Biyolojik Meteryalde Değişik Metotlarla Striknin Aranması

Year 1994, Volume: 7 Issue: 5, 79 - 98, 01.12.1994

Niyazi Savaş

Abstract

Bu çalışmada ülkemizdeki toksikoloji laboratuvarlarında striknin analizi
için kullanılabilecek en çabuk, kolay, güvenilir ve ucuz yöntemin seçilmesi
amacıyla ince tabaka kromotografi, kağıt kromotografi, renk testi ve
GC/MS yöntemleri karşılaştırmalı olarak çalışıldı.Ince tabaka kromotografi Rf değeri, 0.22, yarı-nieel olarak gerçekleştirilen
rekoveri oranı % 78 ve hassasiyet sınırı 0.1 ppm olarak, kağıt kromotografi
yöntemi Rf değeri 0.30 ve hassasiyet sınırı 1 ppm olarak, renk
testi hassasiyet sınırı 0.5 ı-ıg olarak ve GC/MS sisteminde nice! rekoveri
oranı % 88, hassasiyet sınırı ise 1 O ppb olarak belirlendi.
Ülkemizdeki toksikoloji laboratuvarlarında bulunabilecek alet ve malzeme
ile yapılabilmesi, çabuk, ucuz, kolay ve güvenilir olması nedeniyle ince
tabaka kromotografi yönteminin rutin olarak kullanılabileceği kanaatine
varılmıştır. Ayrıca GC/MS sistemi de aynı anda birçok maddenin analizinin
gerçekleştirilebilmesine olanak sağladığından rutin doping analizlerinde tercih edilebilecek bir metot olarak görünmektedir.

Keywords

Striknin, Biyolojik Maddelerde Striknin Aranması, Niyazi SAVAŞ, 1994, Etlik Veteriner Mikrobiyoloji Dergisi, Zehir, Toksikoloji, Doping

«Detection of strychnine in biological materials by using various techniques»

Abstract

In this study, the analysis of strychnine was carried out by using thnilayer
chromotography, paper chromotography, colour test and GC/MS system
to determine a rapid, easy, reliable and cheap method applicable to to-xicology laboratories.
lt was found that the Rf, semi-quantitative recovery and detection limit
for thi-lyaer chromotography were 0.22, 78 % and 0.1 ppm, and for paper
chromotography Rf and detection limit were 0.30 and 1 ppm respectively.
Colour test's detetion limit was 0.5 μg . Ouantitative recovery and
detection limit for GC/MS were 78% and 10 ppb respectively.
lt is concluded that thin-layer chromotography is applicable to toxicology
laboratories, because it is rapid, easy to perform, reliable and applicable
with the equipment and glassware present in all toxicology laboratories.
In addition, GC/MS system which enables the analysis of many
substances at the same time can be preferrev for routine doping analysis.

