Balık sosu üretiminde kullanılan Lactobacillus sakei ve Staphylococcus carnosus bakterilerinin antioksidan aktivite kapasitesine etkisi
Year 2025,
Volume: 8 Issue: 1, 1 - 7, 30.06.2025
Fatma Çolakoğlu
,
Selin Özge Dinç
,
Fatma Nur Ergün
Abstract
Balık sosu fermantasyon tekniği ile üretilen, nitelikli fonksiyonel bir üründür. Kehribar renginde lezzetli bir sıvı olan ürünün yapısal özellikleri ortamda bulunan mikroorganizma ve enzim faaliyetleri ile şekillenmektedir. Yapılan bu çalışmada, hamsiden (Engraulis encrasicolus) geleneksel yöntem ve starter kültürle; Lactobacillus sakei ve Staphylococcus carnosus (BFL-F06, Almanya), balık sosu üretimi gerçekleştirilmiştir. Starter kullanımı, üründe geleneksel üretimden farklı olarak hem tuz oranın düşük olmasını hem de lezzet gelişiminin iyileştirilmesini vaat eden bir üretim şeklidir. Çalışmada, geleneksel üretimde % 20 oranında, starter ilaveli üretimde ise % 3 oranında tuz kullanılmış, sonuçta elde edilen ürün grupları, kalite özellikleri açısından da değerlendirilmiştir. Sos gruplarında sırasıyla; ham protein oranı % 10,72-12,43, ham yağ içeriği ise % 3,21-2,17 olarak belirlenmiştir. Duyusal açıdan, starter grubunda ekşi tadın, geleneksel sos grubunda ise tuzlu ve balıksı tadın baskın olduğu belirlenmiştir. DPPH (2,2-diphenyl-1-picrylhydrazyl) temizleme aktivitesi açısından ise, geleneksel üretimle elde edilen sos grubunda daha güçlü antioksidan aktivite belirlenmiştir.
Supporting Institution
TÜBİTAK
Project Number
1919B012222851
Thanks
Bu çalışmaya desteklerinden dolayı TÜBİTAK’a teşekkürlerimizi sunarız. Bu çalışma 2209-A 2022 yılı 2. dönem çağrısı kapsamında 1919B012222851 no’lu proje ile desteklenmiştir.
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Bu Y, Liu Y, Luan H, Zhu W, Li X, Li J. 2021. Changes in protease activity during fermentation of fish sauce and the correlation with antioxidant activity. J. Sci. Food Agric. 102(8):3150-3159
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Duan S, Hu X, Li M, Miao J, Du J, Wu R. 2016. Composition and metabolic activities of the bacterial community in shrimp sauce at the flavor-forming stage of fermentation as revealed by metatranscriptome and 16S rRNA gene sequencings. J. Agric. Food Chem. 64(12):2591-2603
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Elegado FB, Colegio SMT, Lim VMT, Gervasio ATR, Perez MTM, Balolong MP, Banaay CBG, Mendoza BC. 2016. Ethnic fermented foods of the Philippines with reference to lactic acid bacteria and yeasts. In: Tamang JP (ed) Ethnic fermented foods and alcoholic beverages of Asia. Springer, New York
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Feng Y, Su G, Zhao H, Cai Y, Cui C, Sun-Waterhouse D, Zhao M. 2015. Characterisation of aroma profiles of commercial soy sauce by odour activity value and omission test. Food Chem. 167: 220-228
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Hamzeh A, Noisa P, Yongsawatdigul J. 2020. Characterization of the antioxidant and ACE-inhibitory activities of Thai fish sauce at different stages of fermentation. J. Funct. Foods. 64:103699.
