Güç Testleri ile Farklı Yıllara ait Datura stramonium L. Tohumlarının Canlılıklarının Belirlenmesi

Abstract

Hızlandırılmış yaşlandırma testi birçok kültürü yapılan türün tohumlarında canlılığı belirlemek için kullanılan güç testlerinden biridir. Bu yöntemle tohumun su alımındaki fark, nemli bir ortama maruz kaldıklarında nem içeriğinde büyük bir değişikliğin meydana gelebilmesidir. Bu nedenle, tohumlarda hızlandırılmış yaşlandırma testi için tuz çözeltileri yerine su kullanımı gibi bazı alternatifler araştırılmıştır. Çalışma 2018 yılında Uşak Üniversitesi Ziraat ve Doğa Bilimleri Fakültesi Araştırma Laboratuvarı'nda (2006-2016) farklı yaşlardaki Datura stramonium L. türlerine ait 4 lot ile yürütülmüştür. Çalışmamızın amacı, yabancı ot tohumlarının fizyolojik potansiyelini değerlendirmek için hızlandırılmış yaşlandırma testinin metodolojisini incelemek ve doymuş/doymuş NaCl çözeltilerinin tohumlardan su alımını kontrol etmenin alternatifi olarak kullanma olasılığını doğrulamaktır. Bu amaçla kullandığımız testler; nem içeriği testi (fırın yöntemi), çimlenme ve fide çıkış testleri (25 ⁰C, 30 gün), hızlandırılmış yaşlandırma testi (kontrol/su), doymuş ve doymamış NaCl çözeltisi kullanılarak hızlandırılmış yaşlandırma (42 ° C, 24, 48, 72 ve 96 saat) ve EC testleridir (24, 48 ve 72 saat). Tohum nem içeriği yaklaşık % 3-7 arasında bulunmuştur. Genel olarak, 2011 ve 2016 tohumları yaşlandırma testlerinde iyi sonuçlar vermiş olup, bu durum yaşayabilirlik performansının farklı şekillerde karşılaştırılmasına olanak sağlamıştır. Ancak, test süreleri arasında anlamlı bir fark gözlenmemiştir. Fide çıkış testinde ise 2016 tohumları sonuç vermiştir. Sebze tohumlarında uygulanan bu alternatif güç testlerinin yabancı ot tohumlarında da test edilebileceği gözlenmiştir.

Keywords

• Datura stramonium L.,çimlenme kapasitesi,güç testleri,fide çıkışı

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