The Effect of Chlorogenic Acid on 5-Fluorouracil-Induced Oxidative Damage in Rat Ovarian Tissue

Ahmet Mentese; Selim Demir; Nihal Türkmen; Yüksel Aliyazıcıoğlu; Orhan Değer

EN TR

The Effect of Chlorogenic Acid on 5-Fluorouracil-Induced Oxidative Damage in Rat Ovarian Tissue

Abstract

5-fluorouracil (5-FU) is a potent anticancer agent, but its significant tissue toxicity associated with increased oxidative stress and inflammation may limit its clinical use. Chlorogenic acid (CGA) is a dietary polyphenol found in a variety of foods and beverages, including coffee, apples, pears, strawberries, and grapes. It was aimed to evaluate the therapeutic effects of CGA on 5-FU-induced ovotoxicity through oxidative stress and inflammation parameters in this study. Thirty female Sprague-Dawley rats were divided into 5 groups with equal numbers of subjects (n=6): control, 5-FU (100 mg/kg), 5-FU+CGA (1.5 mg/kg), 5-FU+CGA (mg/kg) and CGA (3 mg/kg). The ovarian tissue levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), 8-hydroxy-2' -deoxyguanosine (8-OHdG), catalase (CAT) and interleukin-6 (IL-6) were determined using commercial spectrophotometric kits. While 5-FU treatment increased MDA, TOS, 8-OHdG and IL-6 levels in ovarian tissue, it significantly decreased TAS and CAT levels (p<0.05). These parameters, indicating 5-FU-induced toxicity, were significantly reversed with CGA administrations in a dose-dependent manner. The results support the view that CGA may be a useful modulator in attenuating 5-FU-induced ovotoxicity.

Keywords

5-fluorouracil , Chlorogenic acid , Inflammation , Ovotoxicity , Oxidative stress

Klorojenik Asidin Sıçan Yumurtalık Dokusunda 5-Florourasil ile İndüklenen Oksidatif Hasar Üzerine Etkisi

Öz

5-fluorourasil (5-FU) güçlü bir antikanser ajandır, ancak artan oksidatif stres ve inflamasyon ile ilişkili önemli doku toksisitesi klinik kullanımını sınırlayabilmektedir. Klorojenik asit (CGA) kahve, elma, armut, çilek ve üzüm dahil olmak üzere çeşitli yiyecek ve içeceklerde bulunan bir diyet polifenolüdür. Bu çalışmada, CGA'nın 5-FU ile indüklenen yumurtalık dokusu toksisitesi üzerine terapötik etkilerinin oksidatif stres ve inflamasyon parametreleri üzerinden değerlendirilmesi amaçlandı. Otuz Sprague-Dawley ırkı dişi sıçan eşit sayıda denek (n=6) içeren 5 gruba ayrıldı: kontrol, 5-FU (100 mg/kg), 5-FU+CGA (1.5 mg/kg), 5-FU+CGA (mg/kg) ve CGA (3 mg/kg). Yumurtalık dokularında malondialdehit (MDA), toplam oksidan durum (TOS), toplam antioksidan durum (TAS), 8-hidroksi-2'-deoksiguanozin (8-OHdG), katalaz (CAT) ve interlökin-6 (IL-6) seviyeleri ticari spektrofotometrik kitler kullanılarak belirlendi. 5-FU uygulaması yumurtalık dokusunda MDA, TOS, 8-OHdG ve IL-6 düzeylerini artırırken, TAS ve CAT düzeylerini anlamlı olarak azalttı (p<0.05). 5-FU ile indüklenen toksisiteyi gösteren bu parametreler, CGA tedavisi ile doza bağlı bir şekilde anlamlı derecede tersine çevrildi (p<0.05). Sonuçlar, CGA'nın 5-FU ile indüklenen yumurtalık dokusu toksisitesini azaltmada faydalı bir modülatör olabileceği görüşünü desteklemektedir.

Anahtar Kelimeler

5-florourasil , İnflamasyon , Klorojenik asit , Yumurtalık dokusu toksisitesi , Oksidatif stres

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Ahmet Mentese
0000-0003-2036-5317
Türkiye

Selim Demir ^*
0000-0002-1863-6280
Türkiye

Nihal Türkmen
0000-0002-8913-8692
Türkiye

Yüksel Aliyazıcıoğlu
0000-0001-9474-4307
Türkiye

Orhan Değer
0000-0003-3584-6324
Türkiye

Publication Date

December 30, 2022

Submission Date

November 6, 2022

Acceptance Date

November 21, 2022

Published in Issue

Year 2022 Volume: 1 Number: 1

IZ

https://izlik.org/JA87ND54MJ

APA

Mentese, A., Demir, S., Türkmen, N., Aliyazıcıoğlu, Y., & Değer, O. (2022). The Effect of Chlorogenic Acid on 5-Fluorouracil-Induced Oxidative Damage in Rat Ovarian Tissue. Farabi Tıp Dergisi, 1(1), 1-7. https://izlik.org/JA87ND54MJ

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The Effect of Chlorogenic Acid on 5-Fluorouracil-Induced Oxidative Damage in Rat Ovarian Tissue

Abstract

Keywords

Klorojenik Asidin Sıçan Yumurtalık Dokusunda 5-Florourasil ile İndüklenen Oksidatif Hasar Üzerine Etkisi

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

IZ

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