SORBUS AUCUPARIA VE SORBUS KUSNETZOVII'DEN ELDE EDİLEN ETANOL EKSTRAKTLARININ ANTİOKSİDAN KAPASİTESİ, FENOLİK BİLEŞİK İÇERİĞİ VE MİNERAL KOMPOZİSYONU

Year 2025, Volume: 50 Issue: 2, 223 - 234, 14.04.2025

Kübra Çinar Topçu , Pınar Anlar , Özlem Çakır , Abdurrahman Sefalı

https://izlik.org/JA79DB97PG

Abstract

Rosaceae familyasına ait Sorbus aucuparia L. ve Sorbus kusnetzovii Zinserl. meyvelerinin etanol ekstraktları, ultrasonik destekli ekstraksiyon yöntemi kullanılarak elde edildi. S. aucuparia ve S. kusnetzovii'nin toplam fenolik içerikleri sırasıyla 8.44±0.66 mg GAE/g ve 8.65±2.18 mg GAE/g iken, toplam flavonoid içerikleri sırasıyla 2.30±0.01 mg QE/g ve 2.67±0.13 mg QE/g idi. Meyvelerin DPPH değerleri 9.59±0.00 mg TE/g ve 9.28±0.05 mg TE/g, FRAP değerleri 9.51±0.42 mg TE/g ve 13.38±0.52 mg TE/g ve CUPRAC değerleri 13.43±0.01 mg TE/g ve 23.21±0.74 mg TE/g olarak ölçüldü. Ayrıca, her iki Sorbus türünün de zengin mineral içeriğine sahip olduğu bulundu. Bu nedenle, S. aucuparia ve S. kusnetzovii meyvelerinin güçlü antioksidanlar, bol miktarda fenolik bileşik ve değerli mineraller içerdiği sonucuna varılabilir. Ayrıca, fonksiyonel gıda olarak önemli potansiyele sahiptirler ve daha ileri klinik araştırmalar için güçlü adaylardır.

Keywords

Sorbus aucuparia L. , Sorbus kusnetzovii Zinserl. , antioksidan , fenolik , ICP-MS

ANTIOXIDANT CAPACITY, PHENOLIC COMPOUND CONTENT, AND MINERAL COMPOSITION OF ETHANOL EXTRACTS FROM SORBUS AUCUPARIA AND SORBUS KUSNETZOVII

https://izlik.org/JA79DB97PG

Abstract

Ethanol extracts of Sorbus aucuparia L. and Sorbus kusnetzovii Zinserl. fruits, belonging to the Rosaceae family, were obtained using an ultrasonic-assisted extraction method. The total phenolic contents of S. aucuparia and S. kusnetzovii were 8.44±0.66 mg GAE/g and 8.65±2.18 mg GAE/g, respectively, while their total flavonoid contents were 2.30±0.01 mg QE/g and 2.67±0.13 mg QE/g, respectively. The fruits’ DPPH values were measured as 9.59±0.00 mg TE/g and 9.28±0.05 mg TE/g, FRAP values as 9.51±0.42 mg TE/g and 13.38±0.52 mg TE/g, and CUPRAC values as 13.43±0.01 mg TE/g and 23.21±0.74 mg TE/g. Furthermore, both Sorbus species were found to have a rich mineral content. Therefore, it can be concluded that S. aucuparia and S. kusnetzovii fruits contain potent antioxidants, abundant phenolic compounds, and valuable minerals. Furthermore, they have significant potential as functional foods and are strong candidates for further clinical research.

Keywords

Sorbus aucuparia L. , Sorbus kusnetzovii Zinserl. , antioxidant , phenolic , ICP-MS

