Combine Effect of Vitamin C and Cadmium on the Aminoacid Content of Citrobacter Freundii

Year 2021, Volume: 14 Issue: 3, 1023 - 1038, 18.12.2021

Muhammad Salihu Ibrahim , Meltem Çakmak , Dursun Özer , Fikret Karataş , Sinan Saydam

https://doi.org/10.18185/erzifbed.1004425

Abstract

Bu çalışmada, Citrobacter freundii farklı konsantrasyonlarda kadmiyum içeren LB besi yerinde üretildi. Daha sonra kadmiyum içeren besi yerine değişik konsantrasyonlarda C vitamini katılarak, her iki durumda da üretilen bakterilerdeki aminoasit miktarları HPLC ile belirlendi. Kontrol ile 75, 100 ve 125 ppm Cd içeren gruplar karşılaştırıldığında alanin, arginin, asparajin, aspartik asit, sistein, glisin, histidin, isoleusin, leusin, lisin, fenilalanin, prolin, treonin, tirozin ve valin aminoasitlerinin miktarlarında artış (p<0.05), glutamik asit, glutamin, metiyonin, serin ve triptofan miktarlarında ise azalma p<0.05) gözlendi.
Besi ortamına ilave edilen C vitamini sonucunda, kadmiyumun etkisine zıt etki göstererek miktarları artan aminoasitlerde azalma, miktarları azalan aminoasitlerde ise artış gözlenmiştir. Bu bulgulardan, kadmiyumun C. freundii üzerindeki olumsuz etkisini, besi yerine katılan C vitaminin azalttığı söylenebilir.

