Optimization of Microwave-assisted Extraction of Phenolics from Organic Strawberry Using Response Surface Methodology
Year 2017,
, 143 - 154, 19.06.2017
Aysel Elik
,
Derya Koçak Yanık
Fahrettin Göğüş
Abstract
The effects of
microwave- assisted extraction (MAE) were investigated on extraction of
phenolic compounds from strawberry fruit. Response surface methodology (RSM)
was used to optimize the extraction conditions. A face-centered central
composite design (FCCCD) was employed to determine the effects of independent
variables such as microwave power (100-300 W), extraction time (2-16 min) and
solvent to sample ratio (5:1-25:1 mL g-1) on the extraction yield
and total phenolic content (TPC). Optimized conditions were determined as 265 W
of microwave power, 2 min of extraction time and 24:1 mL g-1 of
solvent to sample ratio. The maximum predicted extraction yield and TPC under
the optimum conditions were 8.23 % and 19.65 mg GAE g-1
dry strawberry, respectively. Total
anthocyanin content (TAC), DPPH·EC50
and FRAP values of extracts produced at optimum conditions were determined as
2.3 mg Cyn-3-glu g-1 dry strawberry, 1.67 mg dry strawberry mL-1 and 197.83 μmoles TE g-1
dry strawberry, respectively.
References
- Ayala-Zavala, J. F., Wang, S. Y., Wang, C. Y., González-Aguilar, G. A., 2007. High oxygen treatment increases antioxidant capacity and postharvest life of strawberry fruit. Food Technology and Biotechnology, 45 (2): 166-173.
- Azmir, J., Zaidul, I. S. M., Rahman, M. M., Sharif, K. M., Mohamed, A., Sahena, F., Jahurul, M. H. A., Ghafoor, K., Norulaini, N. A. N., Omar, A. K. M., 2013. Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering, 117 (4): 426-436.
- Bai, X., Qiu, A., Guan, J., 2007. Optimization of microwave-assisted extraction of antihepatotoxic triterpenoid from Actinidia deliciosa root and its comparison with conventional extraction methods. Food Technology & Biotechnology, 45 (2): 174-180.
- Ballard, T. S., Mallikarjunan, P., Zhou, K. Q., O'Keefe, S., 2010. Microwave-assisted extraction of phenolic antioxidant compounds from peanut skins. Food Chemistry, 120 (4): 1185-1192.
- Benzie, I. F. F., Strain, J. J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay. Analytical Biochemistry, 239 (1): 70-76.
- Brandwilliams, W., Cuvelier, M. E., Berset, C., 1995. Use of a free-radical method to evaluate antioxidant activity. Food Science and Technology (LWT), 28 (1): 25-30.
- Carniel, N., Dallago, R. M., Dariva, C., Bender, J. P., Nunes, A. L., Zanella, O., Bilibio, D., Luiz Priamo, W., 2016. Microwave‐assisted extraction of phenolic acids and flavonoids from Physalis angulata. Journal of Food Process Engineering, 40(3): 1-11.
- Chan, C. H., Yusoff, R., Ngoh, G. C., Kung, F. W. L., 2011. Microwave-assisted extractions of active ingredients from plants. Journal of Chromatography A, 1218 (37): 6213-6225.
- Chen, L., Jin, H., Ding, L., Zhang, H., Li, J., Qu, C., Zhang, H., 2008. Dynamic microwave-assisted extraction of flavonoids from Herba epimedii. Separation and Purification Technology, 59 (1): 50-57.
- Del Rio, D., Borges, G., Crozier, A., 2010. Berry flavonoids and phenolics: bioavailability and evidence of protective effects. British Journaol of Nutrition, 104 Suppl 3: S67-90.
- Food and Agriculture Organization (FAOSTAT). Statistical Database; 2014. Available from http://faostat.fao.org.
- Fortalezas, S., Tavares, L., Pimpão, R., Tyagi, M., Pontes, V., Alves, P. M., McDougall, G., Stewart, D., Ferreira, R. B., Santos, C. N., 2010. Antioxidant properties and neuroprotective capacity of strawberry tree fruit (Arbutus unedo). Nutrients, 2 (2): 214-229.
- Gao, M., Song, B. Z., Liu, C. Z., 2006. Dynamic microwave-assisted extraction of flavonoids from Saussurea medusa Maxim cultured cells. Biochemical Engineering Journal, 32 (2): 79-83.
- Garcia-Salas, P., Morales-Soto, A., Segura-Carretero, A., Fernandez-Gutierrez, A., 2010. Phenolic compound extraction systems for fruit and vegetable samples. Molecules, 15 (12): 8813-8826.
