Assessment of Wild R. pimpinellifolia L. According to Mineral Content and Bioactive Compounds

Year 2021, Issue: 25, 644 - 649, 31.08.2021

Özlem Çakır , Berivan Ergen

https://doi.org/10.31590/ejosat.943477

Cited By: 2

Abstract

In this study the characteristics of the fruits of R. pimpinellifolia as a naturally grown and wild species in Bayburt was investigated. The antioxidant property of this fruit was determined by using four different methods; DPPH, ABTS, CUPRAC and β-carotene bleaching method in both water and methanolic extracts. Total phenolic content and phenolic profiles were determined by FCR (Folin-Ciocalteu reagent) method and HPLC, with respect. Additionally mineral contents and some physicochemical properties were analyzed. The antioxidant activities obtained from aqueous extract as 0.56 mmol TR/g-sample for CUPRAC, 97.02% for β-carotene bleaching method, 88.65% for ABTS and 86.69% for DPPH. For the methanol extraction antioxidant activities recorded as 0.99 mmol TR/g-sample for CUPRAC, 95.21% for β-carotene bleaching method, 93.43% for ABTS and 85.21% for DPPH. Total phenolic compound level was determined as 929.27 mg GAE/100 g in aqueous extract and that was found to be higher than methanolic extract. The most dominant phenolic compounds in the R. pimpinellifolia were detected as chlorogenic acid, vanillic acid and gallic acid. The dominant minerals found in this wild fruit were K (12.1688 mg/ml), P (1.3429 mg/ml) and Mg (1.1926 mg/ml) whereas Cr (0.00001 mg/ml) was minimum. Consequently, black rosehip (R. pimpinellifolia) fruit can be suggested to be very good natural nutrient source due to its high content of phenolic compounds and minerals and important antioxidant activity and phenolic.

Keywords

R. pimpinellifolia, Mineral content, Antioxidant activity, Phenolic profile, Total phenolic compound

Yabani Kara Kuşburnu (R. pimpinellifolia L.) Meyvesinin Mineral İçerik ve Biyoaktif Bileşenler Açısından Değerlendirilmesi

Abstract

Bu çalışmada Bayburt ilinde doğal olarak yetişen yabani R. pimpinellifolia L. meyvesinin genel özellikleri araştırılmıştır. Bu meyvenin antioksidan özellikleri dört farklı yöntem (DPPH, ABTS, CUPRAC ve β-karoten ağartma) kullanılarak hem su hem de metanol ekstrelerinde belirlenmiştir. Toplam fenolik madde içeriği ve fenolik profili sırası ile FCR (Folin-Ciocalteu ayıracı) metodu ve HPLC yardımıyla analiz edilmiştir. Bunlara ilaveten mineral madde içeriği ve bazı fizikokimyasal özellikleri ele alınmıştır. Su ekstresinde meyve antioksidan seviyeleri CUPRAC yönteminde 0.56 mmol TR/g-örnek, β-karoten ağartma yönteminde %97.02, ABTS metodunda %88.65 ve DPPH metodunda ise %86.69 olarak tespit edilmiştir. Metanol ekstresinde ise CUPRAC yönteminde 0.99 mmol TR/g-örnek, β-karoten yönteminde %95.21, ABTS metodunda %93.43 ve DPPH metodunda ise %85.21 olarak tespit edilmiştir. Toplam fenolik bileşen seviyesi ise sulu ekstresinde 929.27 mg GAE/100g olarak belirlenmiş olup bu değer metanol ekstresinde elde edilenden yüksektir. Meyvenin içerdiği fenolik bileşenler arasında en baskın olanların sırası ile klorojenik asit, vanilik asit ve gallik asit olduğu belirlenmiştir. Meyvede bulunan minerallerin arasında en yoğun olanlar sırası ile K (12.1688 mg/ml), P (1.3429 mg/ml) ve Mg (1.1926 mg/ml) olurken en az bulunan mineralin ise Cr (0.00001 mg/ml) olduğu açığa çıkartılmıştır. Sonuç olarak kara kuşburnu meyvesinin (R. pimpinellifolia) sahip olduğu bu özelliklerden dolayı çok iyi bir doğal besin olarak değerlendirilebileceği anlaşılmıştır.

