Glucosidase Inhibitory and Radical Scavenging Properties of Lichen Metabolites Salazinic Acid, Sekikaic Acid and Usnic Acid

Abstract

This paper reports α- and β-glucosidase inhibitory and radical scavenging potential of the metabolites; salazinic acid, sekikaic acid and usnic acid isolated from three terrestrial natural lichen species Ramalina celastri, Ramalina nervulosa and Ramalina pacifica of the genus Ramalinaceae. Lichen metabolites showed both glucosidase inhibitory and radical scavenging capacity. Half-inhibiting concentration IC50 values of the lichen metabolites; salazinic acid, sekikaic acid and usnic acid are 13.8 to 18.1, 13.8 to 14.6 and 17.7 to 18.9 μg/ mL for the α- and β-glucosidase inhibition. Scavenging of radicals are found with an IC50 values of salazinic acid, sekikaic acid and usnic acid are 17.2 to 20.5, 13.7 to 17.4 and 18.8 to 25.5 μg/mL respectively. Inhibition kinetic studies of the metabolite towards α- and β-glucosidase showed sekikaic acid and salazinic acid have competitive inhibition towards α-glucosidase activity and noncompetitive inhibition for β-glucosidase activity. Usnic acid showed noncompetitive type of inhibition for α-glucosidase and uncompetitive for β-glucosidase activity.

Keywords

• Lichen metabolite,glucosidase inhibition,radical scavenging activity

Liken Metabolitleri Salazinik, Sekikaik ve Usnik Asitin Glikosidaz Engelleyici ve Radikal Süpürücü Özelliği

Abstract

Bu makale, Ramalinaceae ailesine ait Ramalina celastri, Ramalina nervulosa ve Ramalina pacifica isimli üç doğal kara likeninden izole edilen salazinik, sekikaik ve usnik asit metabolitlerinin α- ve β-glukosidaz engelleyici ve radikal süpürücü potansiyelini anlatmaktadır. Liken metabolitleri her iki glukosidaz engelleme ve radikal süpürücü kapasiteye sahiptir. α- ve β- glukosidaz inhibisyonu için liken metabolitlerinin yarı inhibisyon derişimi IC50 değerleri 13.8-18.1, 13.8-14.6 ve 17.7-18.9 µg/mL’dir. Salazinik, sekikaik ve usnik asitin IC50 radikal süpürme değerleri sırasıyla 17.2-20.5, 13.7-17.4 ve 18.8-25.5 µg/mL olarak bulunmuştur. Metabolitlerin α- ve β-glukosidaza yönelik inhibisyon kinetik çalışmaları sekikaik ve salazinik asitin α-glukosidaz aktivitesine yarışmalı ve β-glukosidaz aktivitesine yarışmasız inhibisyon yaptıklarını göstermektedir. Usnik asit α-glukosidaz aktivitesi için yarışmasız ve β-glukosidaz aktivitesi için yarışma dışı inhibisyon göstermiştir

Keywords

• Liken metabolit,glukosidaz inhibisyonu,radikal süpürme aktivitesi

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