AKSARAY İLİNDE SATIŞA SUNULAN TULUM PEYNİRLERİNDEN İZOLE EDİLEN ENTEROKOKLARIN ANTİBİYOTİK DİRENÇLİLİKLERİ İLE HEMOLİTİK AKTİVİTELERİNİN BELİRLENMESİ

Abstract

Bu çalışmada, Aksaray ilinde satışa sunulan tulum peyniri örneklerinden izole edilen enterokokların hemolitik aktivitesi ile antibiyotik duyarlılığının değerlendirilmesi amaçlanmıştır. İzolatlar, 16S rRNA gen sekanslaması sonucunda E. faecium (n = 30), E. faecalis (n = 25) ve E. durans (n = 7) olarak tanımlanmıştır. Üç izolat (E. faecalis ATC12, E. faecium ATC49 ve E. faecium ATC54) ß-hemolitik aktivite gösterirken, diğerlerinin hemolitik aktivite göstermediği saptanmıştır. İzolatların toplamda nalidiksik aside (%100), oksasiline (%92) ve steptomisine (%72.6) karşı orta veya yüksek derecede direnç gösterdiği saptanmıştır. E. faecalis suşlarının klinik olarak önem arz eden çeşitli antibiyotiklere E. faecium ve E. durans suşlarından daha dirençli fenotipleri olduğu görülmüştür. Çoklu antibiyotik direncinin %41.93 olduğu saptanmıştır. Sonuçlar değerlendirildiğinde Aksaray'da üretilen Tulum peynirinin gıda yolu ile antibiyotik direncinin yayılmasında tehlikeli bir araç olduğu kanısına varılmıştır.

Keywords

• Tulum peyniri
• Enterococcus
• hemolitik aktivite
• antibiyotik dirençlik

ANTIBIOTIC RESISTANCE AND HEMOLYTIC ACTIVITY IN ENTEROCOCCI ISOLATED FROM TULUM CHEESE SOLD IN AKSARAY PROVINCE

Abstract

This study aimed to evaluate the hemolytic activity and antibiotic susceptibility of enterococci isolated from Tulum cheese samples sold in Aksaray province. The isolates were identified as E. faecium (n = 30), E. faecalis (n = 25), and E. durans (n = 7) as a result of 16S rRNA gene sequencing. Three strains (E. faecalis ATC12, E. faecium ATC49, and E. faecium ATC54) exhibited ß-hemolytic activity, whereas others were non-hemolytic. Enterococci were found to have an intermediary or high resistance to nalidixic acid (%100), oxacillin (92%), and streptomycin (72.6%), respectively. E. faecalis strains had more resistant phenotypes to various clinically significant antibiotics than E. faecium and E. durans. Multi-drug resistance was found in 41.93% of the isolates. According to the results, Tulum cheese produced in Aksaray could be a potential vehicle for the transmission of antibiotic resistance via the food chain.

Keywords

• Enterococcus
• hemolytic activity
• virulence factor
• antibiotic resistance

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