Keywords

R. pimpinellifolia, Mineral madde, Antioksidan aktivite, Total fenolik madde içeriği, Fenolik profil

References

• Akkemik, Ü., 2018. Türkiye’nin Doğal-Egzotik Ağaç ve Çalıları. Orman Genel Müdürlüğü Yayınları, Ankara. 684.
• Alp, S., Ercisli, S., Jurikova, T., Cakir, O. and Gozlekci, S., 2016. Bioactive content of rose hips of different wildly grown Rosa dumalis genotypes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44(2):472-476.
• Apak, R., Güçlü, K., Demirata, B., Özyürek, M., Çelik, S. E., Bektaşoğlu, B., Berker, K.I. and Özyurt, D., 2007. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12:1496-1547.
• Barros, L., Carvalho, A.M. and Ferreira, C.F.R., 2011. Exotic fruits as a source of important phytochemicals: improving the traditional use of Rosa canina fruits in Portugal. Food Research International, 44:2233–2236.
• Boyd, P.D.A., 2012. Rosa spinosissima - aspects of its natural history and associations with people from prehistory to the present day. World Federation of Rose Societies 12th International Heritage Rose Conference, Sakura, Japan.
• Celik, F., Gundogdu, M., Alp, S., Muradoglu, F., Ercisli, S., Gecer, M. and Canan, I., 2017. Determination of phenolic compounds, antioxidant capacity and organic acids contents of Prunus domestica L., Prunus cerasifera Ehrh. and Prunus spinosa L. fruits by HPLC. Acta Chromatographica, 29(4):507-510.
• Cemeroglu, B. S. 2018. Meyve ve sebze işleme teknolojisi, 7th edn. Bizim Büro Basımevi, Ankara
• da Silva. A. P., Spricigo, P. C. & Purgatto, E., 2019. Volatile compounds determined by spme-gc, bioactive compounds, in vitro antioxidant capacity and physicochemical characteristics of four native fruits from South America. Plant Foods Human Nutrition, 74, 358-363.
• Dogan, A. and Kazankaya, A., 2006. Fruit properties of rose hip species Grown in Lake Van basin (Eastern Anatolia Region). Asian Journal of Plant Science, 5, 120-122.
• Ercisli, S., 2005. Rose (Rosa spp.) germplasm resources of Turkey. Genetic Resource Crop Evaluation, 52, 787–795.
• Fattahi, S. and Jamei Sarghein, S. H., 2012. Antioxidant and antiradical activities of Rosa canina and R. pimpinellifolia fruits from fest Azerbaijan Iran. Journal of Plant Physiology, 2(4): 523-529.
• Ghazghazi, M., Miguel, M.G., Hasnaoui, B., Sebei, H., Ksontini, M., Figueiredo, A.C., Pedro, L.G. and Barroso, J.G., 2010. Phenols, essential oils and carotenoids of Rosa canina from Tunisia and their antioxidant activities. African Journal of Biotechnology, 9(18):2709-2716.
• Guimaraes, R., Barros, L., Calhelha, R. C., Carvalho, A. M. and Queiroz, MJRP., 2014. Bioactivity of different enriched phenolic extracts of wild fruits from northeastern Portugal: a comparative study. Plant Foods Human Nutrition, 69, 37-42.
• Gulcin, I., 2005. The Antioxidant and radical scavenging activities of black pepper (Piper nigrum) seeds. International Journal of Food Science Nutrition, 56, 491-499.
• Gulcin, I., Mshvildadze, V. and Gepdiremen, Elias R., 2004. Antioxidant activity of Saponins isolated from ivy: a-hederin, hederasaponin-c, hederacolchiside e and hederacolchiside F. Planta Medica, 70:561–563.
• Gulcin, İ., Oktay, M., Kufrevioglu, I., and Aslan, A., 2002. Determination of antioxidant activity of lichen Cetraria islandica (L) Ach. Journal of Ethnopharmacology, 79(3): 325-329.
• Gundogdu, M., Ozrenk, K., Ercisli, S., Kan, T., Koda, O. and Hegedus, A., 2014. Organic acids, sugars, vitamin C content and some pomological characteristics of eleven hawthorn species (Crataegus spp.) from Turkey. Biological Research, 47, 1-5.
• Kasun, S., 2017. Determination of total phenolic content, phenolic composition, antioxidant capacities and some physicochemical features of rosehip (Rosa canina) and hawthorn (Crataegus orientalis) wild fruits growing in the region of Tunceli. Dissertation, Tunceli University, Tunceli, 115.
• Kaur, C. and Kapoor, H. C., 2002. Anti-oxidant activity and total phenolic content of some asian vegetables. International Journal of Food Scıence and Techology, 37(2):153-161.
• Kazankaya, A., Turkoglu, N., Yilmaz, M. & Balta, M. F., 2005. Pomological Description of Rosa canina Selections from Eastern Anatolia, Turkey. International Journal of Botany, 1, 100-102.
• Kazaz, S., Baydar, H., and Erbas S. 2009. Variations in chemical compositions of Rosa damascena Mill. and Rosa canina L. fruits. Czech Journal of Food Science, 27(3): 178-184.