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Harrigan WF, McCance ME. 1976. Laboratory methods, 2nd edn. Academic Press, London
-
Je JY, Park PJ, Byun HG, Jung WK, Kim SK. 2005. Angiotensin I converting enzyme (ACE) inhibitory peptide derived from the sauce of fermented blue mussel, Mytilus edulis. Bioresour. Technol. 96(14):1624-1629
-
Lee SH, Jung JY, Jeon CO. 2015. Bacterial community dynamics and metabolite changes in myeolchi-aekjeot, a Korean traditional fermented fish sauce, during fermentation. Int. J. Food Microbiol. 203:15-22
-
Lee YC, Kung HF, Huang CY, Huang TC, Tsai YH. 2016. Reduction of histamine and biogenic amines during salted fish fermentation by Bacillus polymyxa as a starter culture. J Food Drug Anal. Journal List. 24(1):157-163
-
Ludorf W, Meyer V. 1973. Fische und fisherzeugnisse. In: Auflage Z, Parey PY (eds). Berlin und Hamburg
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Mueda RT. 2015. Physico-chemical and color characteristics of salt fermented fish sauce from anchovy stolephorus commersonii. AACL Bioflux. 8:565-572
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O’Bryan CA, Crandall PG, Ricke SC, Ndahetuye JB. 2015. Lactic acid bacteria (LAB) as antimicrobials in food products: types and mechanisms of action. In: Taylor TM (ed) Handbook of natural antimicrobials for food safety and quality, Woodhead Publishing, USA
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Ohata M, Uchida S, Zhou L, Arihara K. 2016. Antioxidant activity of fermented meat sauce and isolation of an associated antioxidant peptide. Food Chem. 194:1034-1039
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Ormancı HB, Künili İE, Çolakoğlu FA. 2018. Alabalık (Oncorhynchus mykiss) ve atıklarından balık sosu üretimi ve kalite özelliklerinin belirlenmesi. ÇOMÜ Ziraat Fak. Derg. 6:177-185
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Peralta EM, Endoma JrLF, Sefil AS, Palmos GN. 2021. Chemical and antioxidant activity changes in Philippine green mussel (Perna viridis) during fermentation at varying salt concentrations. Philipp J. Sci. 150(6B):1711-1722
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Pyo YH, Oh HJ. 2011. Ubiquinone contents in Korean fermented foods and average daily intakes. J. Food Compos. Anal. 24(8):1123-1129
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Ritthiruangdej P, Suwonsichon T. 2006. Sensory properties of Thai fish sauces and their categorization. Agric. Nat. Resour. 40(6):181-191
-
Russo GL, Langellotti AL, Genovese A, Martello A, Sacchi R. 2020. Volatile compounds, physicochemical and sensory characteristics of Colatura di Alici, a traditional Italian fish sauce. J. Sci. Food Agric. 100:3755-3764
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Sørensen R, Kildal E, Stepaniak L, Sørhaug T. 2004. Inhibition of angiotensin I converting enzyme (ACE) by peptides from fish muscle and cheese. Curr Top Pept Protein Res. 6:101-104
-
Sun J, Yu X, Fang B, Ma L, Xue C, Zhang Z, Mao X. 2016. Effect of fermentation by Aspergillus oryzae on the biochemical and sensory properties of anchovy (Engraulis japonicus) fish sauce. Int. J. Food Sci. Technol. 51(1):133-141
-
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. 2006. Comparison of ABTS, DPPH, FRAP and ORAC Assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal. 19:669-675
-
Wang Y, Li C, Li L, Yang X, Wu Y, Zhao Y, Wei Y. 2018. Effect of bacterial community and free amino acids on the content of biogenic amines during fermentation of Yu-lu, a Chinese fermented fish sauce. J. Aquat. Food Prod. 27(4):496-507
-
