IMPROVED EXTRACTION OF BIOACTIVE COMPOUNDS FROM THE POLLENS OF TYPHA DOMINGENSIS WITH SEQUENTIAL CONVENTIONAL AND ULTRASOUND TREATMENT

Abstract

Typha domingensis pollen is obtained from staminate flowers of plant that is edible. In this study, bioactive compounds from pollen were extracted using ethanol and methanol with conventional method. Further ultrasonication was applied to the samples to improve extraction efficiency. The extraction yield was higher with ethanol (26.3±0.14%) than with methanol (25.4±0.3%) and applying ultrasonication increased the yields significantly (P <0.05). Total phenolic compounds in ethanol and methanol extracts were determined as 9.83±0.48 and 9.71±0.55 mg GAE/g dry matter with the conventional method, and 11.76±0.64 and 12.74±0.37 mg GAE/g dry matter after ultrasonication, respectively. The flavonoid content with ethanol extraction was significantly higher than with methanol in both conventional and ultrasonication methods (P <0.05). Antioxidant activities using DPPH, ABTS, CUPRAC, and FRAP tests, were determined, and enhanced antioxidant capacities were observed after ultrasonication. The bioactive compounds were qualitatively analyzed using UV-VIS spectroscopy and FTIR which confirmed the presence of polyphenols.

Keywords

• Typha domingensis pollen
• extraction
• ultrasonication
• ekstraksiyon
• ultrasonikasyon
• fitokimyasal

TYPHA DOMINGENSIS POLENLERİNDEN BİYOAKTİF BİLEŞİKLERİN EKSTRAKSİYONUNDA GELENEKSEL VE ULTRASONİK YÖNTEMLERİN ARDIŞIK KULLANIMI

Abstract

Typha domingensis bitkisinin, erkek çiçeklerinden elde edilen polen, yenilebilir özelliktedir. Bu çalışmada, polenden biyoaktif bileşikler, etanol ve metanol kullanılarak geleneksel yöntemle ekstrakte edilmiştir. Ardından, ekstraksiyon verimliliğini artırmak için örneklere ultrasonikasyon uygulanmıştır. Etanol ile ekstraksiyon verimi (%26.3±0.14), metanole (%25.4±0.3) göre daha yüksek bulunmuş ve ultrasonikasyon uygulaması verimi önemli ölçüde artırmıştır (P <0.05). Etanol ve metanol ekstraklarında, toplam fenolik bileşik içerikleri, geleneksel yöntemle sırasıyla 9.83±0.48 ve 9.71±0.55 mg GAE/g, ultrasonikasyon uygulamasından sonra ise 11.76±0.64 ve 12.74±0.37 mg GAE/g kuru madde olarak belirlenmiştir. Etanol ekstraksiyonundaki flavonoid içeriği hem geleneksel hem de ultrasonikasyon yöntemlerinde metanolden önemli ölçüde yüksek olmuştur (P <0.05). Örneklerin antioksidan aktiviteleri DPPH, FRAP, ABTS ve CUPRAC yöntemleriyle belirlenmiş ve sonuçlar ultrasonikasyonun antioksidan kapasitelerini de arttırdığını göstermiştir. Biyoaktif bileşikler, UV-VIS spektroskopisi ve FTIR ile kalitatif olarak analiz edilmiş ve polifenollerin varlığı doğrulanmıştır.

Keywords

• Typha domingensis pollen
• ekstraksiyon
• ultrasonikasyon
• biyoaktif bileşikler

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