Genetic Identification of Algas on The Basis of The Analysis of 18s rRNA and rbcl of Genes Nucleotide Sequence and Development of Technology of Aquaculture Cultivation

Abstract

Specific identification of green microalgas, perspective as feed additive for a fry stage of fishes was carried out. The culture was identified as Parachlorella kessleri. Technologies of aquaculture cultivation were developed: Parachlorella kessleri microalga, and also zooplankton: salt-water crustacean Artemia salina and fresh-water crustacean Daphnia magna in vitro for receiving feed additives for fishes.

Keywords

• Microalgas,PCR,primers,18S rRNA,rbcl genes,food fishes,forage production,artemia,water flea,technology of cultivation,incubation,biomass

18s rRNA ve rbcl Genlerinin Nükleotit Dizilimi Esasına Göre Alglerin Genetik Tanımlanması ve Su Ürünleri Yetiştiriciliği Teknolojisi Geliştirilmesi

Abstract

Y eşil mikroalglerin özgün bir şekilde tanımlanmasında, balıkların yavru aşamasında yem katkı maddesi olarak kulanımı gerçekleştirilmiştir. Kültür, Parachlorella kessleri olarak tanımlanmıştır. Parachlorella kessleri mikroalgi, ve ayrıca Zooplanktonun: tuzlu su kabuklu Artemia salina ve tatlı su kabuklu Daphnia magna in vitro balıklara yem katkı maddesi olarak kullanımı için su ürünleri yetiştiriciliği teknolojisi geliştirilmiştir

Keywords

• Mikroalgler,PCR primerler,18S rRNA,rbcL genleri,gıda balıklar,yem üretimi,artemya,su piresi,ekim teknolojisi,kuluçka,biyokütle

References

1. 1. The Concept of Development of Fishery of the Republic of Kazakhstan for 2007-2015 (The Resolution of the Government of the Republic of Kazakhstan of October 6, 2006 N 963).
2. 2. S.P. Vasser, N.V. Kondrat’yeva, N.P. Masyuk, A.O. Vodorosli Spravochnik, Kiev, Naukova dumka (1989) 605.
3. 3. I.V. Ivleva, Biological bases and methods of mass cultivation of fodder invertebrates, Moskva: Nauka, (1969) 171.
4. 4. G.T. Buzmakov, A.D. Polyakov, Zoology: biology and cultivation invertebrates / Successes of modern natural sciences, N2, (2010) 14-16.
5. 5. J.R. Manhart, Phylogenetic analysis of green plant rbcL sequences. Molecular Phylogenetics and Evolution, 3 (1994) 114-127.
6. 6. K. Kuznedelov, O. Timoshkin, V. Kumarev, Molecular phylogeny of planarians (Turbellaria, Tricladida Paludicola) of Lake Baikal established by comparative analysis of the nucleotide sequences of 18S ribosomal RNAs, Mol. Biol. (Moscow), 30 (1996) 1316–1325.
7. 7. L.A. Sirenko, A.I. Sakevich, L.F. Osipov, L.F. Lukina, M.I. Kuz’menko, V.N. Kozitskaya, I.M. Velichko, V.O. Myslovich, M.Y. Gavrilenko, V.V. Arendarchuk, Y.A. Kirpenko, Methods of fiziologo-biochemical research of algas in hydrobiological practice, Kiev: Naukova dumka, (1975) 247.
8. 8. A.M. Muzafarov, T.T. Taubayev, Cultivation and application of microalgas, Tashkent: Fan, (1984) 40-41. (Muzafarov A.M., Taubayev T.T.).

9. 9. V.I. Uspenskaya, Ecology and physiology of freshwater algae nutrition, Moscow: MGU, 1966. – 124 p. (Uspenskaya V.I.).
10. 10. E.G. Pringsheim, Bakterien und Cyanophyceen Ubereinstimmungen und Unterschiede, – Z.Osterr. bot., (1967) 114.
11. 11. V.D. Romanenko, Y.G. Krot, L.A. Sirenko, V.D. Solomatina, Biotechnology of cultivation of hydrobionts, Kiev, (1999) 264.