THE EFFECT OF REGIONAL DIFFERENCE AND STORAGE TIME ON ANTIOXIDANT PROPERTIES OF PROPOLIS

Abstract

Propolis is a mixture of many other bioactive substances such as phenolic and flavonoid compounds, terpenes and tocopherol used in traditional medicine. The antioxidant-oxidant parameter measurements of propolis, obtained from Apis Mellifera Caucasica bee species in three different regions, were performed in In June and at the end of 8 months room temperature storage process in February with 1,1-diphenyl-2-picrilhydrazil (DPPH) and high performance liquid chromatography (HPLC). Vitamin B1 (44.24±5.04 - 64.48±5.12 μg / g), vitamin B2 (66.13±2.44 - 104.42±3.85 μg / g), vitamin A (3.58±0.09 - 17.44±0.25 μg / g), vitamin E (15.88±1.38 - 38.64±2.75 μg / g), malondialdehyde (MDA) (0.381±0.020 - 1.419±0.023 nmol / mL) and DPPH (95.42±1.22 - 368.22±4.31 mg/L) levels were observed. Results were accepted as mean±standard deviation and P <0.05 significance level. Propolis obtained from high altitude regions showed the best antioxidant properties in June analysis (P <0.05). Antioxidant levels are thought to vary in propolis samples depending on shelf life, climate and environmental conditions.

Keywords

• Antioxidant,storage,malondialdehyde,propolis,vitamins

BÖLGESEL FARKLILIK VE DEPOLAMA SÜRESİNİN PROPOLİSİN ANTİOKSİDAN ÖZELLİKLERİ ÜZERİNE ETKİSİ

Abstract

Propolis, geleneksel tıpta kullanılan fenolik ve flavonoid bileşikler, terpenler ve tokoferol gibi daha birçok biyoaktif madde içeren bir karışımdır. Üç farklı bölgede Apis Mellifera Caucasica arı türlerinden toplanan propolis örnekleri, Haziran ayında ve 8 ay oda sıcaklığında depolama süreci sonunda Şubat ayında 1,1-difenil-2-pikrilhidrazil (DPPH) ve yüksek performanslı sıvı kromatografisi (HPLC) ile antioksidan-oksidan parametre ölçümleri yapılmıştır. B1 vitamini (44.24±5.04 - 64.48±5.12 μg/g), B2 vitamini (66.13±2.44 - 104.42±3.85 μg/g), A vitamini (3.58±0.09 - 17.44±025 μg/g), E vitamini (15.88±1.38 - 38.64±2.75 μg/g), malondialdehit (MDA) (0.381±0.020 - 1.419±0.023 nmol/mL) ve DPPH (95.42±1.22 - 368.22±4.31 mg/L) düzeyleri gözlemlenmiştir. Sonuçlar ortalama±standart sapma ve P <0.05 anlamlılık düzeyi olarak kabul edilmiştir. Yüksek rakımlı bölgelerde elde edilen propolisin, Haziran ayında yapılan analizinde en iyi antioksidan özellik gösterdiği tespit edilmiştir (P <0.05). Raf ömrü, iklim ve çevresel koşullara bağlı olarak propolis örneklerinde antioksidan düzeylerin değişiklik gösterdiği düşünülmektedir. 

Keywords

• Antioksidan,HPLC,Malondialdehit,Vitaminler,propolis

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