- Garofulic, I. E., Dragovic-Uzelac, V., Jambrak, A. R., Jukic, M., 2013. The effect of microwave assisted extraction on the isolation of anthocyanins and phenolic acids from sour cherry Marasca (Prunus cerasus var. Marasca). Journal of Food Engineering, 117 (4): 437-442.
- Hornedo-Ortega, R., Krisa, S., Garcia-Parrilla, M. C., Richard, T., 2016. Effects of gluconic and alcoholic fermentation on anthocyanin composition and antioxidant activity of beverages made from strawberry. Food Science and Technology (LWT), 69: 382-389.
- Huang, W. Y., Cai, Y. Z., Zhang, Y., 2009. Natural phenolic compounds from medicinal herbs and dietary plants: Potential use for cancer prevention. Nutrition and cancer, 62 (1): 1-20.
- Kafkas, E., Koşar, M., Paydaş, S., Kafkas, S., Başer, K. H. C., 2007. Quality characterictics of strawberry genotypes at different maturation stages. Food Chemistry, 100 (3): 1229-1236.
- Kaufmann, B., Christen, P., 2002. Recent extraction techniques for natural products: Microwave-assisted extraction and pressurised solvent extraction. Phytochemical Analysis, 13 (2): 105-113.
- Lee, J., Durst, R. W., Wrolstad, R. E., 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. Journal of AOAC International, 88 (5): 1269-1278.
- Ljevar, A., Ćurko, N., Tomašević, M., Radošević, K., Srček, V. G., Ganić, K. K., 2016. Phenolic composition, antioxidant capacity and in vitro cytotoxicity assessment of fruit wines. Food Technology and Biotechnology, 54(2): 145-155.
- Mandal, V., Mohan, Y., Hemalatha, S., 2007. Microwave assisted extraction—an innovative and promising extraction tool for medicinal plant research. Pharmacognosy Reviews, 1 (1): 7-18.
- Mendes, M., Carvalho, A. P., Magalhaes, J. M. C. S., Moreira, M., Guido, L., Gomes, A. M., Delerue-Matos, C., 2016. Response surface evaluation of microwave-assisted extraction conditions for Lycium barbarum bioactive compounds. Innovative Food Science & Emerging Technologies, 33: 319-326.
- Nile, S. H., Park, S. W., 2014. Edible berries: Bioactive components and their effect on human health. Nutrition, 30 (2): 134-144.
- Nohynek, L. J., Alakomi, H.-L., Kähkönen, M. P., Heinonen, M., Helander, I. M., Oksman-Caldentey, K.-M., Puupponen-Pimiä, R. H., 2006. Berry phenolics: antimicrobial properties and mechanisms of action against severe human pathogens. Nutrition and cancer, 54 (1): 18-32.
- Paredes-Lopez, O., Cervantes-Ceja, M. L., Vigna-Perez, M., Hernandez-Perez, T., 2010. Berries: improving human health and healthy aging, and promoting quality life--a review. Plant Foods Human Nutrition, 65 (3): 299-308.
- Pinelo, M., Rubilar, M., Jerez, M., Sineiro, J., Nunez, M. J., 2005. Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. Journal of Agricultural and Food Chemistry, 53 (6): 2111-2117.
- Sargent, D. J., Rys, A., Nier, S., Simpson, D. W., Tobutt, K. R., 2007. The development and mapping of functional markers in Fragaria and their transferability and potential for mapping in other genera. Theoretical and Applied Genetics, 114 (2): 373-384.
- Singleton, V. L., Orthofer, R., Lamuela-Raventos, R. M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152-178.
- Sinha, K., Das Saha, P., Datta, S., 2012. Response surface optimization and artificial neural network modeling of microwave assisted natural dye extraction from pomegranate rind. Industrial Crops and Products, 37 (1): 408-414.
- Wang, S. J., Chen, F., Wu, J. H., Wang, Z. F., Liao, X. J., Hu, X. S., 2007. Optimization of pectin extraction assisted by microwave from apple pomace using response surface methodology. Journal of Food Engineering, 78 (2): 693-700.
- Wu, T., Yan, J., Liu, R. H., Marcone, M. F., Aisa, H. A., Tsao, R., 2012. Optimization of microwave-assisted extraction of phenolics from potato and its downstream waste using orthogonal array design. Food Chemistry, 133 (4): 1292-1298.
- Yang, J., Liu, R. H., 2009. Induction of phase II enzyme, quinone reductase, in murine hepatoma cells in vitro by grape extracts and selected phytochemicals. Food Chemistry, 114 (3): 898-904.
- Zhang, H., Tsao, R., 2016. Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects. Current Opinion in Food Science, 8: 33-42.
- Zheng, X., Xu, X., Liu, C., Sun, Y., Lin, Z., Liu, H., 2013. Extraction characteristics and optimal parameters of anthocyanin from blueberry powder under microwave-assisted extraction conditions. Separation and Purification Technology, 104: 17-25.
