GIDA KAYNAKLI PATOJEN ESCHERICHIA COLI O157:H7’YE KARŞI ETKİLİ BAKTERİYOFAJLAR

Abstract

Bakteriyofajlar, çoklu ilaç dirençli patojen bakterilerin yaygın olması nedeniyle gıda endüstrisinde ve faj terapisinde alternatif antibakteriyel ajanlar olarak kabul edilmektedirler. Bu çalışmanın amacı, gıda kaynaklı patojen Escherichia coli O157:H7'ye etkili litik bakteriyofajları izole etmektir. Bakteriyofajların taranması için kanalizasyon, balık çiftliklerinin, kesimhanelerin ve gıda fabrikalarının atık sularından toplam 51 örnek toplanmış ve 18 bakteriyofaj izole edilmiştir. Saflaştırılmış faj örneklerin titreleri 1.7×104-1.02×108 PFU/ml arasında değişmiştir. İzole edilen fajların tümü, E. coli O157: H7 suşlarına karşı litik aktiviteye sahip olduğu ve bu nedenle berrak plaklar oluşturduğu gözlenmiştir. On sekiz fajdan dördünün, E. coli O157:H7'ye ek olarak CFAI, ATCC 25922 ve DSa dâhil olmak üzere diğer E. coli suşlarına karşı enfektif oldukları bulunmuştur. Sadece iki fajın Salmonella Kentucky DMC35'e karşı enfektif olduğu gözlenmiştir. Eco-OH-fajları, E. coli O157:H7 suşlarına karşı 0.5 ila 0.1 arasında EOP değerleri ile yüksek enfeksiyon kabiliyetine sahip olduğu bulunmuştur.

Keywords

• Bakteriyofaj
• Escherichia coli O157:H7
• izolasyon
• konak hücre aralığı
• plak etkinliği

LYTIC BACTERIOPHAGES EFFECTIVE AGAINST ESCHERICHIA COLI O157:H7, A FOODBORNE PATHOGEN

Abstract

Bacteriophages are considered as alternative antibacterial agents in the food industry and phage therapy due to the widespread of multidrug-resistant pathogen bacteria. The objective of this study was to isolate lytic bacteriophages target to foodborne pathogen Escherichia coli O157:H7. For screening of bacteriophages, 51 samples were collected from sewage, wastewaters of fish farms, slaughterhouses and food factories, and 18 bacteriophages were isolated. The titer of the purified phages samples were changed among 1.7×104-1.02×108 PFU/ml. All of the isolated phages had lytic activity against E. coli O157:H7 strains and thus formed clear plaques. Four of 18 phages were found to have inhibitory effects against other E. coli strains including CFAI, ATCC 25922 and DSα in addition to E. coli O157:H7. It was observed that only two phages were infective against Salmonella Kentucky DMC35. The Eco-OH-phages were highly infection ability with EOP values from 0.5 to 0.1 against E. coli O157:H7 strains. 

Keywords

• Bacteriophage
• Escherichia coli O157:H7
• isolation
• host range
• efficiency of plating

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