Investigation of The Effects of The Different Extraction Conditions on Phenolic Compounds Extraction from Onion Skin
Year 2024,
Volume: 13 Issue: 2, 90 - 100, 30.11.2024
Semra Topuz Türker
,
Mustafa Bayram
Abstract
Onion skin, which is a waste from the onion cultivation and processing industry, is one of the richest natural sources of phenolic compounds. In this study, the onion skin was extracted using different solvents and extraction times at various solid:solvent ratios to optimize the total phenolic compounds (TPC) and total flavonoid compounds (TFC) of the onion skin extract. The greatest content of TPC (61.29 mg GAE/g) and TFC (56.83 mg QE/g) of the extracts were obtained with 50% ethanol (ethanol/water; v/v) in 0.2 g/10 mL solid:solvent ratio. The highest TPC value was detected at the end of 90 minutes for 0.2 g/5 mL (55.25 mg GAE/g) and at the end of 120 minutes for 0.2 g/10 mL (60.14 mg GAE/g), while the highest TFC (51.06; 56.55 mg QE/g) value for both solid:solvent ratios were detected at the end of 120 minutes. It was found that the TPC and TFC of the onion skin were strongly influenced by the extraction condition. The findings of this study show that onion skin extract is a good source of phenolic compounds and emphasize that the extraction parameters used in obtaining onion skin extract are important.
Supporting Institution
Tokat Gaziosmanpaşa University Scientific Research Projects
References
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- Antony, A., Farid, M., 2022. Effect of temperatures on polyphenols during extraction. Applied Sciences, 12(4), 2107.
- Christova-Bagdassarian, V.L., Atanasova, M.S., Hristova, V.K., Ahmad, M.A., 2016. Soild-liquid extraction kinetics of total phenolic, total flavonoid, rutin and tannin contents in 50% ethanol extract of Cotinus coggygria. International Journal of Scientific & Engineering Research, 7(2), 1466-1472.
- Benito-Román, Ó., Blanco, B., Sanz, M.T., Beltrán, S., 2020. Subcritical water extraction of phenolic compounds from onion skin wastes (Allium cepa cv. Horcal): Effect of temperature and solvent properties. Antioxidants, 9(12), 1233.
- Cacace, J.E., Mazza, G., 2003. Optimization of extraction of anthocyanins from black currants with aqueous ethanol. Journal of Food Science, 68(1), 240-248.
- Çoklar, H., Akbulut, M., 2016. Alıç (Crataegus orientalis) meyvesinin antioksidan aktivitesi ve fenolik bileşiklerinin ekstraksiyonu üzerine farklı çözgenlerin etkisi. Derim, 33(2), 237-248.
- Gaafar, A.A., Salama, Z.A., 2013. Phenolic compounds from artichoke (Cynara scolymus L.) by-products and their antimicrobial activities. Journal of Biology, Agriculture and Healthcare, 3, 1-6.
- Garcia-Salas, P., Morales-Soto, A., Segura-Carretero, A., Fernández-Gutiérrez, A., 2010. Phenolic-compound-extraction systems for fruit and vegetable samples. Molecules, 15(12), 8813-8826.
- Ghenabzia, I., Hemmami, H., Amor, I.B., Zeghoud, S., Seghir, B. B., Hammoudi, R., 2023. Different methods of extraction of bioactive compounds and their effect on biological activity: A review. International Journal of Secondary Metabolite, 10(4), 469-494.
- Güldane, M., Cingöz, A., 2023. Extraction of bioactive compounds from yellow onion peels: Taguchi-SAW hybrid optimization. Turkish Journal of Agriculture-Food Science and Technology, 11(1), 2589-2594.
- Jang, M., Asnin, L., Nile, S.H., Keum, Y.S., Kim, H.Y., Park, S.W., 2013. Ultrasound‐assisted extraction of quercetin from onion solid wastes. International Journal of Food Science & Technology, 48(2), 246-252.
- Jiménez-Moreno, N., Volpe, F., Moler, J.A., Esparza, I., Ancín-Azpilicueta, C., 2019. Impact of extraction conditions on the phenolic composition and antioxidant capacity of grape stem extracts. Antioxidants, 8(12), 597.
- Jin, E.Y., Lim, S., oh Kim, S., Park, Y.S., Jang, J.K., Chung, M. S., Park, H., Shim, K.S., Choi, Y.J., 2011. Optimization of various extraction methods for quercetin from onion skin using response surface methodology. Food Science and Biotechnology, 20(6), 1727-1733.