References

• Aimone, C., Gaudino, E.C., Brncic, M., Barba, F.J., Grillo, G., Cravotto, G. (2024). Sorbus spp. berries extraction in subcritical water: Bioactives recovery and antioxidant activity. Applied Food Research, 4: 100391. https://doi.org/ 10.1016/j.afres.2024.100391.
• Aksoy, K.P. (2013). Ecological, morphological and anotomical study of Sorbus L. (Rosaceae) growing in province of Rize. Recep Tayyip Erdoğan University, MS Thesis, Rize.
• Akyıldırım Beğen, H., Eminağaoğlu, Ö. (2022). Taxonomic contributions to the Turkey Rosaceae family with new genera (Aria, Hedlundia, Torminalis). Turkish Journal of Biodiversity, 5(1): 36-49. https://doi.org/10.38059/ biodiversity.1090331.
• AlAli, M., Alqubaisy, M., Aljaafari, M.N., AlAli, A.O., Baqais, L., Molouki, A., Abushelaibi, A., Lai, K-S., Lim S-H.E. (2021). Nutraceuticals: Transformation of conventional foods into health promoters/disease preventers and safety considerations. Molecules, 26: 2540. https://doi.org/10.3390/molecules26092540.
• Apak, R., Güçlü, K., Özyürek, M., Bektaşoğlu, B., Çelik, S.E. (2009). A new method for determination of antioxidant capacity of foods: CUPRAC method, Flora Final Meeting, Istanbul Technical University, Istanbul, 25-26 May. Aslantas, R., Pırlak, L., Güleryüz, M. (2007). The nutritional value of wild fruits from the northeastern Anatolia region of Turkey. Asian Journal of Chemistry, 19(4): 3072-3078.
• Aurori, M., Niculae, M., Hanganu, D., Pall, E., Cenariu, M., Vodnar, D.C., Fit, N., Andrei, S. (2024). The antioxidant, antibacterial and cell-protective properties of bioactive compounds extracted from rowanberry (Sorbus aucuparia L.) fruits in vitro. Plants, 13: 538. https://doi.org/ 10.3390/plants13040538.
• Aurori, M., Andrei, S., Dreanca, A.I., Morohoschi, A.G., Cotul, M., Niculae, M., Nan, M.I., Codea, A. R., Gal, A.F. (2023). The nephroprotective effect of cornelian cherry (Cornus mas L.) and rowanberry (Sorbus aucuparia L.) in gentamicin-induced nephrotoxicity on wistar rats with emphasis on the evaluation of novel renal biomarkers and the antioxidant capacity in correlation with nitro-oxidative stress. Nutrients, 15: 4392. https://doi.org/10.3390/ nu15204392.
• Bánki, O., Roskov, Y., Döring, M., Ower, G., Vandepitte, L., Hobern, D., Remsen, D., Schal, P., DeWalt, R.E., Keping, M., Miller, J., Orrell, T., Aalbu, R., Adlar, R., Adriaenssens, E.M., Aedo, C., Aescht, E., Akkari, N., Alfenas-Zerbini, P. (2022). Catalogue of Life Checklist (Version 2022-02-18). Catalogue of Life. https://doi.org/ 10.48580/dfp4.
• Bao, J., Cai, Y., Sun, M., Wang, G., Corke, H. (2005). Anthocyanins, flavonols, and free radical scavenging activity of Chinese Bayberry (Myrica rubra) extracts and their color properties and stability. Journal of Agricultural and Food Chemistry, 53: 2327-2332.
• Beya, M.M., Netzel, M.E., Sultanbawa, Y., Smyth, H., Hoffman, L.C. (2021). Plant-based phenolic molecules as natural preservatives in comminuted meats: A review. Antioxidants, 10: 263. https://doi.org/10.3390/antiox10020263.
• Bobinaitė, R., Grootaert, C., Camp, J.V., Šarkinas, A., Liaudanskas, M., Žvikas, V., Viškelis, P., Venskutonis, P.R. (2020). Chemical composition, antioxidant, antimicrobial and antiproliferative activities of the extracts isolated from the pomace of rowanberry (Sorbus aucuparia L.). Food Research International, 136: 109310. https://doi.org/ 10.1016/j.foodres.2020.109310.
• Bozhuyuk, M.R. (2021). Morphological and biochemical diversity in fruits of rowanberry (Sorbus aucuparia L.) genotypes. Erwerbs-Obstbau, 63: 431-435. https://doi.org/10.1007/s10341-021-00603-4.
• Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M.H., ElSohly, M.A., Khan, I.A. (2014). Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: a comparative study. Evidence-Based Complementary and Alternative Medicine, 253875.
• Davis, P.H. (1965-85). Flora of Turkey and the East Aegean Islands, 1-9. Edinburgh Univ. Press, Edinburgh. Davis, P.H., Miller R.R., Tan K. (1988). Flora of Turkey and the East Aegean Islands, 10. Edinburgh Univ. Press, Edinburgh.
• Dorman, H.J.D., Peltoketo, A., Hiltunen, R., Tikkanen, M.J. (2003). Characterisation of the antioxidant properties of deodorised aqueous extracts from selected Lamiaceae herbs. Food Chemistry, 83: 255-262.
