Determination of Biochemical Profile of Bilecik Propolis

Abstract

Önemli bir arı ürünü olan propolis antioksidan, antimikrobiyal, antitumoral, antiinflamatuar özellikleri nedeniyle apiterapi uygulamalarında ve günlük yaşamda gıda takviyesi olarak kullanılmaktadır. Propolisin özellikleri elde edildiği bölgeye, toplanma biçimine ve toplanma zamanına bağlı olarak değiştiğinden her kovandan veya bölgeden aynı özelliklere sahip propolis eldesi mümkün olmamaktadır. Yapılan bu çalışmayla Marmara Bölgesinde yer alan Bilecik ilinden temin edilen ham propolis örneklerinin biyokimyasal özellikleri belirlendi. Meşe, kayın, köknar, ıhlamur, kestane ağaçları ile çevrili olan Bilecik orman bakımından oldukça zengindir. Bu nedenle de zengin bir floraya sahiptir. Bu amaçla; %70’lik etil alkol çözeltisi kullanılarak ham propolislerden etanolik propolis özütleri hazırlandı. Hazırlanan propolis ekstraktlarının balsam miktarı, vaks miktarı, toplam polifenol miktarı ve toplam flavonoid miktarı belirlendi. GC/MS kullanılarak ham propolislerin içerdiği bileşenler tespit edildi. Propolis için önemli bir değer olan balsam miktarının % 13 ile %52 arasında değiştiği; toplam polifenol miktarının ise 11 mg GAE/mL ile 76 mg GAE/mL arasında değiştiği belirlendi. Propolis örneklerinin aldehitler, alifatik asit ve esterler, alkoller, hidrokarbonlar, karboksilik asit esterleri, ketonlar, terpenler, yağ asitleri ve diğer bileşenler bakımından zengin olduğu belirlendi. 

Keywords

• : Propolis,phenolic components,characterization,balsam

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