- Kusumocahyo, S.P., Wijaya, S., Dewi, A.A.C., Rahmawati, D., Widiputri, D.I., 2020. Optimization of the extraction process of coffee pulp as a source of antioxidant. In IOP Conference Series: Earth and Environmental Science, IOP Publishing, 443(1), 012052.
- Kim, S.W., Ko, M.J., Chung, M.S., 2019. Extraction of the flavonol quercetin from onion waste by combined treatment with intense pulsed light and subcritical water extraction. Journal of Cleaner Production, 231, 1192-1199.
- Kumar, S., Pandey, A.K., 2013. Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013(1), 162750.
- Kumar, M., Barbhai, M.D., Hasan, M., Dhumal, S., Singh, S., Pandiselvam, R., Rais, N., Natta, S., Senapathy, M., Sinha, N., Amarowicz, R., 2022. Onion (Allium cepa L.) peel: A review on the extraction of bioactive compounds, its antioxidant potential, and its application as a functional food ingredient. Journal of Food Science, 87(10), 4289-4311.
- Luna-Guevara, M.L., Luna-Guevara, J.J., Hernández-Carranza, P., Ruíz-Espinosa, H., Ochoa-Velasco, C.E., 2018. Phenolic compounds: A good choice against chronic degenerative diseases. Studies in Natural Products Chemistry, 59, 79-108.
- Martino, K.G., Guyer, D., 2004. Supercritical fluid extraction of quercetin from onion skins. Journal of Food Process Engineering, 27(1), 17-28.
- Rahardhian, M.R.R., Murti, B.T., Wigati, D., Suharsanti, R., Putri, C.N., 2019. Solvent concentration effect on total flavonoid and total phenolic contents of Averrhoa bilimbi leaf extract. Pharmaciana, 9(1), 137-144.
- Sagar, N.A., Pareek, S., Gonzalez-Aguilar, G.A., 2020. Quantification of flavonoids, total phenols and antioxidant properties of onion skin: A comparative study of fifteen Indian cultivars. Journal of Food Science and Technology, 1-10.
- Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158.
- Škerget, M., Majhenič, L., Bezjak, M., Knez, Ž., 2009. Antioxidant, radical scavenging and antimicrobial activities of red onion (Allium cepa L.) skin and edible part extracts. Chemical and Biochemical Engineering Quarterly, 23(4), 435-444.
- Soquetta, M.B., Terra, L.D.M., Bastos, C.P., 2018. Green technologies for the extraction of bioactive compounds in fruits and vegetables. CyTA-Journal of Food, 16(1), 400-412.
- Stoica, F., Rațu, R.N., Veleșcu, I.D., Stănciuc, N., Râpeanu, G., 2023. A comprehensive review on bioactive compounds, health benefits, and potential food applications of onion (Allium cepa L.) skin waste. Trends in Food Science & Technology, 104173.
- Şahin, S., 2011. Obtaining of olive leaf extract with supercritical CO2 and investigation of the oleuropein content in the extract. Ph.D. Thesis, İstanbul University, Graduate School of Natural and Applied Sciences, p. 212, İstanbul-Turkey (in Turkish).
- Türkyılmaz, M., Tağı, Ş., Özkan, M., 2017. Narın farklı bölümlerinin polifenol içeriği, antioksidan ve antibakteriyel aktivitesi üzerine ekstraksiyon çözgenlerinin etkisi. Akademik Gıda, 15(2), 109-118.
- Viera, V.B., Piovesan, N., Rodrigues, J.B., de O Mello, R., Prestes, R.C., Dos Santos, R.C.V., De Vaucher, R.A., Hautrive, T.P., Kubota, E.H., 2017. Extraction of phenolic compounds and evaluation of the antioxidant and antimicrobial capacity of red onion skin (Allium cepa L.). International Food Research Journal, 24(3), 990-999.
- Yolci, M.S., Tunçtürk, R., Tunçtürk, M., 2022. Farklı ekstraksiyon çözücüleri ve hasat zamanlarının aspir (Carthamus tinctorius L.) çiçeklerinin toplam fenol ve flavonoid miktarları ile antioksidan aktivitesi üzerine etkileri. International Journal of Life Sciences and Biotechnology, 5(1), 97-109.
