Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels

Nazlı Sökmen; Ferah Bican; Fatma Ayhan; Hakan Ayhan

Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels

Abstract

In this study, controlled drug release devices of poly ethylene glycol-diacrylate PEG-DA hydrogels were preparedby free radical UV polymerization. PEG-DA macromere was cross-linked with different percentages of ethylene glycoldimethacrylate EGDMA . The chelating agent, ethylene diamine tetraacetic acid EDTA , was added at three differentconcentrations. Gentamicin sulphate was incorporated into the hydrogel during photopolymerization and its releasekinetics was tested spectrophotometrically at 255 nm in phosphate buffer pH 7.4 . Results indicate that the presenceof chelating agent leads to a prolonged drug release. Gentamicin release from hydrogel synthesized by using lowchelating and cross-linking agent concentrations indicate slow release time. The effect of chelating agentconcentration eliminated when the amount of cross-linking agent increased while drug release time is still higher thancontrol group which was prepared in the absence of EDTA. It is interesting to note that the gentamicin release timeprolonged when chelating agent decreased at constant cross-linking agent concentration

Keywords

Chelating agent,PEG-DA,ethylene diamine tetraacetic acid EDTA,Hydrogel

References

1. Peppas N.A., Ward, J.H. Preparation of controlled release systems by free – radical UV polymerizations in the presence of a drug, Journal of Controlled Release, 711: 183-192, 2001.
2. Kanjickal, D.G., Lopina, S.T., Modeling of drug release from polymeric delivery systems—a review, Crit. Rev. Ther. Drug Carr. Syst. 21: 345–386, 2004.
3. Scott, R.A., Peppas, N.A., Highly crosslinked, PEG-containing copolymers for sustained solute delivery, Biomaterials, 20: 1371-1380, 1999.
4. Hoffman, A.S. Hydrogels for biomedical applications. Advanced Drug Delivery Reviews, 43: 3-12, 2002.
5. Nguyen, K.T., West, J.L., Photopolymerizable hydrogels for tissue engineering applications, Biomaterials, 23: 4307–4314, 2002.
6. Kost, J., Langer, R., Responsive polymeric delivery systems, Advanced Drug Delivery Reviews, 46: 125–148, 2001.
7. Francis, S., Varshney, L., Studies on radiation synthesis of PVA/EDTA hydrogels, Radiation Physics and Chemistry, 74: 310 – 316, 2001.
8. Otto J. Grundler, Arnold T.M van der Steen, Joel Wilmot., Overview of the European Risk Assessment on EDTA, ACS Symposium Series 910, 336-347, 2005.

Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Nazlı Sökmen This is me

Ferah Bican This is me

Fatma Ayhan This is me

Hakan Ayhan This is me

Publication Date

December 1, 2008

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2008 Volume: 36 Number: 4

IZ

https://izlik.org/JA68YR97DF

Cite

RIS / Bibtex

APA

Sökmen, N., Bican, F., Ayhan, F., & Ayhan, H. (2008). Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels. Hacettepe Journal of Biology and Chemistry, 36(4), 347-352. https://izlik.org/JA68YR97DF

AMA

1.Sökmen N, Bican F, Ayhan F, Ayhan H. Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels. HJBC. 2008;36(4):347-352. https://izlik.org/JA68YR97DF

Chicago

Sökmen, Nazlı, Ferah Bican, Fatma Ayhan, and Hakan Ayhan. 2008. “Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels”. Hacettepe Journal of Biology and Chemistry 36 (4): 347-52. https://izlik.org/JA68YR97DF.

EndNote

Sökmen N, Bican F, Ayhan F, Ayhan H (December 1, 2008) Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels. Hacettepe Journal of Biology and Chemistry 36 4 347–352.

IEEE

[1]N. Sökmen, F. Bican, F. Ayhan, and H. Ayhan, “Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels”, HJBC, vol. 36, no. 4, pp. 347–352, Dec. 2008, [Online]. Available: https://izlik.org/JA68YR97DF

ISNAD

Sökmen, Nazlı - Bican, Ferah - Ayhan, Fatma - Ayhan, Hakan. “Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels”. Hacettepe Journal of Biology and Chemistry 36/4 (December 1, 2008): 347-352. https://izlik.org/JA68YR97DF.

JAMA

1.Sökmen N, Bican F, Ayhan F, Ayhan H. Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels. HJBC. 2008;36:347–352.

MLA

Sökmen, Nazlı, et al. “Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels”. Hacettepe Journal of Biology and Chemistry, vol. 36, no. 4, Dec. 2008, pp. 347-52, https://izlik.org/JA68YR97DF.

Vancouver

1.Nazlı Sökmen, Ferah Bican, Fatma Ayhan, Hakan Ayhan. Chelating Agent Effect on the Release of Gentamicin from PEG-DA Hydrogels. HJBC [Internet]. 2008 Dec. 1;36(4):347-52. Available from: https://izlik.org/JA68YR97DF