References

• ALEXANDER, F. : Veteriner Farmakoloji. Ankara Üniv. Vet. Fak. Yay. No. 308. Yardımcı Ders Kitabı No. 209. s. 136-139. "Çeviri" Özkazanç, A.N. ve Ceylan, S. 1969.
• AKMAN, M.S., GÜRTUNCA, Ş. , OZAN, K. ve CEYLAN, S. : Letal doz tayini yoluyla eski bir striknin sülfat numunesinde aktivitenin incelenmesi. Ankara Üniv. Vet. Fak. Derg., 19 (3): 413-418. 1972.
• BOOTH, N.H. and McDONALD, l.E. : Veterinary Pharmacology and Therapeutics. Sixth Edition. Oowa State University Press. pp. 1073-1075. 1988.
• CLARKE, E.G.C. : Isolation and ıdentification of Drugs in Pharmaceuticals Body Fluids and Post-mortam Material. the Pharmaceutical Press. pp. 33-37
• CZARNY. R.J. and HORNBECK, C.l. : Quantitation of methamphetamine and amphetamine in urine by capillary GS/MS Part ll: Derivatization with 4-carbethoxyhexafluorobutyrly chlorid. J. Anal. Tox. 13: 257-262. 1989.
• DONIKE, M. : The detection of doping agents in blood. Br. J. Sports Med., 10 (3): 147- 154. 1976.
• EHRESMAN, J.D., PRICE, M.S. and LAKATUS, J.D. : Screning biological samples for underivatized drugs using a splitless injection technique on fused slica capillary column gas chromatography. J. Anal. Tox., 9: 55-62. 1985.
• ELSOHL Y, M.A., ELSOHLY, H.N. JONES, A.B. , DIMSON, P.A. and WELLS, K.A. : GC/MS canfirmation of stimulats. J. Anal. tox. 1983.
• FOLTZ, R.L., FENTIMAN, A.F. and FOLTZ, R.B. : GC/MS assays for abused drugs in body fluid. Nationalinstitute on Drug abuseResearch Monograph 32. pp. 175. 1980.
• FOX, R.H. : Paper Chromatography. In: Clarke, E.G.C.: Isolation and ldentification of Drugs in Pharmaceuticals Body Fluids and Post-mortam Material. The Pharmceutical Press. pp. 31-42. 1969.
• GRADWOHL, R.B.H. : Clinical Laboratory Methods and Diagnosis. Fifth edition. the C. V. Mosby Com. s. 2320-2323. 1956.
• GILMAN, A.G., GOODMAN, L.S. and GILMAN, A. : Goodman and Gilman's The Pharmacological Basis of Therapeutics. Sixth edition. MacMillan Publishing Co., pp. 1072-1075.1980.
• KANDEMIR, 1. : Tıbbi toksikoloji. Ankara Üniv. Tıp Fak. Yay. s. 158-164. 1960.
• KENYON, C.N., MELERA, A. and ERNI , F. : Use of the direct liquid inlet LC/MS system for the analysis of complex mixtures of biologica origin. J. chromatogr. Sci., 18: 103-104. 1980.
• MAYLIN, A.G., DEWEY, A.E. and HANION, D.J. : Determination of drugs in equine urine by gas chromatography-fourier transform infrarred spectroscopy. Equine Drug Teslig and Toxicology, New York State College of Veterinary MediCine, Comeli Universty. 1986.
• MOFFAT, A.C., JACSON, J.V. MOSS, M.S. and WIDDOP, B.: Clarke's Isolation and ldetification of Drugs in Pharmaceuticals Body Fluids ad Post-mortam Material. Second Edition. London Pharmaceutical Press. pp. 976,977. 1986.
• NıCHOLSON, J.A. : Lander's Veterinary Toxicology. Third Edition. Bailliere, Tındali andCox. pp. 123-124. 1947.
• OGURI, K., TANIMOTO, V., MISHIMA, M. and VOSHIMURA, H. : Matabolic fate of strychnine in ra ts, Fa c. Pharma. Sci., Kyushu Univ. 19 (2): 171-178. 1989.
• STHAL, E. : Thin-Layer Chromotography. A Laboratory. Handbook. Second edition. Springer-Verlag Berlin. pp. 446-449. 1969.
• SUNSHINE, 1. : CRC Handbook Series in Analytical Toxicology. CRC press. pp. 771. 1979.
• ŞANLI, Y. ve KAYA, S. : Veteriner Farmokoloji ve Ilaçla Sağıtım Seçenekleri. Medisan Yay. No. 4 s. 247-249. 1991.
• ŞANLI, Y. ve KAYA, S.: Veteriner Klinik Toksikoloji. Medisan Yay. No. 5. s. 175-178.
• WATTS, V. and SIMONICK, F.T. : Screning of basic durgs in biological samples using dual column capillary chromatography and nitrogen-phoshorus detectors. J. Anal. Tox. 10: 198-204. 1986.
• WIDDOP, B. : Hospital toksikology and drug abuse screning. In: Moffat, A.C., Jacson, J.V., Mess, M.S. and Widdop, 8.: Clarke's Isolatic and ldentification of Drugs in Pharmaceuticals Body Fluids and Post-mortam Matarial Second Edition. London Pharmaceutical Press. pp. 3-20.