Unlocking the Antioxidant Potential of Calendula Officinalis: A Comparative Study of Extraction Methods

Abstract

Calendula officinalis which is also known as marigold is a species of plant in the daisy family that has antioxidant, antiinflammatory, cytotoxic, and antitumor properties. In this study we tried to analyze the antioxidant activity of calendula officinalis flowers extract. Three different extraction methods that are soxhlet, maceration, and ultrasonic procedures are applied to the calendula officinalis flowers and antioxidant activities for each extract is analyzed and compared. Methods: In this study, soxhlet, maceration and ultrasonic extraction methods and ethanol solvent are used to obtain calendula officinalis extracts. The antioxidant activity of the extracts with different concentration are investigated under DPPH solution. Results: DPPH radical scavenging activity for the extract obtained using soxhlet procedure is higher than the other two procedures that are maceration and ultrasonic. With increasing calendula officinalis extract concentration, it is observed that the antioxidant activity is also increased as expected. Conclusions: In conclusion, calendula officinalis flowers perform very high antioxidant property and the soxhlet method can be considered as the best method for the extraction. Further researches for the application of the extract can be studied.

Keywords

• calendula officinalis
• extraction
• antioxidant
• radical scavenging
• antioxidant activity

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