Keywords

C. Freundii, Cadmium, Vitamin C, Aminoacid

Project Number

Yok

References

• Al-Ghafari, A., Elmorsy, E., Fikry, E., Alrowaili, M., Carter, W.G., & Mukhopadhyay, P. 2019. "The heavy metals lead and cadmium are cytotoxic to human bone osteoblasts via induction of redox stressʺ. Plos One, 14(11), e0225341
• Alreshidi, M.M., Dunstan, R.H., Macdonald, M.M., Smith, N.D., Gottfries, J., & Roberts, T.K. 2019. "Amino acids and proteomic acclimation of Staphylococcus aureus when incubated in a defined minimal medium supplemented with 5% sodium chlorideʺ. Microbiologyopen, C 8, e00772.
• Azevedo, R.A., & Lea, P.J. 2001. "Lysine metabolism in higher plantsʺ. Amino Acids, 20(3), 261-279.
• Banfalvi, G. 2011. "Cellular Effects of Heavy Metalsʺ. Netherlands, London, New York: Springer. ISBN 978-94-007-0428-2
• Beyersmann, D., & Hartwig, A. 2008. "Carcinogenic metal compounds: recent insight into molecular and cellular mechanismsʺ. Archives of toxicology, 82, 493-51
• Chen, T.H., & Murata, N. 2008. "Glycinebetaine: an effective protectant against abiotic stress in plantsʺ. Trends of Plant Science, 13(9), 499-505.
• Davidson, J.A. 2019. "Amino Acids in Life: A Prebiotic Division of Laborʺ. J Mol Evol. https://doi.org/10.1007/s00239-018-9879-z.
• Demain, A.L., & Vaishnav, P. 2009. "Production of recombinant proteins by microbes and higher organismsʺ. Biotechnol Adv, 27: 297-306.
• Devaki, S.J., & Raveendran, R.L. 2017. "Vitamin C: Sources, Functions, Sensing and Analysisʺ. INTECH http://dx.doi.org/10.5772/intechopen.70162
• Elkin, R.G., & Wasynczuk, A.M. 1987. "Amino Acid Analysis of Feedstuff Hydrolysates by Precolumn Derivatization with Phenylisothiocyanate and Reversed-Phase High-Performance Liquid Chromatographyʺ. Cereal Chemistry 64(4), 226-229.
• Fersht, A. 1999. "Structure and Mechanism in Protein Scienceʺ. W.H. Freeman and Company; New York.
• Forde, B.G., & Lea, J.F. 2007. "Glutamate in plants: metabolism, regulation, and signallingʺ. Journal of Experimental Botany. 58(9), 2339-2358
• Ghelis, T. 2011. "Signal processing by protein tyrosine phosphorylation in plantsʺ. Plant Signaling & Behavior, 6(7), 942-951.
• Hamza, Amal H. 2017. "Vitamin C || Vitamin C: Sources, Functions, Sensing and Analysisʺ. 10.5772/66058(Chapter 1), DOI:10.5772/intechopen.7016
• Haroun, S.A., Shukry, W.M., & El-Sawy, O. 2010. "Effect of asparagine or glutamine on growth and metabolic changes in phaseolus vulgaris under in vitro conditionsʺ. Bioscience Research, 7(1), 1-21.
• Husein, A.S. 2012. "Genetic Relationship Between Heavy metals Resistance and B. Lactamase production in E.coli and Staphylococcus aureusʺ. Ibn ALHaitham Journal for pure and Applied Science. 25(1).
• Joshi, V., Joung, J.G., Fei, Z., & Jander, G. 2010. "Interdependence of threonine, methionine and isoleucine metabolism in plants: accumulation and transcriptional regulation under abiotic stressʺ. Amino Acids. 39(4), 933-947.
• Kalefetoğlu, T., & Ekmekçi, Y. 2005. "Bitkilerde kuraklık stresinin etkileri ve dayanıklılıkʺ. G.Ü. Fen Bilimleri Dergisi, 18(4), 723-740.
• Kwanyuen, P., & Burton, J.W. 2010. "A Modified Amino Acid Analysis Using PITC Derivatization for Soybeans with Accurate Determination of Cysteine and Half-Cystineʺ. Journal of the American Oil Chemists' Society 87(2), 127–132.
• Miflin, B.J., Habash, D.Z. 2002. "The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of cropsʺ. Journal of Experimental Botany., 53(370), 979- 987.
• Neuberg, M., Pavlikova, D., Pavlik, M., & Balik, J. 2010. "The effect of different nitrogen nutrition on praline and asparagine content in plantʺ. Plant Soil Environ, 56, 305–311
• O'Hara, C.M., Westbrook, G.L., & Miller, J.M. 1997. "Evaluation of Vitek GNI+ and Becton Dickinson Microbiology Systems Crystal E/NF identification systems for identification of members of the family Enterobacteriaceae and other gram-negative, glucose-fermenting and non-glucose-fermenting bacilliʺ. Journal of Clinical Microbiology, 35, 3269-3273.
• Olgun, M., Budak Başçiftçi, Z., Ayter, N.G., Turan, M., Aydın, D., Şaban, Z., Ali Cevat Sönmez, A.C., Koyuncu. O. 2016. "Potasyum Iyodür Uygulamasının Ekmeklik Buğday Çeşitlerinin Biyokimyasal Özellikleri Üzerine Etkisiʺ. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi 11 (2), 46-60,
• Paschal, D., Burt, V., Caudill, S., Gunter, E. W., Pirkle, J.L., Sampson, E. J., Miller, D.T. & Jackson, R.J. 2000. "Exposure of the US population aged 6 years and older to cadmium: 1988–1994ʺ. Archives of environmental contamination and toxicology, 38, 377-383.
• Paul, M.J., & Driscoll, S.P. 1997. "Sugar repression of photosynthesis: the role of carbohydrates in signalling nitrogen deficiency through source: sink imbalanceʺ. Plant Cell Environx 20, 110–116.
• Pavlíková, D., Pavlík, M., Staszková, L., Motyka, V., Száková, J., Tlustoš, P., & Balík, J. 2008. "Glutamate kinase as a potential biomarker of heavy metal stress in plantsʺ. Ecotoxicology and Environmental Safety. 70(2), 223-230.
• Puchenkova, S., 1996. "Enterobacteria in areas of water along the Crimean Coastʺ. Mikrobiolohichnyi Zhurnal (Kiev, Ukraine: 1993), 58, 3-7.
• Ros, R., Bertomeu, J.M., & Krueger, S. 2014. "Serine in plants: biosynthesis, metabolism, and functionsʺ. Trends in Plant Science, 19(9), 564-569.
• Sahiti, H., Bislimi, K., Rexhepi, A., & Dalo, E. 2020. "Metal Accumulation and Effect of Vitamin C and E in Accumulated Heavy Metals in Different Tissues in Common Carp (Cyprinus carpio) Treated with Heavy Metalsʺ. Polish Journal of Environmental Studies. 29(1), 799-805.
• Sanchez, F.J., Manzanares, M., Andres, E.F., Tenorio, J.L., & Ayerbe, L. 1998. "Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stressʺ. Field Crops Res., 59, 225-235.
• Singhl, B.K., & Shaner, D.L. 1995. "Biosynthesis of branched chain amino acids: From test tube to fieldʺ, The Plant Cell, 7, 935-944.
• Tohge, T., Watanabe, M., Hoefgen, R., & Fernie, A.R. 2013. "Shikimate and phenylalanine biosynthesis in the green lineageʺ. Front Plant Sci., 4, 62.
• Xu, J., Sun, J., Du, L., & Liu, X. 2012a. "Comparative transcriptome analysis of cadmium responses in Solanum nigrum and Solanum torvumʺ. New Phytol. 196(1), 110–124.
• Yang, H.Q., & Gao, H.J. 2007. "Physiological function of arginine and its metabolites in plantsʺ. Journal of Plant Physiology and Molecular Biology. 33(1), 1-8.
• Wang, J., Chang, S., Chen, Y., & Luh, K., 2000. "Comparison of antimicrobial susceptibility of Citrobacter freundii isolates in two different time periodsʺ. Journal of Microbiology, Immunology and Infection. 33, 258-262.
• Wu, G. 2010. "Functional amino acids in growth, reproduction, and healthʺ. Advances in Nutrition, C 1, 31-37
• Wu, G. 2013. "Functional amino acids in nutrition and healthʺ, Amino Acids 45(3), 407–411.
• Zemanova´ V, Pavlı´k M, Pavlı´kova´ D. 2017. "Cadmium toxicity induced contrasting patterns of concentrations of free sarcosine, specific amino acids and selected microelements in two Noccaea speciesʺ. Plos One 12(5), e0177963.