Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması

Harun Alp; Hidayet Tutun; Levent Altıntaş; Halil Mahir Kaplan; Ergin Şingirik; İbrahim Kahraman

doi:10.15321/GenelTipDer.2021.295

EN TR

Investigating of Effects of Metformin on Melanoma Skin Cancer

Öz

Aim: Apoptotic pathways are important for resistance to chemotherapeutics drugs and cancer development. Metformin is a safe, inexpensive and effective drug used to treat type 2 diabetes. Furthermore, although there is evidence of anticancer effects, the mechanisms by which metformin exerts this effect are unclear. If metformin is thought to be safe, cheap and effective drugs, the potentially beneficial effects of these drugs are of great importance. Therefore, it was aimed to investigate Bcl-2, kaspaz-3, bax, wee-1, AIF, grp78 and gadd153 levels in metformin treated skin cancer cells. Materials and Methods: For this propose we prepared culture of skin cancer cells then these cells were treated with metformin and it was analyzed kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF protein levels by ELISA on these cells. Result: Treatment of metformin increased of proapoptotic proteins (kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF) and decreased antiapoptotic protein (bcl-2). Conclusion: In conclusion, our study showed that metformin has apoptotic effect on melanoma skin cancer.

Anahtar Kelimeler

Metformin, Melanoma skin cancer, kaspase-3, bcl-2, wee 1

Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması

Öz

Amaç: Kanserlerde kemoterapötiklere gösterilen dirençte ve kanser gelişiminde apoptozisin önemli rolü vardır. Metformin tip 2 diyabet tedavisinde kullanılan güvenli, ucuz ve etkili bir ilaçtır. Ayrıca, antikanser etkilerinin olduğuna dair kanıtlar olmasına rağmen metforminin bu etkiyi hangi mekanizmalar ile gerçekleştirdiği açık değildir. Bu nedenle, çalışmamızda cilt kanseri hücrelerine metformin uygulamasının Bcl-2, kaspaz-3, bax, wee-1, AIF, grp78 ve gadd153 düzeyleri üzerine etkileri araştırıldı. Gereç ve Yöntem: İlgili protein düzeylerinin belirlenmesinde ELISA testleri kullanıldı. Bulgular: Çalışmamızda metforminin cilt kanseri hücrelerinde apoptozis proteinlerini (kaspaz-3, wee 1, gadd153, grp78 ve AIF) arttırdığı ve antiapoptotik markırı (bcl-2) azalttığı belirlendi. Sonuç: Sonuç olarak çalışmamız; metforminin melanom cilt kanseri üzerine apoptotik etkisinin olduğunu göstermiştir.

Anahtar Kelimeler

Metformin, Melanom cilt kanseri, kaspaz-3, bcl-2, wee 1

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Harun Alp Bu kişi benim
Türkiye

Hidayet Tutun
Türkiye

Levent Altıntaş
Türkiye

Halil Mahir Kaplan
Türkiye

Ergin Şingirik
Türkiye

İbrahim Kahraman Bu kişi benim
Türkiye

Yayımlanma Tarihi

30 Haziran 2021

Gönderilme Tarihi

20 Eylül 2019

Kabul Tarihi

6 Temmuz 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 31 Sayı: 2

DOI

https://doi.org/10.15321/GenelTipDer.2021.295

IZ

https://izlik.org/JA52HE35CN

APA

Alp, H., Tutun, H., Altıntaş, L., Kaplan, H. M., Şingirik, E., & Kahraman, İ. (2021). Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması. Genel Tıp Dergisi, 31(2), 111-115. https://doi.org/10.15321/GenelTipDer.2021.295

AMA

1.Alp H, Tutun H, Altıntaş L, Kaplan HM, Şingirik E, Kahraman İ. Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması. Genel Tıp Derg. 2021;31(2):111-115. doi:10.15321/GenelTipDer.2021.295

Chicago

Alp, Harun, Hidayet Tutun, Levent Altıntaş, Halil Mahir Kaplan, Ergin Şingirik, ve İbrahim Kahraman. 2021. “Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması”. Genel Tıp Dergisi 31 (2): 111-15. https://doi.org/10.15321/GenelTipDer.2021.295.

EndNote

Alp H, Tutun H, Altıntaş L, Kaplan HM, Şingirik E, Kahraman İ (01 Haziran 2021) Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması. Genel Tıp Dergisi 31 2 111–115.

IEEE

[1]H. Alp, H. Tutun, L. Altıntaş, H. M. Kaplan, E. Şingirik, ve İ. Kahraman, “Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması”, Genel Tıp Derg, c. 31, sy 2, ss. 111–115, Haz. 2021, doi: 10.15321/GenelTipDer.2021.295.

ISNAD

Alp, Harun - Tutun, Hidayet - Altıntaş, Levent - Kaplan, Halil Mahir - Şingirik, Ergin - Kahraman, İbrahim. “Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması”. Genel Tıp Dergisi 31/2 (01 Haziran 2021): 111-115. https://doi.org/10.15321/GenelTipDer.2021.295.

JAMA

1.Alp H, Tutun H, Altıntaş L, Kaplan HM, Şingirik E, Kahraman İ. Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması. Genel Tıp Derg. 2021;31:111–115.

MLA

Alp, Harun, vd. “Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması”. Genel Tıp Dergisi, c. 31, sy 2, Haziran 2021, ss. 111-5, doi:10.15321/GenelTipDer.2021.295.

Vancouver

1.Harun Alp, Hidayet Tutun, Levent Altıntaş, Halil Mahir Kaplan, Ergin Şingirik, İbrahim Kahraman. Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması. Genel Tıp Derg. 01 Haziran 2021;31(2):111-5. doi:10.15321/GenelTipDer.2021.295

Genel Tıp Dergisi Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.

Investigating of Effects of Metformin on Melanoma Skin Cancer

Öz

Anahtar Kelimeler

Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster