ü. Mühendislik Fakültesi Yayınları, İzmir, 793.
• 7. Sudhasaravanan, R., Binukumari, S., 2015. Microbial Quality of Raw and Pasteurized Milk Samples Collected from Different Regions of Madurai District, (T.N.) India. IOSR Journal of Environmental Science, Toxicology and Food Technology, 9(1), 71-73.
• 8. Yerlikaya, O., Kınık, Ö., 2019. Consumption of Raw Cow Milk and Heat Treated Drinking Milk in Terms of Food Safety and Human Health, Journal of Food and Feed Science- Technology 22, 1-10.
• 9. Kara, R., Acaröz, U., Gürler, Z., Soylu, A., Küçükkurt, O., 2019. Determination of the Presence of Escherichia coli O157 and Listeria monocytogenes in Fresh Lettuce Samples. European Journal of Science and Technology 16, 871-873.
• 10. Çubukçı, S., Aydemir Atasever, M., 2018. Microbial Quality of Ice Cream Sold By Retail Outlets in Erzurum. Atatürk University Journal of Veterinary Sciences, 13(1), 54-62.
• 11. Özçelik, H., 2019. Soğan (Allium cepa) ve Sarımsak (Allium sativum l.) Atıklarının Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Mantar Hastalığının Önlenmesinde Kullanılabilirliğinin Araştırılması. Master’s Thesis, Gaziosmanpaşa University, Institute of Science, Tokat, 76. 12. Ergüven, İ., 2019. Detection of Phytoplasmas in Onion (Allium cepa L.) Production Areas of Adana Province. Master’s thesis, Harran University, Institute of Science, Şanlıurfa, 40.
• 13. Albayrak, B., Elmacı, Ö.L., 2017. Efect of Nitrogen and Sulphur Applications on the Amount of Vitamin C, Antioxidant Activity and Total Phenolic Matter of Dry Onion (Allium cepa L). Journal of Adnan Menderes University Agricultural Faculty, 14(2),1-5.
• 14. Kim, W., Lee, K., Kim, K., Chung, M., Cho, S., Paik, H., 2011. Antimicrobial Effects of Onion (Allium cepa L.) Peel Extracts Produced Via Subcritical Water Extraction Against Bacillus cereus Strains as Compared with Ethanolic and Hot Water Extraction, Food Science and Biotechnology, 20.
• 15. Lee, K., Kim, K., Nah, S., Chung, M., Cho, S., Paik, H., 2011. Antimicrobial and Antioxidative Effects of Onion Peel Extracted by the Subcritical Water, Food Science and Biotechnology, 20, 543–548.
• 16. Kim, K., Yim, J., 2015. Antioxidative Activity of Onion Peel Extract in Obese Women: a Randomized, Double-blind, Placebo Controlled Study, J Cancer Prev. 20(3), 202–207.
• 17. Karaki, H., Maswadeh, I., Abu Khalaf, E., Awawdeh, I., Wadaah, S., Hamdan, M., 2020. Antioxidant and Antimicrobial Activities of Onion Peels Extract and Fortification of White Wheat Flour to Prevent Health Hazard, Biomedical Sciences and Public Health, 1, 1.
• 18. Naqvı, S., Irfan, A., Zahoor, A., Zafar, M., Maria, A., Chand, A., Ashfaq, S., 2020. Determination of Antimicrobial and Antioxidant Potential of Agro-waste Peels, An. Acad. Bras. Ciênc. 92.
• 19. Hunt, M.C., Acton, J.C., Benedict, R.C., Calkins, C.R., Cornforth, D.P., Jeremiah, L.E., Olson, D.G., Salm, C.P., Savell, J.W., Shivas, S.D., 1991. Guidelines for Meat Color Evaluation, 44th Annual Reciprocal Meat Conference, Chicago, 9-12.
• 20. Lambooij, E., Potgieter, C.M., Britz, C.M., Nortje, G.L., Pieterse, C., 1999. Effect of Electrical and Mechanical Stunning Methods on Meat Quality in Ostriches. Meat Sci. 52, 331-337.
• 21. Lakade, A.J., Sundar, K., Shetty, P.H., 2017. Nanomaterial-based Sensor for the Detection of Milk Spoilage. LWT-Food Science and Technology, 75, 702-709.
