Pseudomonas putida İLE İZOÖJENOLDEN DOĞAL VANİLİN ÜRETİMİNDE BAZI ORTAM KOŞULLARININ MOLAR VERİM ÜZERİNE ETKİSİ

Year 2020, Volume: 45 Issue: 1, 9 - 19, 01.12.2019

Murat Yılmaztekin , Hüseyin Karakaya

https://doi.org/10.15237/gida.GD19112

Cited By: 1

Abstract

Vanilin, dünyada en çok kullanılan aroma maddelerinden
biridir. Son yıllarda, doğal vanilin üretimindeki yetersiz arz ve yüksek
maliyet nedeniyle araştırmalar biyodönüşüm yolları üzerine yoğunlaşmıştır. Bu
çalışmada, Pseudomonas putida (HUT 8100) kültürü kullanılarak
izoöjenolden doğal vanilin üretim imkânı araştırılmış; sıcaklık, pH ve
havalandırma koşullarının vanilin üretimi üzerine etkileri incelenmiştir. Elde
edilen sonuçlar, kullanılan P. putida kültürünün tek karbon kaynağı
olarak izoöjenolü kullanabildiği ve bunu vaniline dönüştürme yeteneğine sahip
olduğunu göstermiştir. En yüksek vanilin üretim kapasitesine 28°C’de,
biyodönüşüm ortamının başlangıç pH’sı 6.3 olarak ayarlandığında ulaşılmıştır.
Havalandırmanın kesilmesiyle biyokatalistin üretkenliğinde belirgin bir düşüş
gözlenmiştir. Üretim için en uygun şartların seçilmesiyle 120 saat sonunda
%25.1 molar verimle 877.9 mg/L düzeyinde bir üretim gerçekleştirilmiştir.
Yapılacak daha ileri optimizasyon çalışmaları ile birlikte üretilen vanilin
miktarının arttırılabileceği ve yöntemin endüstriyel proseslere uygun hale
getirilebileceği düşünülmektedir. 

Keywords

Vanilin, izoöjenol, biyodönüşüm, doğal aroma, verim

Supporting Institution

İnönü Üniversitesi

Project Number

2015-17

Thanks

Bu çalışma İnönü Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından 2015-17 proje numarasıyla desteklenmiştir. Yazarlar, mikroorganizma kültürünün temininde verdikleri destekten ötürü HUT Culture Collection’a teşekkür etmektedir.

EFFECT OF CULTURE CONDITIONS ON MOLAR YIELD OF NATURAL VANILLIN PRODUCTION FROM ISOEUGENOL BY Pseudomonas putida

Abstract

Vanillin is one of the most widely used aroma compounds
in the world. In recent years, studies are focused on bioconversion routes due
to insufficient supply and high cost of natural vanillin production. In this
study, possibility of natural vanillin production by using Pseudomonas
putida and effects of temperature, pH and aeration conditions on the
production yield were investigated. Results showed that P. putida
culture had ability to use isoeugenol as sole carbon source and convert it into
vanillin. The highest production yield was reached at 28°C when setting the
initial pH of bioconversion medium at 6.3. A distinct fall was observed in
productivity of biocatalyst by interruption of aeration. Using optimal
conditions, 877.9 mg/L vanillin production was achieved with 25.1% molar yield
in 120 h. It is thought that produced vanillin may be enhanced with further
optimization studies and the method can be made suitable for industrial
processes.

Keywords

Vanillin, isoeugenol, bioconversion, natural aroma, Pseudomonas putida

Project Number

2015-17

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