Soğan Kabuğundan Fenolik Bileşik Ekstraksiyonu Üzerine Farklı Ekstraksiyon Koşullarının Etkisinin Araştırılması
Year 2024,
Volume: 13 Issue: 2, 90 - 100, 30.11.2024
Semra Topuz Türker
,
Mustafa Bayram
Abstract
Soğan yetiştirme ve işleme endüstrisinin atıklarından biri olan soğan kabuğu, fenolik bileşiklerin en zengin doğal kaynaklarından biridir. Bu çalışmada, soğan kabuğu ekstraktındaki toplam fenolik madde (TFM) ve toplam flavonoid (TFL) değerlerini optimize etmek için soğan kabuğu farklı katı:çözücü oranlarında çeşitli çözücüler ve ekstraksiyon süreleri kullanılarak ekstrakte edilmiştir. En yüksek TFM (61.29 mg GAE/g) ve TFL (56.83 mg KE/g) içeriği 0.2 g/10 mL katı:çözücü oranında %50 etanol (etanol/su; h/h) kullanılarak elde edilen ekstraktta tespit edilmiştir. En yüksek TFM değeri 0.2 g/5 mL için (55.25 mg GAE/g) 90 dakika sonunda ve 0.2 g/10 mL için (60.14 mg GAE/g) 120 dakika sonunda belirlenirken, her iki katı:sıvı oranı için en yüksek TFL (51.06; 56.55 mg KE/g) değeri 120 dakika sonunda saptanmıştır. Soğan kabuğundan elde edilen ekstraktın TFM ve TFL değerlerinin ekstraksiyon koşullarından önemli düzeyde etkilendiği tespit edilmiştir. Bu çalışmanın bulguları, soğan kabuğu ekstraktının iyi bir fenolik bileşik kaynağı olduğunu göstermekte ve bu ekstraktın eldesinde kullanılan ekstraksiyon parametrelerinin önemli olduğunu vurgulamaktadır.
References
- Almeida, É.S., de Oliveira, D., Hotza, D., 2019. Properties and applications of Morinda citrifolia (noni): A review. Comprehensive Reviews in Food Science and Food Safety, 18(4), 883-909.
- Anonymous, 2021. https://www.fao.org/faostat/en/#data/QCL (Accessed 16.10.2024).
- Anonymous, 2023. Turkish Food Codex Communiqué on Extraction Solvents Used in the Production of Foodstuffs and Food Components https://kms.kaysis.gov.tr/Home/Kurum/24308110 (Accessed 16.10.2024).
- Antony, A., Farid, M., 2022. Effect of temperatures on polyphenols during extraction. Applied Sciences, 12(4), 2107.
- Christova-Bagdassarian, V.L., Atanasova, M.S., Hristova, V.K., Ahmad, M.A., 2016. Soild-liquid extraction kinetics of total phenolic, total flavonoid, rutin and tannin contents in 50% ethanol extract of Cotinus coggygria. International Journal of Scientific & Engineering Research, 7(2), 1466-1472.
- Benito-Román, Ó., Blanco, B., Sanz, M.T., Beltrán, S., 2020. Subcritical water extraction of phenolic compounds from onion skin wastes (Allium cepa cv. Horcal): Effect of temperature and solvent properties. Antioxidants, 9(12), 1233.
- Cacace, J.E., Mazza, G., 2003. Optimization of extraction of anthocyanins from black currants with aqueous ethanol. Journal of Food Science, 68(1), 240-248.
- Çoklar, H., Akbulut, M., 2016. Alıç (Crataegus orientalis) meyvesinin antioksidan aktivitesi ve fenolik bileşiklerinin ekstraksiyonu üzerine farklı çözgenlerin etkisi. Derim, 33(2), 237-248.
- Gaafar, A.A., Salama, Z.A., 2013. Phenolic compounds from artichoke (Cynara scolymus L.) by-products and their antimicrobial activities. Journal of Biology, Agriculture and Healthcare, 3, 1-6.
- Garcia-Salas, P., Morales-Soto, A., Segura-Carretero, A., Fernández-Gutiérrez, A., 2010. Phenolic-compound-extraction systems for fruit and vegetable samples. Molecules, 15(12), 8813-8826.
- Ghenabzia, I., Hemmami, H., Amor, I.B., Zeghoud, S., Seghir, B. B., Hammoudi, R., 2023. Different methods of extraction of bioactive compounds and their effect on biological activity: A review. International Journal of Secondary Metabolite, 10(4), 469-494.
- Güldane, M., Cingöz, A., 2023. Extraction of bioactive compounds from yellow onion peels: Taguchi-SAW hybrid optimization. Turkish Journal of Agriculture-Food Science and Technology, 11(1), 2589-2594.
- Jang, M., Asnin, L., Nile, S.H., Keum, Y.S., Kim, H.Y., Park, S.W., 2013. Ultrasound‐assisted extraction of quercetin from onion solid wastes. International Journal of Food Science & Technology, 48(2), 246-252.
- Jiménez-Moreno, N., Volpe, F., Moler, J.A., Esparza, I., Ancín-Azpilicueta, C., 2019. Impact of extraction conditions on the phenolic composition and antioxidant capacity of grape stem extracts. Antioxidants, 8(12), 597.