• Dönmez, A.A., Uğurlu Aydın, A., Tuna, M. (2022). A new species from Turkey, Sorbus erzincanica (Rosaceae); inference from genomic data and morphometry. Kew Bulletin, 77: 787-798. doi: 10.1007/S12225-022-10021-8.
• Ekin, H.N., Gokbulut, A., Ugurlu Aydin, Z., Donmez, A.A., Orhan, I.E. (2016). Insight into anticholinesterase and antioxidant potential of thirty-four Rosaceae samples and phenolic characterization of the active extracts by HPLC. Industrial Crops and Products, 91: 104-113. https://doi.org/10.1016/j.indcrop.2016.06.029.
• Eremeevaa, N.B., Makarova, N.V. (2022). Study of the content of antioxidants and their activity in concentrated extracts of cranberry (Vaccinium oxycoccus), sea buckthorn (Hippophae rhamnoides L.), blackberry (Rubus fruticosus), guelder rose (Viburnum opulus L.) and mountain ash (Sorbus aucuparia L.). Russian Journal of Bioorganic Chemistry, 48(7): 1392-1398.
• García-Valencia, S., Soto-Simental, S., Ocampo-López, J., Ayala-Martínez, M. (2022). Diet supplementation with dalbergia palo-escrito hexane extract in fattening rabbits: its effect on productive performance, carcass traits, meat characteristics and meatballs shelf-life. Italian Journal of Animal Science, 21(1): 1533-1542.
• Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (2012). (edlr.), List of Turkish Plants (Vascular Plants). Nezahat Gökyiğit Botanical Garden and Flora Research Association Publication. Istanbul.
• Güner, A., Özhatay, N., Ekim, T., Başer, K.H.C. (2000). Flora of Turkey and the East Aegean Islands 11, Edinburgh University Press, Edinburgh.
• Ibrahimov, A.M., Matsyura, A.V. (2018). New species of Sorbus (Rosaceae) for the flora of the Nakhchivan Autonomous Republic (Azerbaijan). Biosystems Diversity, 26(2): 92-97. doi: 10.15421/011814.
• Kocaimamoğlu, F. (2014). Elucidation of phenolic compounds of Sorbus aucuparia and Sorbus caucasica var. yaltirikii fruits by HPLC-UV. Recep Tayyip Erdoğan University, MS Thesis, Rize.
• Kremer, D., Müller, I.D., Stabentheiner, E., Vitali, D., Kopričane, M., Ruščić, M., Kosalec, I., Bezić, N., Dunkić, V. (2012). Phytochemical and micromorphological traits of endemic Micromeria pseudocroatica (Lamiaceae). Natural Product Communications, 7(12): 1667-1670.
• Krzywicka, M., Kobus, Z. (2023). Effect of the shape of ultrasonic vessels on the chemical properties of extracts from the fruit of Sorbus aucuparia. Applied Science, 13: 7805. https://doi.org/10.3390/app13137805.
• Lykholat, Y.L., Didur, O.O., Khromykh, N.O., Davydov, V.R., Borodai, Y.S., Kravchuk, K. V., Lykholat, T.Y. (2021). Comparative analysis of the antioxidant capacity and secondary metabolites accumulation in the fruits of rowan (Sorbus aucuparia L.) and some closely related species. Ecology and Noospherology, 32(1): 3-8. https://doi.org/10.15421/032101.
• Mayda, N., Özkök, A., Bayram, N.E., Gerçek, Y.C., Sorkun, K. (2020). Bee bread and bee pollen of different plant sources: Determination of phenolic content, antioxidant activity, fatty acid, and element profiles. Journal of Food Measurement and Characterization, 14: 1795-1809. https://doi.org/10.1007/s11694-020-00427-y.
• McDonald, S., Prenzler, P.D., Antolovich, M., Robards, K. (2001). Phenolic content and antioxidant activity of olive extracts. Food Chemistry, 73(1): 73-84.
• Meng, J., Fang, Y., Zhang, A., Chen, S., Xu, T., Ren, Z., Han, G., Liu, J., Li, H., Zhang, Z., Wang, H. (2011). Phenolic content and antioxidant capacity of Chinese raisins produced in Xinjiang Province. Food Research International, 44(9): 2830-2836.
• Mishra, B.K., Rastogi, A., Shukla, S. (2012). Regulatory role of mineral elements in the metabolism of medicinal plants. Medicinal and Aromatic Plant Science and Biotechnology, 1-23.
• Mostafa, H.B., Azab, E.F.E. (2022). Efficacy of green coffee as an antioxidant in beef meatballs compared with ascorbic acid. Food Chemistry: X, 14: 100336.
• Odunkıran, A. (2016). Chemical and physical properties of Sorbus fruit (Sorbus aucuparia L.) grown in Yusufeli town. Atatürk University, MS Thesis, Erzurum.
• Olszewska, M.A., Michel, P. (2009). Antioxidant activity of inflorescences, leaves and fruits of three Sorbus species in relation to their polyphenolic composition. Natural Product Research, 23(16): 1507-1521.
• Öz Atasever., Ö., Gerçekcioğlu, R. (2013). Some vegetative characteristics of service tree (sorbus domestica l.) genotypes, selected from Tokat ecology. Research Journal of Agricultural Sciences, 6(2): 97-101.