Zang J, Xu Y, Xia W, Yu D, Gao P, Jiang Q, Yang F. 2018. Dynamics and diversity of microbial community succession during fermentation of Suan yu, a Chinese traditional fermented fish, determined by high throughput sequencing. Food Res Int. 111:565-573
-
Zeng X, Xia W, Jiang Q, Yang F. 2013. Effect of autochthonous starter cultures on microbiological and physico-chemical characteristics of Suan yu, a traditional Chinese low salt fermented fish. Food Control. 33(2):344-351
The effect of Lactobacillus sakei and Staphylococcus carnosus bacteria on the antioxidant activity capacity in fish sauce production
Year 2025,
Volume: 8 Issue: 1, 1 - 7, 30.06.2025
Fatma Çolakoğlu
,
Selin Özge Dinç
,
Fatma Nur Ergün
Abstract
Fish sauce is a high-quality functional product produced through fermentation techniques. The structural properties of this amber-colored, flavorful liquid are shaped by the microorganisms and enzyme activities present in the environment. In this study, fish sauce was produced using anchovy (Engraulis encrasicolus) with traditional methods and starter cultures of Lactobacillus sakei and Staphylococcus carnosus (BFL-F06, Germany). The use of starters represents a production method that promises both a lower salt content and improved flavor development compared to traditional production. In the study, 20 % salt was used in traditional production and 3% in starter-added production, and the resulting product groups were also evaluated in terms of quality characteristics. In the sauce groups, the crude protein content was determined as 10.72-12.43 % and the crude fat content as 3.21-2.17 %, respectively. Sensory evaluation revealed that the starter culture group had a sour taste, while the traditional sauce group had a salty and fishy taste. In terms of DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, the traditional production method resulted in sauces with stronger antioxidant activity.
Supporting Institution
TÜBİTAK
Project Number
1919B012222851
References
-
Aluko R. 2012. Bioactive peptides. In: Functional foods and nutraceuticals, 1st edn. Springer, New York
-
Anonim 1998. Bacteriological analytical manual edition 8, revision A. In: Food and Drug Administration (FDA) Division of Microbiology and Association of Official Analytical Chemists. New Hampshire, USA
-
Anonim 2001. Bacteriological analytical manual. In: Food Drug Administration (FDA), Center for Food Safety & Applied Nutrition (CFSAN). New Hampshire, USA
-
AOAC 2000. Official Methods of Analysis. In: Association of Official Analytical Chemists, 18th edn. D.C., Washington
-
Bligh EG, Dyer WJ. 1959. A rapid method of lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917
-
Bu Y, Liu Y, Luan H, Zhu W, Li X, Li J. 2021. Changes in protease activity during fermentation of fish sauce and the correlation with antioxidant activity. J. Sci. Food Agric. 102(8):3150-3159
-
Duan S, Hu X, Li M, Miao J, Du J, Wu R. 2016. Composition and metabolic activities of the bacterial community in shrimp sauce at the flavor-forming stage of fermentation as revealed by metatranscriptome and 16S rRNA gene sequencings. J. Agric. Food Chem. 64(12):2591-2603
-
Elegado FB, Colegio SMT, Lim VMT, Gervasio ATR, Perez MTM, Balolong MP, Banaay CBG, Mendoza BC. 2016. Ethnic fermented foods of the Philippines with reference to lactic acid bacteria and yeasts. In: Tamang JP (ed) Ethnic fermented foods and alcoholic beverages of Asia. Springer, New York
-
Feng Y, Su G, Zhao H, Cai Y, Cui C, Sun-Waterhouse D, Zhao M. 2015. Characterisation of aroma profiles of commercial soy sauce by odour activity value and omission test. Food Chem. 167: 220-228
-
Hamzeh A, Noisa P, Yongsawatdigul J. 2020. Characterization of the antioxidant and ACE-inhibitory activities of Thai fish sauce at different stages of fermentation. J. Funct. Foods. 64:103699.
