The effect of astaxanthin on amiodarone induced cardiac tissue damage in rat

Year 2023, Volume: 48 Issue: 1, 194 - 199, 31.03.2023

Özlem Kara Asuman Kilitci

https://doi.org/10.17826/cumj.1224847

Abstract

Purpose: The aim of this study was to evaluate the effect of astaxanthin on amiodarone induced cardiac tissue damage.
Materials and Methods: 3 groups were formed using 30 Wistar albino rats. In group 1 (control group) (n=10), neither any drugs were given nor anything was performed. In group 2 (amiodarone group) (n=10), 100 mg/kg amiodarone was given for 7 days. In group 3 (amiodarone+astaxanthin group) (n=10), 100 mg/kg amiodarone and 25 mg/kg astaxanthin were given for 7 days. Hearts were surgically extirpated in all groups. Blood malondialdehyde (MDA) levels and activities of catalase (CAT) and superoxide dismutase (SOD) were measured. Also, toxicity markers such as edema, hemorrhage, fibrosis, inflammatory cell infiltration were assessed by examining the slides prepared from cardiac tissue with microscopy.
Results: The MDA levels were significantly higher and the activities of SOD, and CAT were lower in group 2 than group 3. Tissue damage was significantly higher in group 2 than group 3.
Conclusion: According to our short term findings, astaxanthin reversed the toxicity of amiodarone on cardiac tissue. In the light of these promising results, we suggest that astaxanthin usage should be thought to protect the cardiac damage due to amiodarone.

Keywords

Amiodarone, astaxanthin, rat, cardiac tissue, toxicity

Astaksantinin sıçanda amiodarona bağlı kardiyak doku hasarı üzerine etkisi

Abstract

Amaç: BU çalışmada astaksantinin amiodaron kaynaklı kalp dokusu hasarı üzerindeki etkisinin değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntem: 30 adet Wistar albino rat kullanılarak 3 grup oluşturuldu. Grup 1'de (kontrol grubu) (n=10) herhangi bir ilaç verilmedi ve herhangi bir işlem yapılmadı. Grup 2'de (amiodaron grubu) (n=10) 7 gün boyunca 100 mg/kg amiodaron verildi. Grup 3'te (amiodaron+astaksantin grubu) (n=10) 7 gün boyunca 100 mg/kg amiodaron ve 25 mg/kg astaksantin verildi. Kalp tüm gruplarda cerrahi olarak ekstirpe edildi. Kan malondialdehid (MDA) seviyeleri ve katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri ölçüldü. Ayrıca kalp dokusundan hazırlanan preparatlar mikroskopla incelenerek ödem, hemoraji, fibrozis, inflamatuvar hücre infiltrasyonu gibi toksisite belirteçleri değerlendirildi.
Bulgular: Grup 2'de grup 3'e göre MDA düzeyleri anlamlı olarak yüksek, SOD ve CAT aktiviteleri daha düşüktü. Doku hasarı grup 2'de grup 3'e göre anlamlı olarak daha yüksekti.
Sonuç: Kısa dönem bulgularımıza göre astaksantin, amiodaronun kalp dokusu üzerindeki toksisitesini tersine çevirdi. Bu ümit verici sonuçların ışığında, biz amiodarona bağlı kardiyak hasardan korunmak için astaksantin kullanılmasının düşünülmesini öneriyoruz.

Keywords

Amiodaron, astaksantin, sıçan, kalp dokusu, toksisite

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