- Jin, E.Y., Lim, S., oh Kim, S., Park, Y.S., Jang, J.K., Chung, M. S., Park, H., Shim, K.S., Choi, Y.J., 2011. Optimization of various extraction methods for quercetin from onion skin using response surface methodology. Food Science and Biotechnology, 20(6), 1727-1733.
- Kusumocahyo, S.P., Wijaya, S., Dewi, A.A.C., Rahmawati, D., Widiputri, D.I., 2020. Optimization of the extraction process of coffee pulp as a source of antioxidant. In IOP Conference Series: Earth and Environmental Science, IOP Publishing, 443(1), 012052.
- Kim, S.W., Ko, M.J., Chung, M.S., 2019. Extraction of the flavonol quercetin from onion waste by combined treatment with intense pulsed light and subcritical water extraction. Journal of Cleaner Production, 231, 1192-1199.
- Kumar, S., Pandey, A.K., 2013. Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013(1), 162750.
- Kumar, M., Barbhai, M.D., Hasan, M., Dhumal, S., Singh, S., Pandiselvam, R., Rais, N., Natta, S., Senapathy, M., Sinha, N., Amarowicz, R., 2022. Onion (Allium cepa L.) peel: A review on the extraction of bioactive compounds, its antioxidant potential, and its application as a functional food ingredient. Journal of Food Science, 87(10), 4289-4311.
- Luna-Guevara, M.L., Luna-Guevara, J.J., Hernández-Carranza, P., Ruíz-Espinosa, H., Ochoa-Velasco, C.E., 2018. Phenolic compounds: A good choice against chronic degenerative diseases. Studies in Natural Products Chemistry, 59, 79-108.
- Martino, K.G., Guyer, D., 2004. Supercritical fluid extraction of quercetin from onion skins. Journal of Food Process Engineering, 27(1), 17-28.
- Rahardhian, M.R.R., Murti, B.T., Wigati, D., Suharsanti, R., Putri, C.N., 2019. Solvent concentration effect on total flavonoid and total phenolic contents of Averrhoa bilimbi leaf extract. Pharmaciana, 9(1), 137-144.
- Sagar, N.A., Pareek, S., Gonzalez-Aguilar, G.A., 2020. Quantification of flavonoids, total phenols and antioxidant properties of onion skin: A comparative study of fifteen Indian cultivars. Journal of Food Science and Technology, 1-10.
- Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158.
- Škerget, M., Majhenič, L., Bezjak, M., Knez, Ž., 2009. Antioxidant, radical scavenging and antimicrobial activities of red onion (Allium cepa L.) skin and edible part extracts. Chemical and Biochemical Engineering Quarterly, 23(4), 435-444.
- Soquetta, M.B., Terra, L.D.M., Bastos, C.P., 2018. Green technologies for the extraction of bioactive compounds in fruits and vegetables. CyTA-Journal of Food, 16(1), 400-412.
- Stoica, F., Rațu, R.N., Veleșcu, I.D., Stănciuc, N., Râpeanu, G., 2023. A comprehensive review on bioactive compounds, health benefits, and potential food applications of onion (Allium cepa L.) skin waste. Trends in Food Science & Technology, 104173.
- Şahin, S., 2011. Obtaining of olive leaf extract with supercritical CO2 and investigation of the oleuropein content in the extract. Ph.D. Thesis, İstanbul University, Graduate School of Natural and Applied Sciences, p. 212, İstanbul-Turkey (in Turkish).
- Türkyılmaz, M., Tağı, Ş., Özkan, M., 2017. Narın farklı bölümlerinin polifenol içeriği, antioksidan ve antibakteriyel aktivitesi üzerine ekstraksiyon çözgenlerinin etkisi. Akademik Gıda, 15(2), 109-118.
- Viera, V.B., Piovesan, N., Rodrigues, J.B., de O Mello, R., Prestes, R.C., Dos Santos, R.C.V., De Vaucher, R.A., Hautrive, T.P., Kubota, E.H., 2017. Extraction of phenolic compounds and evaluation of the antioxidant and antimicrobial capacity of red onion skin (Allium cepa L.). International Food Research Journal, 24(3), 990-999.
- Yolci, M.S., Tunçtürk, R., Tunçtürk, M., 2022. Farklı ekstraksiyon çözücüleri ve hasat zamanlarının aspir (Carthamus tinctorius L.) çiçeklerinin toplam fenol ve flavonoid miktarları ile antioksidan aktivitesi üzerine etkileri. International Journal of Life Sciences and Biotechnology, 5(1), 97-109.