• Pereira, C., Barros L., SantosBuelga, C., Prieto, M.A. (2022). Editorial: Natural bioactives used as additives in food applications. Frontiers in Nutrition, 9: 1063942. doi: 10.3389/ fnut.2022.1063942.
• Perron, N.R., Brumaghim, J.L. (2009). A Review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys, 53: 75-100.
• Petkova, N.T., Ognyanov, M.H., Vrancheva, R.Z., Zhelev, P. (2020). Phytochemical, nutritional and antioxidant characteristics of whitebeam (Sorbus aria) fruits. Acta Scientiarum Polonorum, Technologia Alimentaria, 19(2): 219-229. http://dx.doi.org/10.17306/J.AFS.2020.0786.
• Raspe, O., Findlayt, C., Jacquemart, A-L. (2000). Sorbus aucuparia L. Journal of Ecology, 88: 910-930.
• Raudonis, R., Raudonė, L., Gaivelytė, K., Viškelis, P., Janulis, V. (2014). Phenolic and antioxidant profiles of rowan (Sorbus L.) fruits. Natural Product Research, 28(16): 1231-1240, http://dx.doi.org/ 10.1080/14786419.2014.895727.
• Rutkowska, M., Owczarek-Januszkiewicz, A., Magiera A., Gieleta M., Olszewska M.A. (2023). Chemometrics-driven variability evaluation of phenolic composition, antioxidant capacity, and-glucosidase inhibition of Sorbus aucuparia L. fruits from Poland: Identification of variability markers for plant material valorization. Antioxidants, 12: 1967. https://doi.org/10.3390/antiox12111967.
• Ryszczynska, S., Gumulak-Wołoszyn, N., Urbaniak, M., Stepien L., Bryła, M., Twaruzek, M., Waskiewicz, A. (2024). Inhibitory effect of Sorbus aucuparia extracts on the Fusarium proliferatum and F. culmorum growth and mycotoxin biosynthesis. Molecules, 29: 4257. https://doi.org/ 10.3390/molecules29174257.
• Sanchez-Moreno, C. (2002). Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems, Food Science and Technology International, 8: 121-127.
• Sarv, V., Venskutonis, P.R., Rätsep, R., Aluvee, A., Kazernaviciute, R., Bhat, R. (2021). Antioxidants characterization of the fruit, juice, and pomace of sweet rowanberry (Sorbus aucuparia L.) cultivated in Estonia. Antioxidants, 10: 1779. https://doi.org/10.3390/antiox10111779.
• Sefalı, A. (2023). Determination of wild fruits growing in Bayburt province and some examinations. Osmaniye Korkut Ata University Journal of Natural and Applied Sciences, 6 (1): 52-67.
• Shin, M-Y., Cho, Y-E., Park, C., Sohn, H-Y., Lim, J-H., Kwun, I-S. (2012). The supplementation of yam powder products can give the nutritional benefits of the antioxidant mineral (Cu, Zn, Mn, Fe and Se) intakes. Preventive Nutrition and Food Science, 17: 299-305. http://dx.doi.org/10.3746/ pnf.2012.17.4.299.
• Singh, P., Mijakovic, I. (2022). Rowan berries: A potential source for green synthesis of extremely monodisperse gold and silver nanoparticles and their antimicrobial property. Pharmaceutics, 14: 82. https://doi.org/10.3390/pharmaceutics14010082.
• Soetan, K.O., Olaiya, C.O., Oyewole, O.E. (2010). The importance of mineral elements for humans, domestic animals and plants: A review. African Journal of Food Science, 4(5): 200-222. http://www.academicjournals.org/ajfs.
• Sulimanec, A., Kragić, K., Sekovanić, A., Jurasović, J., Krbavčić, I. P., Vahčić, N., Vidaković, A., Poljak, I., Samarin, I.R. (2023). Chemical characterization and antioxidant potential of the rowan (Sorbus aucuparia L.) fruits from Alpine-Dinaric region of Croatia. Food Technology and Biotechnology, 61(4): 465-474. https://doi.org/10.17113/ftb.61.04.23.8225.
• Şen, M. (2011). Antioxidant Activity of Sorbus Fruits. MS Thesis, Istanbul University, Istanbul.
• Upadhyay, N.K., Kumar, M.Y. Gupta, A. (2010). Antioxidant, cytoprotective, and antibacterial effects of Sea buckthorn (Hippophae rhamnoides L.) leaves. Food Chemical Toxicology, 48(12): 3443-3448.
• Wang, C., An, X., Gao, Z., Li, Z., Tıan, S., Lu, Y. (2022). Effects of ethanolic extract from onion skin on the quality characteristics of beef patties during refrigerated storage. Journal of Food Science and Technology, 42: e118121. WHO, Guidelines for Assessment of Herbal Medicines With Reference to Contaminants and Residues, 2007. https://www.who.int/ publications/i/item/9789241594448.
• Zhou, S., Fang, Z., Lü, Y., Chen, J., Liu, D., Ye, X. (2009). Phenolics and antioxidant properties of bayberry (Myrica rubra Sieb. et Zucc.) pomace. Food Chemistry, 112: 394-399.