Biyolojik Meteryalde Değişik Metotlarla Striknin Aranması

Yıl 1994, Cilt: 7 Sayı: 5, 79 - 98, 01.12.1994

Niyazi Savaş

Öz

Bu çalışmada ülkemizdeki toksikoloji laboratuvarlarında striknin analizi
için kullanılabilecek en çabuk, kolay, güvenilir ve ucuz yöntemin seçilmesi
amacıyla ince tabaka kromotografi, kağıt kromotografi, renk testi ve
GC/MS yöntemleri karşılaştırmalı olarak çalışıldı.Ince tabaka kromotografi Rf değeri, 0.22, yarı-nieel olarak gerçekleştirilen
rekoveri oranı % 78 ve hassasiyet sınırı 0.1 ppm olarak, kağıt kromotografi
yöntemi Rf değeri 0.30 ve hassasiyet sınırı 1 ppm olarak, renk
testi hassasiyet sınırı 0.5 ı-ıg olarak ve GC/MS sisteminde nice! rekoveri
oranı % 88, hassasiyet sınırı ise 1 O ppb olarak belirlendi.
Ülkemizdeki toksikoloji laboratuvarlarında bulunabilecek alet ve malzeme
ile yapılabilmesi, çabuk, ucuz, kolay ve güvenilir olması nedeniyle ince
tabaka kromotografi yönteminin rutin olarak kullanılabileceği kanaatine
varılmıştır. Ayrıca GC/MS sistemi de aynı anda birçok maddenin analizinin
gerçekleştirilebilmesine olanak sağladığından rutin doping analizlerinde tercih edilebilecek bir metot olarak görünmektedir.

Anahtar Kelimeler

Striknin, Biyolojik Maddelerde Striknin Aranması, Niyazi SAVAŞ, 1994, Etlik Veteriner Mikrobiyoloji Dergisi, Zehir, Toksikoloji, Doping

«Detection of strychnine in biological materials by using various techniques»

Öz

In this study, the analysis of strychnine was carried out by using thnilayer
chromotography, paper chromotography, colour test and GC/MS system
to determine a rapid, easy, reliable and cheap method applicable to to-xicology laboratories.
lt was found that the Rf, semi-quantitative recovery and detection limit
for thi-lyaer chromotography were 0.22, 78 % and 0.1 ppm, and for paper
chromotography Rf and detection limit were 0.30 and 1 ppm respectively.
Colour test's detetion limit was 0.5 μg . Ouantitative recovery and
detection limit for GC/MS were 78% and 10 ppb respectively.
lt is concluded that thin-layer chromotography is applicable to toxicology
laboratories, because it is rapid, easy to perform, reliable and applicable
with the equipment and glassware present in all toxicology laboratories.
In addition, GC/MS system which enables the analysis of many
substances at the same time can be preferrev for routine doping analysis.

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