• 22. Codina-Torrella, I., Guamis, B., Zamora, A., Quevedo, J.M., Trujillo, A.J., 2018. Microbiological Stabilization of Tiger Nuts’milk Beverage Usingultra-high Pressure Homogenization. A Preliminary Study on Microbialshelf-life Extension. Food Microbiology, 69, 143-150.
• 23. Metin, M., Öztürk, G., 2012. Süt ve Mamulleri Analiz Yöntemleri. Ege Üniversitesi Basımevi, Ege Üniversitesi Yayınları, Rektörlük Yayın No: 9. İzmir.
• 24. Akarca, G., Kahraman, A., Tomar, O., 2015. Change of the Shelf Life of Pasteurized Milk Which Added in Various Amonds of Cinnamon. Afyon Kocatepe University, Journal of Science and Engineering, 15, 1-9.
• 25. Diler, A., Baran, A., 2014. Determination of Some Quality Characteristics of Raw Cow Milk on Smallholder Dairy Farms in Hinis District of Erzurum. Alınteri Zirai Bilimler Dergisi, 26, 18-24.
• 26. Akın, M.S., Yapık, Ö., Akın, M.B., 2016. Some Properties of Raw Milk Obtained from Dairy Production Farm and Collectors in Adıyaman. Harran Journal of Agricultural and Food Sciences, 20, 253- 265.
• 27. Tuncer, K., Kul, E., Şahin, A., 2017. Determination of Some Quality Characteristics of Raw Milk Samples Collected from Dairy Farms in TR71 Region. Mediterranean Agricultural Sciences, 30(1), 65-69
• 28. Ateş, R., 2015. A Research: Determination of Changes in the Composition of Raw Milk Provided from Private Farms and Association of Manufacturers in Different Region of Turkey All the Year Round. Master’s Thesis, Namık Kemal University, Institute of Science, Tekirdağ, 41.
• 29. Anonim, 2009. Türk Gıda Kodeksi Çiğ Süt ve Isıl İşlem Görmüş İçme Sütleri Tebliği. Tebliğ No: 2009/14.
• 30. Yapık, Ö., 2014. A Comparative Study on the Determination of Some Chemical and Microbiological Properties of Street Milk and Raw Milk Produced in Milk Farm in Adıyaman. Master’s Thesis, Harran University, Institute of Science, Şanlıurfa, 59.
• 31. Çalışkan, Ş., 2019. The Impact of Hygiene Training on Raw Milk Quality: Sindirgi District Sample. Master’s Thesis, Celal Bayar University, Institute of Science, Manisa, 132.
• 32. Singh, N., Singh, V., Punia, P., 2019. Antibacterial Finish on Silk Fabric with Pomegranate and Onion Peel Extracts, Journal of Pharmacognosy and Phytochemistry, 5, 129- 131.
• 33. İlkimen, H., Gülbandılar, A., 2018. Investigation of Antimicrobial Effects of Lavender, Sage Tea, Thyme and Chamomile. Turkish Microbiological Society, 48(4), 241- 246
• 34. Serpi, M., Özdemir, Z.Ö., Salman, Y., 2012. Investigation of the Antibacterial Effects of Some Plant Extracts On Propionibacterium Acnes. KSÜ Journal of Agriculture and Nature, 15(1), 7-12.
• 35. Haşimi, N., Kızıl, S., Tolan, V., 2015. An Investigation on Antimicrobial Activities of Fennel and Sage Essential Oils. Batman University Journal of Life Sciences, 5(2), 227- 35.
• 36. Harlita, T.D., Oedjijono, Asnani, A., 2018. The Antibacterial Activity of Dayak Onion (Eleutherine palmifolia (L.) Merr) Towards Pathogenic Bacteria. Tropical Life Sciences Research, 29(2), 39–52.
• 37. Abdel-Rahim, A.M., Abdel-Malik, O.A., Idris, Sulieman, A.E. 2012. Antimicrobial Activity of the Extract of Garlic and Onions. Gezira Journal of Engineering and Applied Sciences, 7(1).
• 38. Al-Delamy, K.S., Ali, S.H., 2006. Effect of Onions Extracts on Staphylococcus aureus. Journal of the Science of Food and Agriculture, 21(2),110-112.