SİYAH HAVUÇ POSASINDAN ANTOSİYANİNLERİN EKSTRAKSİYONUNA FARKLI ÇÖZGEN ve ASİT KONSANTRASYONLARININ ETKİLERİ

Year 2015, Volume: 40 Issue: 3, 149 - 156, 01.06.2015

Erdal Ağçam Asiye Akyıldız

Abstract

Bu çalışma ile ülkemizde yetiştiriciliği yapılan ve meyve-sebze suyu işleme sanayimizde yoğun işlenensiyah havucun artan posasından, antosiyaninlerin farklı asit konsantrasyonlarındaki çözgenlerle gerikazanım düzeyleri belirlenmiştir. Farklı ekstraktlardan elde edilen bulgulara göre siyah havuç posasınıntoplam monomerik antosiyanin miktarları 656.2-1191.9 mg/kg arasında değiştiği belirlenmiştir. Siyahhavuç posasından antosiyaninlerin ekstraksiyonunda en etkili çözücü asitlendirilmiş metanol (MeOH)iken; bunu asitlendirilmiş etanol (EtOH) ve saf su takip etmiştir. Çözgenlerin asitlik düzeyinin antosiyaningeri kazanımı üzerine önemli düzeyde etki ettiği tespit edilmiştir. HPLC sonuçlarına göre antosiyaninbileşenlerden siyanidin-3-ksilozil-glukozil-galaktozit-ferulik asidin (298.3-398.8 mg/kg) ve siyanidin-3ksilozil-galaktozidin (217.7-330.4 mg/kg) siyah havuç posasında en baskın oldukları belirlenmiştir

Keywords

Siyah havuç posası, ekstraksiyon, monomerik antosiyanin, antosiyanin bileşenleri

EFFECTS of DIFFERENT SOLVENTS and ACID CONSANTRATIONS on EXTRACTION of ANTHOCYANINS from BLACK CARROT POMACE

Abstract

In this study, effects of different solvents and acid concentrations on extraction of anthocyanins fromblack carrot pomace obtained after processing in fruit-vegetable juicing industry were investigated.According to results from different extraction processes, total monomeric contents of black carrot pomaceranged between 656.2-1191.9 mg/kg. The best solvent for anthocyanins extraction from black carrotpomace was determined as acidified methanol (MeOH); it was followed by acidified ethanol (EtOH)and pure water. Acidity levels of the solvents significantly affected recovery of anthocyanins from blackcarrot pomace. Based on HPLC results, the most abundant anthocyanins were cyanidin-3-xylosidegalactoside-glucoside-ferulic acid (298.3-398.8 mg/kg) and cyanidin-3-xyloside-galactoside(217.7-330.4 mg/kg) in the black carrot pomace

Keywords

Black carrot pomace, extraction, monomeric anthocyanin compounds

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