Çanakkale açık pazarlarından örneklenen hıyarlarda insektisit kalıntı analizleri

Öz

Bu çalışma hıyarlarda dört insektisit kalıntısını QuEChERS 2007.1 yöntemi ile belirlemek amacıyla yapılmıştır. Metot doğrulama değerlendirmesi için pestisit içermeyen hıyar örneği MRL değerlerinin 0.1, 1 ve 10 katı seviyelerinde her pestisit ile zenginleştirilmiştir (fortifikasyon). QuEChERS-LC-MS/MS analiz yöntemi ile insektisitlerin dedeksiyon limitleri MRL’lerin altında ve tüm metodun geri alımı %97.7 olarak bulunmuştur. Bu değerler SANTE geri alım limiti (%60-140) ve belirlenen tekrarlanabilirlik değerleri (≤20%) arasındadır. Hıyarlar 6 hafta boyunca Çanakkale açık pazarlarından altı farklı tezgâhtan (A-F) 23 Kasım-28 Aralık 2018 tarihleri arasında toplanmıştır. Her bir örnekleme zamanı ve her bir tezgâha ait örneklerde kalıntılar araştırılmıştır. Acetamiprid kalıntısı, 5. hafta B tezgahında ve 2. hafta E tezgahında sırasıyla 257 µg/kg ve 236 µg/kg olarak tespit edilmiştir. Bu değerler MRL‘ne (300 µg/kg) yakındır. Üçüncü hafta F tezgahında formetanate hidroklorür kalıntısı (36.3 µg/kg),10 µg/kg MRL değerinden daha fazla bulunmuştur. Hıyarlarda pirimiphos methyl ve chlorpyrifos kalıntısı bulunmamıştır. Teorik maksimum günlük alım değerlendirmesi hıyar tüketiminde bu 4 pestisitin kronik maruziyet riski oluşturmadığını göstermiştir.

Anahtar Kelimeler

• Hıyar
• insektisit kalıntıları
• QuEChERS
• risk değerlendirmesi
• toksikoloji

Insecticide residue analyses in cucumbers sampled from Çanakkale open markets

Abstract

The aim of this study was to investigate four insecticide residues in cucumbers with the aid of QuEChERS 2007.1 method. For method verification assessment, pesticide-free cucumber matrix was spiked with 0.1, 1 and 10 times of MRL for each pesticide. The QuEChERS-LC-MS/MS analytical method revealed that the detection limits of the insecticides were below the MRLs and the overall recovery of method was 97.7%. These figures were within the SANTE recovery limits (60-140%) and the values specified for the repeatability (≤20%). Cucumbers were collected from six different stands (A-F) at Çanakkale open markets for 6 weeks between 23 November and 28 December 2018. Residues of each sampling time and each stand were assessed. Acetamiprid residue of 257g and 236 µg/kg were detected in week 5 from stand B and in week 2 from stand E, respectively. These values are close to MRL (300 µg/kg). Formetanate hydrochloride residue of the week 3 from stand F (36.3 µg/kg) was more than MRL of 10 µg/kg. Pirimiphos methyl and chlorpyrifos residues were not detected in cucumbers. Theoretical maximum daily intake assessment showed that there was no chronic exposure risk for these four pesticides through cucumber consumption.

Keywords

• Cucumber
• insecticide residues
• QuEChERS
• risk assessment
• toxicology

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