Optimization of Microwave-assisted Extraction of Phenolics from Organic Strawberry Using Response Surface Methodology
Year 2017,
, 143 - 154, 19.06.2017
Aysel Elik
,
Derya Koçak Yanık
Fahrettin Göğüş
Abstract
The effects of
microwave- assisted extraction (MAE) were investigated on extraction of
phenolic compounds from strawberry fruit. Response surface methodology (RSM)
was used to optimize the extraction conditions. A face-centered central
composite design (FCCCD) was employed to determine the effects of independent
variables such as microwave power (100-300 W), extraction time (2-16 min) and
solvent to sample ratio (5:1-25:1 mL g-1) on the extraction yield
and total phenolic content (TPC). Optimized conditions were determined as 265 W
of microwave power, 2 min of extraction time and 24:1 mL g-1 of
solvent to sample ratio. The maximum predicted extraction yield and TPC under
the optimum conditions were 8.23 % and 19.65 mg GAE g-1
dry strawberry, respectively. Total
anthocyanin content (TAC), DPPH·EC50
and FRAP values of extracts produced at optimum conditions were determined as
2.3 mg Cyn-3-glu g-1 dry strawberry, 1.67 mg dry strawberry mL-1 and 197.83 μmoles TE g-1
dry strawberry, respectively.
References
- Ayala-Zavala, J. F., Wang, S. Y., Wang, C. Y., González-Aguilar, G. A., 2007. High oxygen treatment increases antioxidant capacity and postharvest life of strawberry fruit. Food Technology and Biotechnology, 45 (2): 166-173.
- Azmir, J., Zaidul, I. S. M., Rahman, M. M., Sharif, K. M., Mohamed, A., Sahena, F., Jahurul, M. H. A., Ghafoor, K., Norulaini, N. A. N., Omar, A. K. M., 2013. Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering, 117 (4): 426-436.
- Bai, X., Qiu, A., Guan, J., 2007. Optimization of microwave-assisted extraction of antihepatotoxic triterpenoid from Actinidia deliciosa root and its comparison with conventional extraction methods. Food Technology & Biotechnology, 45 (2): 174-180.
- Ballard, T. S., Mallikarjunan, P., Zhou, K. Q., O'Keefe, S., 2010. Microwave-assisted extraction of phenolic antioxidant compounds from peanut skins. Food Chemistry, 120 (4): 1185-1192.
- Benzie, I. F. F., Strain, J. J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay. Analytical Biochemistry, 239 (1): 70-76.
- Brandwilliams, W., Cuvelier, M. E., Berset, C., 1995. Use of a free-radical method to evaluate antioxidant activity. Food Science and Technology (LWT), 28 (1): 25-30.
- Carniel, N., Dallago, R. M., Dariva, C., Bender, J. P., Nunes, A. L., Zanella, O., Bilibio, D., Luiz Priamo, W., 2016. Microwave‐assisted extraction of phenolic acids and flavonoids from Physalis angulata. Journal of Food Process Engineering, 40(3): 1-11.
- Chan, C. H., Yusoff, R., Ngoh, G. C., Kung, F. W. L., 2011. Microwave-assisted extractions of active ingredients from plants. Journal of Chromatography A, 1218 (37): 6213-6225.
- Chen, L., Jin, H., Ding, L., Zhang, H., Li, J., Qu, C., Zhang, H., 2008. Dynamic microwave-assisted extraction of flavonoids from Herba epimedii. Separation and Purification Technology, 59 (1): 50-57.
- Del Rio, D., Borges, G., Crozier, A., 2010. Berry flavonoids and phenolics: bioavailability and evidence of protective effects. British Journaol of Nutrition, 104 Suppl 3: S67-90.
- Food and Agriculture Organization (FAOSTAT). Statistical Database; 2014. Available from http://faostat.fao.org.
- Fortalezas, S., Tavares, L., Pimpão, R., Tyagi, M., Pontes, V., Alves, P. M., McDougall, G., Stewart, D., Ferreira, R. B., Santos, C. N., 2010. Antioxidant properties and neuroprotective capacity of strawberry tree fruit (Arbutus unedo). Nutrients, 2 (2): 214-229.
- Gao, M., Song, B. Z., Liu, C. Z., 2006. Dynamic microwave-assisted extraction of flavonoids from Saussurea medusa Maxim cultured cells. Biochemical Engineering Journal, 32 (2): 79-83.
- Garcia-Salas, P., Morales-Soto, A., Segura-Carretero, A., Fernandez-Gutierrez, A., 2010. Phenolic compound extraction systems for fruit and vegetable samples. Molecules, 15 (12): 8813-8826.
