Araştırma Makalesi
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Kanatlıların IBV-Enfeksiyöz Bronşitis Hastalığına Karşı H-120 ve H-52 Suşları ile Canlı, Liyofilize, Pilot Aşı Hazırlanması

Yıl 1995, Cilt: 8 Sayı: 1, 218 - 229, 01.12.1995

Öz

Bu araştırmada embriyolu SPF yumurtada liyofilize Mass. tipi H-120 ve H-
52 Enfeksiyöz Bronşitis virüs suşları ile aşı üretilip, kanatlıların bu hastalığa
karşı kullanılması amaçlanmıştır.
Canlı, liyofilize, Mass. tipi H-120 ve H-521BV suşları 9-11 günlük embriyolu
SPF yumurtaya corio :ıllantoik yolla in kule edilmiş, 36-48 saatler arası amnioallantoik
sıvı toplanmış , sterilite ve titre kontrolleri yapıldıktan sonra %2-3
oranında tindalize edilmiş süt tozu ilave edilmiş ve 1000 doz olacak şekilde
ampullere taksim edilerek liyofilize edilen ve -20 °C'Iik deep-freeze odalarda
saklanmaktadır. (20,23)
Aşılar şu anda istekler doğrultusunda +4 °C'de özel ambalajlarında tüm
yurda sevk edilmekte olup üretimimiz tüm ülkenin ihtiyacına cevap verecek
durumdadır.

Kaynakça

  • A.J . BROWN. C. D. BRACEWEL, 1985: Application of the haemagglutination inhibition test to typhing of infectious brochitis virus. Veterinary Record, 166: 47-48.
  • A.P.A. MOCKETT, JANE K.A. COOK and M.B. HUGGINS, 1987: Maternally- derivied antibody to lnfectious Bronchitis virus: lt's detection in chick trachea and serum and its role in protection. Avian Pathology, 16: 407-416.
  • A .. J.BROWN, C.D.BRACEWELL, 1988: Effect of repeated infections of chickens with lnfectious bronchitis viruses on the specifity of their antibody responses. Veterinary record . 122: 707-708.
  • C. PEJKOVSKI, F.G.DAVELAAR and B.KOUWENHOVEN, 1979: lmmunnosuppresive effect of lnfectious Bursa! disease virus on vaccination against infectious Bronchtis. Avian Pathology. 8:95-106.
  • D.J.ALEXANDER and N.J .CHETTLE, 1977: Procedures for the hacmagglutinasyon and the haemagglutination in h ibition testsfor avi an lnfections bronchitis virus. Avian Pathology, 6:9-17.
  • F.G. DAVELAAR, A.NOORDZIJ and J.A.VAN DER DONK, 1982: A study on the synthesis and seeretian of immunoglibulins by the harderian gland of the fowl after drop vaccination against lnfect ious Bronchitis at 1 dayold. Avian Pathology. 11 : 63,79.
  • GORDON and JORDON, 1984: lnfectious Bronchitis diaease. Poultry Disease, 2. nd. Edition, 164-172.
  • H. WILLIAMS SMITH, JANE K.A. COOK and ZÖE E.PARSELL, 1985: The experimantal infcction of chickens with mixtures of infectious Bronchitis virus and E.coli. J.Gen.Virol. 66: 777-786
  • HOUGHTON POULTRY RESEARCH STATION-HPRS, UK, 1987: lnfectious Bronchitis laboratuvarı standart ve metotları .
  • International Association of Biological Standardization, 1973: Report of the Avi an product standardization committe. 1 :12-15.
  • JANE K.A. COOK, J.H. DARBYSHIRE and R.W. PETIERS, 1976: The use of chicken tracheal organ cultures for the isolation and assay of Avi an lnfectious Bronchitis virus. Archives of Virology. 50: 109-118.
  • JANE K.A. COOK, 1984: The classification of new serotypes on 1 nfectious Bronchitis virus isolated from poultry flocks in Britain between 1981 and 1983. Av ian Pathology, 13: 733,7 41.
  • JANE K.A. COOK and M.B. HUGGINS, 1986: Newly isolated serotypes of lnfectious Bronchitis virus: their role in disaese. Avian Pat.hology, 15: 128- 138.
  • JANE K.A. COOK, 1988: lB virus serum neutralisation test in tracheal organ culture. HPRSjournal, 1: 1-2.
  • K.OTSUKi, M.TSUBOKURA, 1981: Plaque formatian by avian lnfectious Bronchitis virus in primary chick embryo fibroblast cells in the presence of Trypsin. Archives of virology. 70 : 315-320.
  • K. OTSUKi, T. NAKAMURA, Y.KAWAOKA and M.TSUBOKURA, 1987: lnterferon induction by Avian infectious Bronchitis virus in chickens . japenese journal Veterinary science, 44: 535-537.
  • K. OTSUKi, Y. SAKAGAMi, T. NAKAMURA, N. KUBUTA, Y. KAWAOKA, M. TSUBOKURA, 1987: Comparison oftwo strains of Avian lnfectious Bronchitis virus fortheir interferon induction, viral growth and development of virus-neutralizing antibody in experimentally-infected chickens. Veterinary Microbiology, 15: 31-40.
  • K. OTSUKi, Y. SAKAGAMi, M. TSUBOKURA, 1987: Serological relationship among ten strains of Avian lnfectious Bronchitis virus. Acta Virology, 31 : 138,145.
  • M.S. Hofstad, 1984: lnfectious Bronchitis Disaese of poultry. 8. th .Edition. 429-443.
  • PHYLAXIA Veterinary Research lnstutite, Hungary, 1989: lB aşı üretim laboratuvarı standart ve metodları.
  • R.E.GOUGH, W.H. ALLAN and D. NEDELCiU, 1977: lmmune response to monovalent and bivalent Newcastle disaese and lnfectious Bronchitis inactivated vaccines. Avian Pathology. 6: 131-142.
  • S.G. THAYER, C.S. EIDSON and S.H. KLEVEN, 1983: Multivalent inactivated virus oil emulsion vaccines in Broiler Breeder chickens. 1. Newcastle disaese virus and lnfectious Bronchitis virus bivalent vaccines. Poultry science 62: 1978-1983.
  • WEYBRIDGE Veterinary Central Laboratory,UK, 1982:Compendium of Guidelines on the quality control of veterinary immunological products.1 :1-19.

