Year 2020, Volume 48 , Issue 2, Pages 147 - 157 2020-04-19

Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents

Yakup ATICI [1] , İbrahim BULDUK [2]


Artichoke leaf (Cynara Scolymus) extracts widely used in medicine. Chlorogenic Acids have antioxidant, antibacterial and antiviral properties is an compound.

In this study, after the artichoke (Cynara scolymus) leaves had  dried, powdered portions were extracted to with water. The results had expressed as ppm after  extracts  were analyzed by HPLC. It were determined that optimum conditions of extracting chlorogenic acid from Cynara Scolymus leaves with the ultrasonic assisted extraction with Response Surface Method (RSM) based on Box-Behnken design. The experimental conditions for this extraction efficiency were determined as extraction time (15-45 minutes), temperature (30-700C) and ultrasound power (25-75%).  The optimum conditions were determined as 47.65% ultrasound power, 35.18 minutes, 53.400 C temperature. The maximum yield of chlorogenic acid was determined to 10.05 ppm.

Also the total phenolic content was measured by UV -Vis spectrometry in order to see antioxidant activity.

Absorbance values of samples which are took from each sample  were measured at 765 nm in UV spectrometer. Optimum conditions were determined as 47.29% ultrasound power, 29.67 minutes, 55.33% ethanol concentration. Total phenolic content were determined as 70.24 ppm. According the to results ultrasonic-assisted extraction (UAE) showed can be an effective method for the extraction of chlorogenic acid active ingredient.

Chlorogenic Acid, Artichoke leaf (Cynara scolymus), Extraction, Optimization, RSM
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Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0002-6107-3081
Author: Yakup ATICI (Primary Author)
Country: Turkey


Orcid: 0000-0001-6172-7738
Author: İbrahim BULDUK
Institution: USAK UNIVERSITY
Country: Turkey


Dates

Publication Date : April 19, 2020

Bibtex @research article { hjbc511586, journal = {Hacettepe Journal of Biology and Chemistry}, issn = {2687-475X}, eissn = {2687-475X}, address = {Hacettepe Üniversitesi Fen Fakültesi, 06532, Beytepe/ ANKARA/ TÜRKİYE}, publisher = {Hacettepe University}, year = {2020}, volume = {48}, pages = {147 - 157}, doi = {10.15671/hjbc.511586}, title = {Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents}, key = {cite}, author = {Atıcı, Yakup and Bulduk, İbrahim} }
APA Atıcı, Y , Bulduk, İ . (2020). Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents . Hacettepe Journal of Biology and Chemistry , 48 (2) , 147-157 . DOI: 10.15671/hjbc.511586
MLA Atıcı, Y , Bulduk, İ . "Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents" . Hacettepe Journal of Biology and Chemistry 48 (2020 ): 147-157 <https://dergipark.org.tr/en/pub/hjbc/issue/53810/511586>
Chicago Atıcı, Y , Bulduk, İ . "Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents". Hacettepe Journal of Biology and Chemistry 48 (2020 ): 147-157
RIS TY - JOUR T1 - Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents AU - Yakup Atıcı , İbrahim Bulduk Y1 - 2020 PY - 2020 N1 - doi: 10.15671/hjbc.511586 DO - 10.15671/hjbc.511586 T2 - Hacettepe Journal of Biology and Chemistry JF - Journal JO - JOR SP - 147 EP - 157 VL - 48 IS - 2 SN - 2687-475X-2687-475X M3 - doi: 10.15671/hjbc.511586 UR - https://doi.org/10.15671/hjbc.511586 Y2 - 2020 ER -
EndNote %0 Hacettepe Journal of Biology and Chemistry Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents %A Yakup Atıcı , İbrahim Bulduk %T Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents %D 2020 %J Hacettepe Journal of Biology and Chemistry %P 2687-475X-2687-475X %V 48 %N 2 %R doi: 10.15671/hjbc.511586 %U 10.15671/hjbc.511586
ISNAD Atıcı, Yakup , Bulduk, İbrahim . "Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents". Hacettepe Journal of Biology and Chemistry 48 / 2 (April 2020): 147-157 . https://doi.org/10.15671/hjbc.511586
AMA Atıcı Y , Bulduk İ . Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents. HJBC. 2020; 48(2): 147-157.
Vancouver Atıcı Y , Bulduk İ . Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents. Hacettepe Journal of Biology and Chemistry. 2020; 48(2): 147-157.
IEEE Y. Atıcı and İ. Bulduk , "Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents", Hacettepe Journal of Biology and Chemistry, vol. 48, no. 2, pp. 147-157, Apr. 2020, doi:10.15671/hjbc.511586