Investigating of Effects of Metformin on Melanoma Skin Cancer

Year 2021, , 111 - 115, 30.06.2021

Harun Alp Hidayet Tutun , Levent Altıntaş , Halil Mahir Kaplan , Ergin Şingirik , İbrahim Kahraman

https://doi.org/10.15321/GenelTipDer.2021.295

Abstract

Aim: Apoptotic pathways are important for resistance to chemotherapeutics drugs and cancer development. Metformin is a safe, inexpensive and effective drug used to treat type 2 diabetes. Furthermore, although there is evidence of anticancer effects, the mechanisms by which metformin exerts this effect are unclear. If metformin is thought to be safe, cheap and effective drugs, the potentially beneficial effects of these drugs are of great importance. Therefore, it was aimed to investigate Bcl-2, kaspaz-3, bax, wee-1, AIF, grp78 and gadd153 levels in metformin treated skin cancer cells.

Materials and Methods: For this propose we prepared culture of skin cancer cells then these cells were treated with metformin and it was analyzed kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF protein levels by ELISA on these cells.

Result: Treatment of metformin increased of proapoptotic proteins (kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF) and decreased antiapoptotic protein (bcl-2).

Conclusion: In conclusion, our study showed that metformin has apoptotic effect on melanoma skin cancer.

Keywords

Metformin, Melanoma skin cancer, kaspase-3, bcl-2, wee 1

Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması

Abstract

Amaç: Kanserlerde kemoterapötiklere gösterilen dirençte ve kanser gelişiminde apoptozisin önemli rolü vardır. Metformin tip 2 diyabet tedavisinde kullanılan güvenli, ucuz ve etkili bir ilaçtır. Ayrıca, antikanser etkilerinin olduğuna dair kanıtlar olmasına rağmen metforminin bu etkiyi hangi mekanizmalar ile gerçekleştirdiği açık değildir. Bu nedenle, çalışmamızda cilt kanseri hücrelerine metformin uygulamasının Bcl-2, kaspaz-3, bax, wee-1, AIF, grp78 ve gadd153 düzeyleri üzerine etkileri araştırıldı.

Gereç ve Yöntem: İlgili protein düzeylerinin belirlenmesinde ELISA testleri kullanıldı.

Bulgular: Çalışmamızda metforminin cilt kanseri hücrelerinde apoptozis proteinlerini (kaspaz-3, wee 1, gadd153, grp78 ve AIF) arttırdığı ve antiapoptotik markırı (bcl-2) azalttığı belirlendi.

Sonuç: Sonuç olarak çalışmamız; metforminin melanom cilt kanseri üzerine apoptotik etkisinin olduğunu göstermiştir.

Keywords

Metformin, Melanom cilt kanseri, kaspaz-3, bcl-2, wee 1

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