FARKLI KAYNAKLARDAN İZOLE EDİLMİŞ LAKTİK ASİT BAKTERİLERİNİN EKZOPOLİSAKKARİT ÜRETİMİ VE KOLESTEROL ASİMİLASYON YETENEKLERİNİN BELİRLENMESİ

Abstract

Bağırsak sistemimizde yer alan yararlı mikroorganizmaların önemli özelliklerinden birisi gastrointestinal sistemdeki farklı koşullara karşı direnç gösterebilmeleridir. Bu koşullar arasında safra tuzlarına karşı direnç ve düşük pH’da gelişim yer almaktadır. Gastrointestinal sistemde canlılıklarını sürdürebilmek için yararlı bakteriler spesifik enzim sentezi veya ekzopolisakkarit üretimi gibi çeşitli savunma mekanizmaları geliştirmişlerdir. Bu çalışmada çeşitli fermente gıdalardan ve bitkilerden izole edilmiş laktik asit bakterilerinin safra tuzu dirençleri, düşük pH’da gelişim özelliklerinin yanı sıra, ekzopolisakkarit üretimleri ve kolesterol asimilasyon yetenekleri belirlenmiştir. Ekzopolisakkarit (EPS) üretimlerinin 11.9 ile 1.1 mg/L arasında değiştiği belirlenirken toplam kolesterol asimilasyon yüzdeleri % 60.71 ile % 16.71 arasında değişim göstermiştir. Kolesterol asimilasyon yetenekleri ile EPS üretimleri arasında bir bağlantı bulunamamıştır. 

Keywords

• Kolesterol Asimilasyonu,Ekzopolisakkarit,düşük pH,safra tuzu direnci

DETERMINATION OF EXCOPOLISACCHARIDE PRODUCTION AND CHOLESTEROL ASSIMILATION ABILITIES OF LACTIC ACID BACTERIA ISOLATED FROM DIFFERENT SOURCES

Abstract

One of the most important features of the intestinal flora is resistance to many conditions in the gastrointestinal tract. These include resistance to bile salt and growth at low pH. Bacteria have developed various defense mechanisms, such as specific enzymes, exopolysaccharide production, against Gastrointestinal system, In our study, strains of lactic acid bacteria  isolated from various fermented foods and plants and all the strains were determined of their bile salt and low pH resistance. The strains were also tested for their exopolysaccharide production and total cholesterol assimilation properties. EPS production was observed at rations between 11.9 to 1.1 mg/L, and total cholesterol assimilation percentages ranged from 60.71 to 16.71. There was no relevance between cholesterol assimilation ability and their EPS production.

Keywords

• Cholesterol assimilation,low pH,exopolysaccharide,bile salt resistance

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