Carrier Free Cross-linked Peroxidase Aggregates: Synthesis and Characterization
Abstract
In this study, cross-linked Horseradish Peroxidase HRP aggregates were prepared. Bovin Serum Albumin BSA ,a proteic feeder, and glutaraldehyde, a bifunctional reactive, a crosslinker agent has also been used. The initialenzyme concentration 0.05 mg/ml , BSA 5 mg and glutaraldehyde GA 4 µl/ml amounts were optimized andmaximum immobilized activities were obtained. The kinetic parameters of free and immobilised enzyme have beencalculated by using Lineweaver-Burk plots. The saturation constant Km and the maximum reaction rate Vm offree enzyme were found as 2.33 mM and 0.670mmol/l.sec, respectively. The apparent kinetic parameters Kmapp andVmapp of cross linked enzyme aggregates have been calculated as 33.4 mM and 0.127 mmol/l.sec, respectively
Keywords
References
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Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
August 1, 2008
Submission Date
-
Acceptance Date
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Published in Issue
Year 2008 Volume: 36 Number: 3
APA
Topçular, C., & Ayhan, H. (2008). Carrier Free Cross-linked Peroxidase Aggregates: Synthesis and Characterization. Hacettepe Journal of Biology and Chemistry, 36(3), 255-261. https://izlik.org/JA87MZ86FC
AMA
1.Topçular C, Ayhan H. Carrier Free Cross-linked Peroxidase Aggregates: Synthesis and Characterization. HJBC. 2008;36(3):255-261. https://izlik.org/JA87MZ86FC
Chicago
Topçular, Ceren, and Hakan Ayhan. 2008. “Carrier Free Cross-Linked Peroxidase Aggregates: Synthesis and Characterization”. Hacettepe Journal of Biology and Chemistry 36 (3): 255-61. https://izlik.org/JA87MZ86FC.
EndNote
Topçular C, Ayhan H (August 1, 2008) Carrier Free Cross-linked Peroxidase Aggregates: Synthesis and Characterization. Hacettepe Journal of Biology and Chemistry 36 3 255–261.
IEEE
[1]C. Topçular and H. Ayhan, “Carrier Free Cross-linked Peroxidase Aggregates: Synthesis and Characterization”, HJBC, vol. 36, no. 3, pp. 255–261, Aug. 2008, [Online]. Available: https://izlik.org/JA87MZ86FC
ISNAD
Topçular, Ceren - Ayhan, Hakan. “Carrier Free Cross-Linked Peroxidase Aggregates: Synthesis and Characterization”. Hacettepe Journal of Biology and Chemistry 36/3 (August 1, 2008): 255-261. https://izlik.org/JA87MZ86FC.
JAMA
1.Topçular C, Ayhan H. Carrier Free Cross-linked Peroxidase Aggregates: Synthesis and Characterization. HJBC. 2008;36:255–261.
MLA
Topçular, Ceren, and Hakan Ayhan. “Carrier Free Cross-Linked Peroxidase Aggregates: Synthesis and Characterization”. Hacettepe Journal of Biology and Chemistry, vol. 36, no. 3, Aug. 2008, pp. 255-61, https://izlik.org/JA87MZ86FC.
Vancouver
1.Ceren Topçular, Hakan Ayhan. Carrier Free Cross-linked Peroxidase Aggregates: Synthesis and Characterization. HJBC [Internet]. 2008 Aug. 1;36(3):255-61. Available from: https://izlik.org/JA87MZ86FC