- Garofulic, I. E., Dragovic-Uzelac, V., Jambrak, A. R., Jukic, M., 2013. The effect of microwave assisted extraction on the isolation of anthocyanins and phenolic acids from sour cherry Marasca (Prunus cerasus var. Marasca). Journal of Food Engineering, 117 (4): 437-442.
- Hornedo-Ortega, R., Krisa, S., Garcia-Parrilla, M. C., Richard, T., 2016. Effects of gluconic and alcoholic fermentation on anthocyanin composition and antioxidant activity of beverages made from strawberry. Food Science and Technology (LWT), 69: 382-389.
- Huang, W. Y., Cai, Y. Z., Zhang, Y., 2009. Natural phenolic compounds from medicinal herbs and dietary plants: Potential use for cancer prevention. Nutrition and cancer, 62 (1): 1-20.
- Kafkas, E., Koşar, M., Paydaş, S., Kafkas, S., Başer, K. H. C., 2007. Quality characterictics of strawberry genotypes at different maturation stages. Food Chemistry, 100 (3): 1229-1236.
- Kaufmann, B., Christen, P., 2002. Recent extraction techniques for natural products: Microwave-assisted extraction and pressurised solvent extraction. Phytochemical Analysis, 13 (2): 105-113.
- Lee, J., Durst, R. W., Wrolstad, R. E., 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. Journal of AOAC International, 88 (5): 1269-1278.
- Ljevar, A., Ćurko, N., Tomašević, M., Radošević, K., Srček, V. G., Ganić, K. K., 2016. Phenolic composition, antioxidant capacity and in vitro cytotoxicity assessment of fruit wines. Food Technology and Biotechnology, 54(2): 145-155.
- Mandal, V., Mohan, Y., Hemalatha, S., 2007. Microwave assisted extraction—an innovative and promising extraction tool for medicinal plant research. Pharmacognosy Reviews, 1 (1): 7-18.
- Mendes, M., Carvalho, A. P., Magalhaes, J. M. C. S., Moreira, M., Guido, L., Gomes, A. M., Delerue-Matos, C., 2016. Response surface evaluation of microwave-assisted extraction conditions for Lycium barbarum bioactive compounds. Innovative Food Science & Emerging Technologies, 33: 319-326.
- Nile, S. H., Park, S. W., 2014. Edible berries: Bioactive components and their effect on human health. Nutrition, 30 (2): 134-144.
- Nohynek, L. J., Alakomi, H.-L., Kähkönen, M. P., Heinonen, M., Helander, I. M., Oksman-Caldentey, K.-M., Puupponen-Pimiä, R. H., 2006. Berry phenolics: antimicrobial properties and mechanisms of action against severe human pathogens. Nutrition and cancer, 54 (1): 18-32.
- Paredes-Lopez, O., Cervantes-Ceja, M. L., Vigna-Perez, M., Hernandez-Perez, T., 2010. Berries: improving human health and healthy aging, and promoting quality life--a review. Plant Foods Human Nutrition, 65 (3): 299-308.
- Pinelo, M., Rubilar, M., Jerez, M., Sineiro, J., Nunez, M. J., 2005. Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. Journal of Agricultural and Food Chemistry, 53 (6): 2111-2117.
- Sargent, D. J., Rys, A., Nier, S., Simpson, D. W., Tobutt, K. R., 2007. The development and mapping of functional markers in Fragaria and their transferability and potential for mapping in other genera. Theoretical and Applied Genetics, 114 (2): 373-384.
- Singleton, V. L., Orthofer, R., Lamuela-Raventos, R. M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152-178.
- Sinha, K., Das Saha, P., Datta, S., 2012. Response surface optimization and artificial neural network modeling of microwave assisted natural dye extraction from pomegranate rind. Industrial Crops and Products, 37 (1): 408-414.
- Wang, S. J., Chen, F., Wu, J. H., Wang, Z. F., Liao, X. J., Hu, X. S., 2007. Optimization of pectin extraction assisted by microwave from apple pomace using response surface methodology. Journal of Food Engineering, 78 (2): 693-700.
- Wu, T., Yan, J., Liu, R. H., Marcone, M. F., Aisa, H. A., Tsao, R., 2012. Optimization of microwave-assisted extraction of phenolics from potato and its downstream waste using orthogonal array design. Food Chemistry, 133 (4): 1292-1298.
- Yang, J., Liu, R. H., 2009. Induction of phase II enzyme, quinone reductase, in murine hepatoma cells in vitro by grape extracts and selected phytochemicals. Food Chemistry, 114 (3): 898-904.
- Zhang, H., Tsao, R., 2016. Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects. Current Opinion in Food Science, 8: 33-42.
- Zheng, X., Xu, X., Liu, C., Sun, Y., Lin, Z., Liu, H., 2013. Extraction characteristics and optimal parameters of anthocyanin from blueberry powder under microwave-assisted extraction conditions. Separation and Purification Technology, 104: 17-25.