Şeker Pancarı Melası Kullanılarak Gluconacetobacter xylinum NRRL B-759 ile Bakteriyel Selüloz Üretimi Üzerine Etanolün Etkisi
Year 2017,
Volume: 29 Issue: 2, 31 - 36, 01.10.2017
Y. Emre Öz
Mehmet Kalender
Abstract
Selüloz dünyada en yaygın bulunan makromoleküler
yapılardan biridir ve genellikle bitkilerden elde edilir. Kâğıt kullanımının
artması ve tıp alanındaki bazı gelişmelerden dolayı günümüzde araştırmacılar
alternatif selüloz üretme yöntemlerine odaklanmışlardır. Bakteriyel selüloz
formül olarak bitkisel selüloz ile aynı olmasına rağmen su tutma kapasitesi,
polimerizasyon derecesi ve yüksek saflık gibi bazı özellikler bakımından
bitkisel selülozdan farklıdır. Bu çalışmada, besi yeri ve karbon kaynağı olarak
şeker pancarı melası kullanılarak Gluconacetobacter
xylinus NRRL B-759 tarafından
bakteriyel selüloz üretimi incelenmiştir. Bu amaçla, bakteriyel selüloz
üretiminde melas konsantrasyonunun etkisi (40-240 g/L) ve besi yerine etanol
ilavesinin etkisi (5-20 g/L) araştırılmıştır. Sonuç olarak melas
konsantrasyonunun artmasıyla 200 g/L konsantrasyona kadar bakteriyel selüloz
üretiminin arttığı ve daha sonra azaldığı görülmüştür. Etanol ilavesinin
yapıldığı çalışmalarda etanollü besi yerinde üretilen bakteriyel selüloz
miktarının etanolsüz ortama göre en çok 10 g/L etanol ilavesinde arttığı
görülmüştür.
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Year 2017,
Volume: 29 Issue: 2, 31 - 36, 01.10.2017
Y. Emre Öz
Mehmet Kalender
References
- 1. Czaja, W., Romanovicz, D. and Brown, R. M. (2004). Structural Investigations of Microbial Cellulose produced in Stationary and Agitated Culture, Cellulose, 11.
- 2. Dazzo, F. B., Napoli, C., Dazzo, F., & Hubbell, D. (1975). Production of cellulose microfibrils in Rhizobium Production of Cellulose Microfibrils by Rhizobium1, (September 2016).
- 3. Gupta, B. S. H. S. G. (2010). Production of bacterial cellulose from Enterobacter amnigenus GH-1 isolated from rotten apple, 1823–1828. doi:10.1007/s11274-010-0363-1
- 4. Phisalaphong, M., & Jatupaiboon, N. (2008). Biosynthesis and characterization of bacteria cellulose – chitosan film, 74, 482–488. doi:10.1016/j.carbpol.2008.04.004
- 5. Chawla, P. R., Bajaj, I. B., Survase, S. A., & Singhal, R. S. (2009). Microbial Cellulose : Fermentative Production and Applications, 47(2), 107–124.
- 6. Paximada, P., Tsouko, E., Kopsahelis, N., Koutinas, A. A., & Mandala, I. (2016). Food Hydrocolloids Bacterial cellulose as stabilizer of o / w emulsions. Food hydrocolloids, 53, 225–232. doi:10.1016/j.foodhyd.2014.12.003
- 7. Kurosumi, A., Sasaki, C., Yamashita, Y., & Nakamura, Y. (2009). Utilization of various fruit juices as carbon source for production of bacterial cellulose by Acetobacter xylinum NBRC 13693. Carbohydrate Polymers, 76(2), 333–335. doi:10.1016/j.carbpol.2008.11.009
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- 9. Chen, L. (2012). Biotransformation of wheat straw to bacterial cellulose and its mechanism, (October). doi:10.1016/j.biortech.2012.10.029
- 10. Guo, X., Cavka, A., Jönsson, L. J., & Hong, F. (2013). Comparison of methods for detoxification of spruce hydrolysate for bacterial cellulose production, 1–14.
- 11. Li, Z., Wang, L., Hua, J., Jia, S., Zhang, J., & Liu, H. (2015). Production of nano bacterial cellulose from waste water of candied jujube-processing industry using Acetobacter xylinum. Carbohydrate Polymers, 120, 115–119. doi:10.1016/j.carbpol.2014.11.061
- 12. Shoda, S. O. B. M. (2005). Production of bacterial cellulose by Acetobacter xylinum BPR2001 using molasses medium in a jar fermentor, 45–51. doi:10.1007/s00253-004-1723-2
- 13. Mohammadkazemi, F., Azin, M., & Ashori, A. (2015). Production of bacterial cellulose using different carbon sources and culture media. Carbohydrate Polymers, 117, 518–523. doi:10.1016/j.carbpol.2014.10.008
14. Esa, F., Tasirin, S. M., & Rahman, N. A. (2014). Overview of Bacterial Cellulose Production and Application. Italian Oral Surgery, 2, 113–119. doi:10.1016/j.aaspro.2014.11.017
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