İki Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae) suşunun chlorpyrifos ve deltamethrini parçalama potansiyelleri

Öz

Birçok toprak bakterisi chlorpyrifos ve deltamethrin gibi sentetik insektisitleri esteraz enzimleriyle ve/veya bunları karbon ve enerji kaynağı olarak kullanarak parçalayabilmektedir. Bizim bu çalışmadaki hipotezimiz gıda fermantasyonu aşamalarında kullanılan Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae)’un benzer bir insektisit parçalama potansiyelinin gösterilmesidir. Bu çalışma,raroa ei Lactobacillus plantarum’un iki farklı suşunun (LB-1 ve LB-2) aşılamadan sonraki 4 gün içinde, iki farklı insektisit parçalama mekanizmasını göstermek amacıyla 2017-2018 yıllarında, Bursa Uludağ Üniversitesi laboratuvarlarında, in-vitro koşullarda gerçekleştirilmiştir. Herhangi bir karbon ve enerji kaynağı içermeyen MS ortamı ile karşılaştırıldığında, chlorpyrifos ve deltamethrin içeren MS ortamında önemli düzeyde LB-1 gelişimi saptanmıştır. Ayrıca, bu suş için önemli düzeyde artan hidroliz aktivitesi de gözlemlenmiştir. Bu özellikler LB-2’de bir miktar daha düşük bulunmuştur. GC-MS cihazı ile yapılan periyodik analizler sonucunda, LB-1 ve LB-2 inoküle edilmiş MS ortamı içinde chlorpyrifos ve deltamethrin’in parçalama oranları, 3 gün sonra chlorpyrifos için sırasıyla %96 ve 90, deltamethrin için %24 ve 53 olarak belirlenmiştir. Deltamethrin için önemli düzeyde bir parçalanma (%86-82) inkübasyondan 10 gün sonra gerçekleşmiştir. Bu çalışma, denemede kullanılan L. plantarum suşlarının chlorpyrifos ve deltamethrin parçalama potansiyellerinin olduğunu göstermiştir. İleride bazı meyve sebzelerin fermentasyon süreçlerinde kullanımı ve bu suşların farklı inokülasyon oranlarında etkinliğinin belirlenmesi amacıyla daha fazla çalışma yapılması gerekmektedir.

Anahtar Kelimeler

• Parçalanma,esteraz,insektisitler,laktik asit bakterileri,organik fosforlular,sentetik piretroitler

Chlorpyrifos and deltamethrin degradation potentials of two Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae) strains

Abstract

Many soil bacteria can degrade the synthetic insecticides chlorpyrifos and deltamethrin by their esterase enzymes and/or by using them as carbon and energy sources. The hypothesis tested was that similar degradation potential could be found in Lactobacillus plantarum (Orla-Jensen, 1919) (Lactobacillales: Lactobacillaceae) which is used in food fermentations. This study was conducted in-vitro in Bursa Uludağ University laboratories during 2017-2018 to demonstrate the two degradation mechanisms of L. plantarum strains LB-1 and LB-2 4 d after inoculation. Significant growth in LB-1 found in mineral salt (MS) medium containing chlorpyrifos and deltamethrin compared with MS medium without insecticide and any carbon source. This strain also exhibited significantly enhanced hydrolysis activity. These capacities were found lower in LB-2 than LB-1. Based on periodically GC-MS analysis, degradation of chlorpyrifos and deltamethrin in MS medium proceeded by strains LB-1 and LB-2 reached the values of 96 and 90% and 24 and 53% after 3 d, respectively. Significant degradation of deltamethrin with both strains (86-82%) determined after 10 d. The study demonstrated that some L. plantarum strains could degrade chlorpyrifos and deltamethrin. Further studies should be conducted to show their effectiveness in the fermentation process of some fruits and vegetables and different bacteria inoculation rates.

Keywords

• Degradation,esterase,insecticides,lactic acid bacteria,organophosphates,sythetic pyretroids

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