-
Harrigan WF, McCance ME. 1976. Laboratory methods, 2nd edn. Academic Press, London
-
Je JY, Park PJ, Byun HG, Jung WK, Kim SK. 2005. Angiotensin I converting enzyme (ACE) inhibitory peptide derived from the sauce of fermented blue mussel, Mytilus edulis. Bioresour. Technol. 96(14):1624-1629
-
Lee SH, Jung JY, Jeon CO. 2015. Bacterial community dynamics and metabolite changes in myeolchi-aekjeot, a Korean traditional fermented fish sauce, during fermentation. Int. J. Food Microbiol. 203:15-22
-
Lee YC, Kung HF, Huang CY, Huang TC, Tsai YH. 2016. Reduction of histamine and biogenic amines during salted fish fermentation by Bacillus polymyxa as a starter culture. J Food Drug Anal. Journal List. 24(1):157-163
-
Ludorf W, Meyer V. 1973. Fische und fisherzeugnisse. In: Auflage Z, Parey PY (eds). Berlin und Hamburg
-
Mueda RT. 2015. Physico-chemical and color characteristics of salt fermented fish sauce from anchovy stolephorus commersonii. AACL Bioflux. 8:565-572
-
O’Bryan CA, Crandall PG, Ricke SC, Ndahetuye JB. 2015. Lactic acid bacteria (LAB) as antimicrobials in food products: types and mechanisms of action. In: Taylor TM (ed) Handbook of natural antimicrobials for food safety and quality, Woodhead Publishing, USA
-
Ohata M, Uchida S, Zhou L, Arihara K. 2016. Antioxidant activity of fermented meat sauce and isolation of an associated antioxidant peptide. Food Chem. 194:1034-1039
-
Ormancı HB, Künili İE, Çolakoğlu FA. 2018. Alabalık (Oncorhynchus mykiss) ve atıklarından balık sosu üretimi ve kalite özelliklerinin belirlenmesi. ÇOMÜ Ziraat Fak. Derg. 6:177-185
-
Peralta EM, Endoma JrLF, Sefil AS, Palmos GN. 2021. Chemical and antioxidant activity changes in Philippine green mussel (Perna viridis) during fermentation at varying salt concentrations. Philipp J. Sci. 150(6B):1711-1722
-
Pyo YH, Oh HJ. 2011. Ubiquinone contents in Korean fermented foods and average daily intakes. J. Food Compos. Anal. 24(8):1123-1129
-
Ritthiruangdej P, Suwonsichon T. 2006. Sensory properties of Thai fish sauces and their categorization. Agric. Nat. Resour. 40(6):181-191
-
Russo GL, Langellotti AL, Genovese A, Martello A, Sacchi R. 2020. Volatile compounds, physicochemical and sensory characteristics of Colatura di Alici, a traditional Italian fish sauce. J. Sci. Food Agric. 100:3755-3764
-
Sørensen R, Kildal E, Stepaniak L, Sørhaug T. 2004. Inhibition of angiotensin I converting enzyme (ACE) by peptides from fish muscle and cheese. Curr Top Pept Protein Res. 6:101-104
-
Sun J, Yu X, Fang B, Ma L, Xue C, Zhang Z, Mao X. 2016. Effect of fermentation by Aspergillus oryzae on the biochemical and sensory properties of anchovy (Engraulis japonicus) fish sauce. Int. J. Food Sci. Technol. 51(1):133-141
-
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. 2006. Comparison of ABTS, DPPH, FRAP and ORAC Assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal. 19:669-675
-
Wang Y, Li C, Li L, Yang X, Wu Y, Zhao Y, Wei Y. 2018. Effect of bacterial community and free amino acids on the content of biogenic amines during fermentation of Yu-lu, a Chinese fermented fish sauce. J. Aquat. Food Prod. 27(4):496-507
-
Zang J, Xu Y, Xia W, Yu D, Gao P, Jiang Q, Yang F. 2018. Dynamics and diversity of microbial community succession during fermentation of Suan yu, a Chinese traditional fermented fish, determined by high throughput sequencing. Food Res Int. 111:565-573
-
Zeng X, Xia W, Jiang Q, Yang F. 2013. Effect of autochthonous starter cultures on microbiological and physico-chemical characteristics of Suan yu, a traditional Chinese low salt fermented fish. Food Control. 33(2):344-351