ADAÇAYI UÇUCU YAĞ ELDESİNDEN SONRA ARTA KALAN ÜRÜNLERİN BİYOAKTİF POTANSİYELLERİNİN BELİRLENMESİ

Abstract

Adaçayı (Salvia officinalis L.), bitkisel çay, baharat ve gıda tatlandırıcısı olarak kullanılan, çok sayıda biyoaktif bileşen içeren, Lamiaceae familyasından önemli bir tıbbi bitkidir. Bu araştırmada,  hidrodistilasyon ile adaçayı uçucu yağları uzaklaştırılıp, arta kalan sulu kısım ve atık yaprakların bileşimi incelenmiştir. Kurutulmuş adaçayı yaprakları 1 ve 2 saat olarak hidrodistilasyon yöntemi ile ekstrakte edilip arta kalan yapraklar tekrar kurutularak sırasıyla etil asetat ve etanol çözgenleri ile Soxhlet ekstraksiyonuna tabi tutulmuştur. Elde edilen sulu (Hidrosol1, Hidrosol2), etanollü (Etanol1, Etanol2) ve etil asetatlı (Etilasetat1, Etilasetat2) ekstraktların toplam fenolik ve flavonoid içerikleri, α-glukozidaz inhibisyon aktiviteleri, DPPH, ABTS ve β-karoten ağartma metotları kullanılarak antioksidan aktiviteleri kolorimetrik olarak belirlenmiştir. Test edilen ekstraktlar arasında toplam fenolik madde miktarı (60.63 mg GAE/g), toplam flavonoid miktarı (40.23 mg KE/g), radikal süpürme gücü ((DPPH (95.00 mg TEAC/g) ve ABTS (92.00 mg TEAC/g)) açısından en yüksek değer 2 saat hidrodistilasyon sonucu elde edilen Hidrosol2 örneğine ait bulunurken en yüksek antidiyabetik aktivitenin IC₅₀ (1.32) Etil asetat1 örneğine ait olduğu belirlenmiştir. 

Keywords

• Adaçayı,Ekstraksiyon,Fenolik,Antioksidan,Antidiyabetik

DETERMINATION OF THE BIOACTIVE POTENTIALS OF SAGE (SALVIA OFFICINALIS L.) BY PRODUCT AFTER REMOVAL OF ESSENTIAL OIL

Abstract

Sage (Salvia officinalis L.) is an important medicinal plant of the Lamiaceae family, which is contains many bioactive components and is used as herbal tea, spice and food seasoning. In this study, the essential oils of sage were removed by hydrodistilation and the composition of the remaining residual floral water and waste leaves were investigated. Dried sage leaves were extracted with hydrodistillation method for 1 and 2 hours and the remaining leaves were dried again and subjected to Soxhlet extraction first with ethyl acetate and then ethanol. Total phenolic, flavonoid contents, α-glucosidase inhibition activities and antioxidant activities by using DPPH, ABTS, and β-carotene methods in aqueous (Hydrosol1, Hydrosol2), ethanolic (Ethanol1, Ethanol2) and ethyl acetate (Ethyl acetate1, Ethyl acetate2)  extracts were determined by colorimetric methods. Among the different extracts tested, Hydrosol2, obtained from 2 h distillation, displayed the highest scores in terms of the total amount of phenolics (60.63 mg GAE/g), the total flavonoid content (40.23 mg CE/g), radical scavenging power ((DPPH (95.00 mg TEAC/g) and ABTS (92.00 mg TEAC/g)), while the highest antidiabetic activity (as IC₅₀ value) was determined in Ethyl acetate1 sample as 1.32.

Keywords

• Sage,extraction,phenolic,antioxidant,antidiabetic

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