Kanatlıların IBV-Enfeksiyöz Bronşitis Hastalığına Karşı H-120 ve H-52 Suşları ile Canlı, Liyofilize, Pilot Aşı Hazırlanması

Yıl 1995, Cilt: 8 Sayı: 1, 218 - 229, 01.12.1995

Ragıp Bayraktar Canan Tayhanı M. Zafer Zoğ Salih Çiçek

Öz

Bu araştırmada embriyolu SPF yumurtada liyofilize Mass. tipi H-120 ve H-
52 Enfeksiyöz Bronşitis virüs suşları ile aşı üretilip, kanatlıların bu hastalığa
karşı kullanılması amaçlanmıştır.
Canlı, liyofilize, Mass. tipi H-120 ve H-521BV suşları 9-11 günlük embriyolu
SPF yumurtaya corio :ıllantoik yolla in kule edilmiş, 36-48 saatler arası amnioallantoik
sıvı toplanmış , sterilite ve titre kontrolleri yapıldıktan sonra %2-3
oranında tindalize edilmiş süt tozu ilave edilmiş ve 1000 doz olacak şekilde
ampullere taksim edilerek liyofilize edilen ve -20 °C'Iik deep-freeze odalarda
saklanmaktadır. (20,23)
Aşılar şu anda istekler doğrultusunda +4 °C'de özel ambalajlarında tüm
yurda sevk edilmekte olup üretimimiz tüm ülkenin ihtiyacına cevap verecek
durumdadır.

Anahtar Kelimeler

Kanatlı, IBV-Enfeksiyöz Bronşitis Hastalığı, H-120, H-52, Ragıp BAYRAKTAR, Canan TAYHANI, M.Zafer ZOĞ, Salih ÇİÇEK, 1995, Etlik Veteriner Mikrobiyoloji Dergisi

Preparation of live Lyophilized, pilot vaccine with H-52 strain against IBV Infections Bronchitis Disease of poultry

Öz

The Study on live, Lyophilized IBV vaccines production by using IBV H-
120 and H-52 strains.
In this research; Vaccine production against to lnfections Bronchitis disease
had been aimed by the live, freeze-dried , Mass. type H-120 and H-52
IBV strains had been inoculated to 9-11 days old embriyoted SPF eggs by the
corio-allontoic route .After 36-48 hours, the virus content. 2-3 % tyndalizated
ski m med m ilk powder is ad d ed to vaccine harvest and lyophilized . Lyophilized
vaccines are stored in the -20 oc deep-freeze room . (20 ,23)
According to demands, the vaccines have been dispatching to all over the
country, in the special boxes at +4°C.

Kaynakça

• A.J . BROWN. C. D. BRACEWEL, 1985: Application of the haemagglutination inhibition test to typhing of infectious brochitis virus. Veterinary Record, 166: 47-48.
• A.P.A. MOCKETT, JANE K.A. COOK and M.B. HUGGINS, 1987: Maternally- derivied antibody to lnfectious Bronchitis virus: lt's detection in chick trachea and serum and its role in protection. Avian Pathology, 16: 407-416.
• A .. J.BROWN, C.D.BRACEWELL, 1988: Effect of repeated infections of chickens with lnfectious bronchitis viruses on the specifity of their antibody responses. Veterinary record . 122: 707-708.
• C. PEJKOVSKI, F.G.DAVELAAR and B.KOUWENHOVEN, 1979: lmmunnosuppresive effect of lnfectious Bursa! disease virus on vaccination against infectious Bronchtis. Avian Pathology. 8:95-106.
• D.J.ALEXANDER and N.J .CHETTLE, 1977: Procedures for the hacmagglutinasyon and the haemagglutination in h ibition testsfor avi an lnfections bronchitis virus. Avian Pathology, 6:9-17.
• F.G. DAVELAAR, A.NOORDZIJ and J.A.VAN DER DONK, 1982: A study on the synthesis and seeretian of immunoglibulins by the harderian gland of the fowl after drop vaccination against lnfect ious Bronchitis at 1 dayold. Avian Pathology. 11 : 63,79.
• GORDON and JORDON, 1984: lnfectious Bronchitis diaease. Poultry Disease, 2. nd. Edition, 164-172.
• H. WILLIAMS SMITH, JANE K.A. COOK and ZÖE E.PARSELL, 1985: The experimantal infcction of chickens with mixtures of infectious Bronchitis virus and E.coli. J.Gen.Virol. 66: 777-786
• HOUGHTON POULTRY RESEARCH STATION-HPRS, UK, 1987: lnfectious Bronchitis laboratuvarı standart ve metotları .
• International Association of Biological Standardization, 1973: Report of the Avi an product standardization committe. 1 :12-15.
• JANE K.A. COOK, J.H. DARBYSHIRE and R.W. PETIERS, 1976: The use of chicken tracheal organ cultures for the isolation and assay of Avi an lnfectious Bronchitis virus. Archives of Virology. 50: 109-118.
• JANE K.A. COOK, 1984: The classification of new serotypes on 1 nfectious Bronchitis virus isolated from poultry flocks in Britain between 1981 and 1983. Av ian Pathology, 13: 733,7 41.
• JANE K.A. COOK and M.B. HUGGINS, 1986: Newly isolated serotypes of lnfectious Bronchitis virus: their role in disaese. Avian Pat.hology, 15: 128- 138.
• JANE K.A. COOK, 1988: lB virus serum neutralisation test in tracheal organ culture. HPRSjournal, 1: 1-2.
• K.OTSUKi, M.TSUBOKURA, 1981: Plaque formatian by avian lnfectious Bronchitis virus in primary chick embryo fibroblast cells in the presence of Trypsin. Archives of virology. 70 : 315-320.
• K. OTSUKi, T. NAKAMURA, Y.KAWAOKA and M.TSUBOKURA, 1987: lnterferon induction by Avian infectious Bronchitis virus in chickens . japenese journal Veterinary science, 44: 535-537.
• K. OTSUKi, Y. SAKAGAMi, T. NAKAMURA, N. KUBUTA, Y. KAWAOKA, M. TSUBOKURA, 1987: Comparison oftwo strains of Avian lnfectious Bronchitis virus fortheir interferon induction, viral growth and development of virus-neutralizing antibody in experimentally-infected chickens. Veterinary Microbiology, 15: 31-40.
• K. OTSUKi, Y. SAKAGAMi, M. TSUBOKURA, 1987: Serological relationship among ten strains of Avian lnfectious Bronchitis virus. Acta Virology, 31 : 138,145.
• M.S. Hofstad, 1984: lnfectious Bronchitis Disaese of poultry. 8. th .Edition. 429-443.
• PHYLAXIA Veterinary Research lnstutite, Hungary, 1989: lB aşı üretim laboratuvarı standart ve metodları.
• R.E.GOUGH, W.H. ALLAN and D. NEDELCiU, 1977: lmmune response to monovalent and bivalent Newcastle disaese and lnfectious Bronchitis inactivated vaccines. Avian Pathology. 6: 131-142.
• S.G. THAYER, C.S. EIDSON and S.H. KLEVEN, 1983: Multivalent inactivated virus oil emulsion vaccines in Broiler Breeder chickens. 1. Newcastle disaese virus and lnfectious Bronchitis virus bivalent vaccines. Poultry science 62: 1978-1983.
• WEYBRIDGE Veterinary Central Laboratory,UK, 1982:Compendium of Guidelines on the quality control of veterinary immunological products.1 :1-19.