Chlorpyrifos and deltamethrin degradation potentials of two Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae) strains

Year 2020, , 165 - 176, 01.06.2020

Ayşegül Kumral , Nabi Alper Kumral , Ozan Gurbuz

https://doi.org/10.16970/entoted.625156

Cited By: 26

Abstract

Many soil bacteria can degrade the synthetic insecticides chlorpyrifos and deltamethrin by their esterase enzymes and/or by using them as carbon and energy sources. The hypothesis tested was that similar degradation potential could be found in Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae) which is used in food fermentations. This study was conducted in-vitro in Bursa Uludağ University laboratories during 2017-2018 to demonstrate the two degradation mechanisms of L. plantarum strains LB-1 and LB-2 4 d after inoculation. Significant growth in LB-1 found in mineral salt (MS) medium containing chlorpyrifos and deltamethrin compared with MS medium without insecticide and any carbon source. This strain also exhibited significantly enhanced hydrolysis activity. These capacities were found lower in LB-2 than LB-1. Based on periodically GC-MS analysis, degradation of chlorpyrifos and deltamethrin in MS medium proceeded by strains LB-1 and LB-2 reached the values of 96 and 90% and 24 and 53% after 3 d, respectively. Significant degradation of deltamethrin with both strains (86-82%) determined after 10 d. The study demonstrated that some L. plantarum strains could degrade chlorpyrifos and deltamethrin. Further studies should be conducted to show their effectiveness in the fermentation process of some fruits and vegetables and different bacteria inoculation rates.

Keywords

Degradation, esterase, insecticides, lactic acid bacteria, organophosphates, sythetic pyretroids

Supporting Institution

Bursa Uludag University, Scientific Research Unit, Bursa, Turkey

Project Number

OUB(Z)-2015/9.

Thanks

This project was supported by Bursa Uludağ University, Scientific Research Unit, Bursa, Turkey, Grant Project No: OUB(Z)-2015/9. A part of this research was orally presented in Eighth International Olive Symposium (Split, Croatia) with an abstract included in the proceedings.

İki Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae) suşunun chlorpyrifos ve deltamethrini parçalama potansiyelleri

Abstract

Birçok toprak bakterisi chlorpyrifos ve deltamethrin gibi sentetik insektisitleri esteraz enzimleriyle ve/veya bunları karbon ve enerji kaynağı olarak kullanarak parçalayabilmektedir. Bizim bu çalışmadaki hipotezimiz gıda fermantasyonu aşamalarında kullanılan Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae)’un benzer bir insektisit parçalama potansiyelinin gösterilmesidir. Bu çalışma,raroa ei Lactobacillus plantarum’un iki farklı suşunun (LB-1 ve LB-2) aşılamadan sonraki 4 gün içinde, iki farklı insektisit parçalama mekanizmasını göstermek amacıyla 2017-2018 yıllarında, Bursa Uludağ Üniversitesi laboratuvarlarında, in-vitro koşullarda gerçekleştirilmiştir. Herhangi bir karbon ve enerji kaynağı içermeyen MS ortamı ile karşılaştırıldığında, chlorpyrifos ve deltamethrin içeren MS ortamında önemli düzeyde LB-1 gelişimi saptanmıştır. Ayrıca, bu suş için önemli düzeyde artan hidroliz aktivitesi de gözlemlenmiştir. Bu özellikler LB-2’de bir miktar daha düşük bulunmuştur. GC-MS cihazı ile yapılan periyodik analizler sonucunda, LB-1 ve LB-2 inoküle edilmiş MS ortamı içinde chlorpyrifos ve deltamethrin’in parçalama oranları, 3 gün sonra chlorpyrifos için sırasıyla %96 ve 90, deltamethrin için %24 ve 53 olarak belirlenmiştir. Deltamethrin için önemli düzeyde bir parçalanma (%86-82) inkübasyondan 10 gün sonra gerçekleşmiştir. Bu çalışma, denemede kullanılan L. plantarum suşlarının chlorpyrifos ve deltamethrin parçalama potansiyellerinin olduğunu göstermiştir. İleride bazı meyve sebzelerin fermentasyon süreçlerinde kullanımı ve bu suşların farklı inokülasyon oranlarında etkinliğinin belirlenmesi amacıyla daha fazla çalışma yapılması gerekmektedir.

Keywords

Parçalanma, esteraz, insektisitler, laktik asit bakterileri, organik fosforlular, sentetik piretroitler

Project Number

OUB(Z)-2015/9.

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