Kanatlıların IBV-Enfeksiyöz Bronşitis Hastalığına Karşı H-120 ve H-52 Suşları ile Canlı, Liyofilize, Pilot Aşı Hazırlanması

Year 1995, Volume: 8 Issue: 1, 218 - 229, 01.12.1995

Ragıp Bayraktar Canan Tayhanı M. Zafer Zoğ Salih Çiçek

Abstract

Bu araştırmada embriyolu SPF yumurtada liyofilize Mass. tipi H-120 ve H-
52 Enfeksiyöz Bronşitis virüs suşları ile aşı üretilip, kanatlıların bu hastalığa
karşı kullanılması amaçlanmıştır.
Canlı, liyofilize, Mass. tipi H-120 ve H-521BV suşları 9-11 günlük embriyolu
SPF yumurtaya corio :ıllantoik yolla in kule edilmiş, 36-48 saatler arası amnioallantoik
sıvı toplanmış , sterilite ve titre kontrolleri yapıldıktan sonra %2-3
oranında tindalize edilmiş süt tozu ilave edilmiş ve 1000 doz olacak şekilde
ampullere taksim edilerek liyofilize edilen ve -20 °C'Iik deep-freeze odalarda
saklanmaktadır. (20,23)
Aşılar şu anda istekler doğrultusunda +4 °C'de özel ambalajlarında tüm
yurda sevk edilmekte olup üretimimiz tüm ülkenin ihtiyacına cevap verecek
durumdadır.

Keywords

Kanatlı, IBV-Enfeksiyöz Bronşitis Hastalığı, H-120, H-52, Ragıp BAYRAKTAR, Canan TAYHANI, M.Zafer ZOĞ, Salih ÇİÇEK, 1995, Etlik Veteriner Mikrobiyoloji Dergisi

Preparation of live Lyophilized, pilot vaccine with H-52 strain against IBV Infections Bronchitis Disease of poultry

Abstract

The Study on live, Lyophilized IBV vaccines production by using IBV H-
120 and H-52 strains.
In this research; Vaccine production against to lnfections Bronchitis disease
had been aimed by the live, freeze-dried , Mass. type H-120 and H-52
IBV strains had been inoculated to 9-11 days old embriyoted SPF eggs by the
corio-allontoic route .After 36-48 hours, the virus content. 2-3 % tyndalizated
ski m med m ilk powder is ad d ed to vaccine harvest and lyophilized . Lyophilized
vaccines are stored in the -20 oc deep-freeze room . (20 ,23)
According to demands, the vaccines have been dispatching to all over the
country, in the special boxes at +4°C.

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