Preparation of live Lyophilized, pilot vaccine with H-52 strain against IBV Infections Bronchitis Disease of poultry

Yıl 1995, Cilt: 8 Sayı: 1, 218 - 229, 01.12.1995

Öz

The Study on live, Lyophilized IBV vaccines production by using IBV H-
120 and H-52 strains.
In this research; Vaccine production against to lnfections Bronchitis disease
had been aimed by the live, freeze-dried , Mass. type H-120 and H-52
IBV strains had been inoculated to 9-11 days old embriyoted SPF eggs by the
corio-allontoic route .After 36-48 hours, the virus content. 2-3 % tyndalizated
ski m med m ilk powder is ad d ed to vaccine harvest and lyophilized . Lyophilized
vaccines are stored in the -20 oc deep-freeze room . (20 ,23)
According to demands, the vaccines have been dispatching to all over the
country, in the special boxes at +4°C.

Kaynakça

  • A.J . BROWN. C. D. BRACEWEL, 1985: Application of the haemagglutination inhibition test to typhing of infectious brochitis virus. Veterinary Record, 166: 47-48.
  • A.P.A. MOCKETT, JANE K.A. COOK and M.B. HUGGINS, 1987: Maternally- derivied antibody to lnfectious Bronchitis virus: lt's detection in chick trachea and serum and its role in protection. Avian Pathology, 16: 407-416.
  • A .. J.BROWN, C.D.BRACEWELL, 1988: Effect of repeated infections of chickens with lnfectious bronchitis viruses on the specifity of their antibody responses. Veterinary record . 122: 707-708.
  • C. PEJKOVSKI, F.G.DAVELAAR and B.KOUWENHOVEN, 1979: lmmunnosuppresive effect of lnfectious Bursa! disease virus on vaccination against infectious Bronchtis. Avian Pathology. 8:95-106.
  • D.J.ALEXANDER and N.J .CHETTLE, 1977: Procedures for the hacmagglutinasyon and the haemagglutination in h ibition testsfor avi an lnfections bronchitis virus. Avian Pathology, 6:9-17.
  • F.G. DAVELAAR, A.NOORDZIJ and J.A.VAN DER DONK, 1982: A study on the synthesis and seeretian of immunoglibulins by the harderian gland of the fowl after drop vaccination against lnfect ious Bronchitis at 1 dayold. Avian Pathology. 11 : 63,79.
  • GORDON and JORDON, 1984: lnfectious Bronchitis diaease. Poultry Disease, 2. nd. Edition, 164-172.
  • H. WILLIAMS SMITH, JANE K.A. COOK and ZÖE E.PARSELL, 1985: The experimantal infcction of chickens with mixtures of infectious Bronchitis virus and E.coli. J.Gen.Virol. 66: 777-786
  • HOUGHTON POULTRY RESEARCH STATION-HPRS, UK, 1987: lnfectious Bronchitis laboratuvarı standart ve metotları .
  • International Association of Biological Standardization, 1973: Report of the Avi an product standardization committe. 1 :12-15.
  • JANE K.A. COOK, J.H. DARBYSHIRE and R.W. PETIERS, 1976: The use of chicken tracheal organ cultures for the isolation and assay of Avi an lnfectious Bronchitis virus. Archives of Virology. 50: 109-118.