• Kerasioti E, Apostolou A, Kafantaris I, Chronis K, Kokka E, Dimitriadou C, Tzanetou EN, Priftis A, Koulocheri SD, Haroutounian SA, Kouretas D. and Stagos D., 2019. Polyphenolic composition of Rosa canina, Rosa sempervivens and Pyrocantha coccinea extracts and assessment of their antioxidant activity in human endothelial cells. Antioxidants (Basel). 6:8(4):92.
• Keskin, T., 2000. İl Oluşunun 10. Yılında Bayburt Tarihi, Bayburt Valiliği Yayınları, Bayburt, s.1 Kovacs, S., Facsar, G., Laszlo, U. & Toth, M., 2004. Phenological, morphological characteristics of some rose species found in Hungary. Acta Horticulture, 690, 71–76.
• Macit, M., 2018. Determination of amounts and bioefficacy of phenolic compounds in roots of Rosa canina L. and Rosa pimpinelifolia Dissertation, İstanbul University, İstanbul, 92.
• Mavi, A., Terzi, Z., Ozgen, U., Yildirim, A. and Coskun, M., 2004. Antioxidant properties of some medicinal plants: Prangos Ferulacea (Apiaceae), Sedum Sempervivoides (Crassulaceae), Malva Neglecta (Malvaceae), Cruciata Taurica (Rubiaceae), R. pimpinellifolia (Rosaceae), Galium verum subsp. (Rubiaceae), Urtica dioica (urticaceae). Biological and Pharmaceutical Bulletin, 27(5):702-705.
• Meng, J., Fang, Y., Zhang, A., Chen, S., Xu, T., Ren, Z., Han, G., Liu, J., Li, H., Zhang, Z. and Wang, H., 2011. Phenolic content and antioxidant capacity of Chinese raisins produced in Xinjiang province. Food Research International, 44, 2830–2836.
• Murathan, Z. T., Zarifikhosroshahi, Kafkas, E. and Sevindik, E., 2016. Characterization of bioactive compounds in rosehip species from east Anatolia region of Turkey. Italian Journal of Food Science, 28, 314-325.
• Nadpal, J.D., Lesja, M., Sibul, F.S., Anacˇkov, G T., Cˇetojevic,´ -Simin D.D., Mimica-Dukic, N.M. and Beara, I.N., 2016. Comparative study of biological activities and phytochemical composition of two rose hips and their preserves: Rosa canina L. and Rosa arvensis Huds. Food Chemistry, 192, 907-914.
• Naveed, A., Farooq, A. and Anwar-ul-Hassan, G., 2015. Essential Oils in Food Preservation, Flavor and Safety. In: Victor R (ed) Rose Hip (Rosa canina L.) oils, 1st edn. Academic Press, Elsevier, England. 76, 667–675.
• Oz, M., 2016. The analysis of essential oil and biological activities from the flower, leaf, stem and fruit of rosehip species Rosa pimpinelifolia and Rosa canina L. Dissertation, Karadeniz Technical University, Trabzon, 192.
• Oz, M., Baltacı, C. and Deniz, I., 2018. The Analysis of Sugar and Vitamin C in Rosehip (Rosa canina L.) and Black rosehip (Rosa pimpinelifolia) Fruits of Gümüşhane Region. Gümüşhane University Journal of Science and Technology Institute, 8(2):284-292.
• Paunovic, D., Kalusevi, A., Petrovic, T., Urosevic, T., Djinovic, D., Nedovic, V. and Popovic-djordjevic, J., 2018. Assessment of chemical and antioxidant properties of fresh and dried rosehip (Rosa canina L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(1):108-113.
• Pizzale, L., Bortolomeazzi, R., Vichi, S., Uberegger, E. and Conte, L.S., 2002. Antioxidant activity of sage (Salvia officinalis and S fruticosa) and oregano (Origanum onites and O indercedens) extracts related to their phenolic compound content. Journal Science Food and Agriculture, 82, 1645-1651.
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice Evans, C., 1998. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231–1237.
• Rodriguez-Delgado, M. A., Malovana, S., Perez, J. P., Borges, T. and Garcia Montelongo, F. J., 2001. Separation of phenolic compounds by high-performance liquid chromatography with absorbance and fluorimetric detection. Journal of Chromatography A, 912(2):249-257.
• Savran, H. S., 1999. Organic acid profile of pomegranate juice. Dissertation, Ankara University, Ankara, 46.
• Su, L., Yin, J. J., Charles, D., Zhou, K., Moore, J. and Yu, L. L., 2007. Total phenolic contents, chelating capacities, and radical-scavenging properties of black peppercorn, nutmeg, rosehip cinnamon and oregano leaf. Food Chemistry, 100 (3): 990–997.
• Tarkan, M. T., 1974. Bayburt’un Ekonomik Olanakları ile Bu Olanakların Kalkınma Alanındaki Önem Dereceleri. Edebiyat Fakültesi Araştırma Dergisi, 5, 83-88.
• Yildiz, H., Cakır, O., Karatas, N., Oguz, H. I, Ciniviz, M. and Sasmaz, H. K., 2018. Polyphenol profile and antioxidant activity of some wild fruit in Turkey. The 4th Int. Symposium on Traditional Foods from Adriatic to Caucasus, Kyrenia / Northern Cyprus.
• Yolcu, H., 2010. Changes of antioxidant properties in rosehip pulp, Dissertation, Ondokuz Mayıs University, Samsun, 87.
• Yoo, K. M., Lee, C. H., Lee, H., Moon, B. and Lee, C. Y., 2008. Relative antioxidant and cytoprotective activities of common herbs. Food Chemistry, 106(3):929-936.