  • JANE K.A. COOK, 1984: The classification of new serotypes on 1 nfectious Bronchitis virus isolated from poultry flocks in Britain between 1981 and 1983. Av ian Pathology, 13: 733,7 41.
  • JANE K.A. COOK and M.B. HUGGINS, 1986: Newly isolated serotypes of lnfectious Bronchitis virus: their role in disaese. Avian Pat.hology, 15: 128- 138.
  • JANE K.A. COOK, 1988: lB virus serum neutralisation test in tracheal organ culture. HPRSjournal, 1: 1-2.
  • K.OTSUKi, M.TSUBOKURA, 1981: Plaque formatian by avian lnfectious Bronchitis virus in primary chick embryo fibroblast cells in the presence of Trypsin. Archives of virology. 70 : 315-320.
  • K. OTSUKi, T. NAKAMURA, Y.KAWAOKA and M.TSUBOKURA, 1987: lnterferon induction by Avian infectious Bronchitis virus in chickens . japenese journal Veterinary science, 44: 535-537.
  • K. OTSUKi, Y. SAKAGAMi, T. NAKAMURA, N. KUBUTA, Y. KAWAOKA, M. TSUBOKURA, 1987: Comparison oftwo strains of Avian lnfectious Bronchitis virus fortheir interferon induction, viral growth and development of virus-neutralizing antibody in experimentally-infected chickens. Veterinary Microbiology, 15: 31-40.
  • K. OTSUKi, Y. SAKAGAMi, M. TSUBOKURA, 1987: Serological relationship among ten strains of Avian lnfectious Bronchitis virus. Acta Virology, 31 : 138,145.
  • M.S. Hofstad, 1984: lnfectious Bronchitis Disaese of poultry. 8. th .Edition. 429-443.
  • PHYLAXIA Veterinary Research lnstutite, Hungary, 1989: lB aşı üretim laboratuvarı standart ve metodları.
  • R.E.GOUGH, W.H. ALLAN and D. NEDELCiU, 1977: lmmune response to monovalent and bivalent Newcastle disaese and lnfectious Bronchitis inactivated vaccines. Avian Pathology. 6: 131-142.
  • S.G. THAYER, C.S. EIDSON and S.H. KLEVEN, 1983: Multivalent inactivated virus oil emulsion vaccines in Broiler Breeder chickens. 1. Newcastle disaese virus and lnfectious Bronchitis virus bivalent vaccines. Poultry science 62: 1978-1983.
  • WEYBRIDGE Veterinary Central Laboratory,UK, 1982:Compendium of Guidelines on the quality control of veterinary immunological products.1 :1-19.
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Cerrahi
Bölüm Araştırma Makaleleri
Yazarlar

Ragıp Bayraktar Bu kişi benim

Canan Tayhanı Bu kişi benim

M. Zafer Zoğ Bu kişi benim

Salih Çiçek Bu kişi benim

Yayımlanma Tarihi 1 Aralık 1995
Gönderilme Tarihi 1 Aralık 1995
Yayımlandığı Sayı Yıl 1995 Cilt: 8 Sayı: 1

Kaynak Göster

APA Bayraktar, R., Tayhanı, C., Zoğ, M. Z., Çiçek, S. (1995). Kanatlıların IBV-Enfeksiyöz Bronşitis Hastalığına Karşı H-120 ve H-52 Suşları ile Canlı, Liyofilize, Pilot Aşı Hazırlanması. Etlik Veteriner Mikrobiyoloji Dergisi, 8(1), 218-229.


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