Antioxidant and Antimicrobial Activities of Selected Turkish Honeys

Year 2008, Volume: 36 Issue: 2, 163 - 172, 01.04.2008

Sevgi Kolaylı Rezzan Aliyazıcıoğlu Esra Ulusoy Şengül Karaoğlu

Abstract

The purpose of this study was to investigate antioxidant and antimicrobial activities of selected honeys, chestnut n = 15 , Bayburt n = 8 and Anzer n = 7 from Turkey. Antioxidant activity was evaluated by the following three methods: scavenging of 2,2-diphenyl-1-picrylhydrazyl DPPH free radicals, total phenolics and ferric reducing/antioxidant power FRAP . Antimicrobial activity was studied by agar diffusion method by using eight bacteria and two fungi. The antioxidant activities were found to be related to the sample concentrations. The phenolic contents of the honeys were found to be significantly related with antioxidant activities with a correlation coefficient of R2 = 0.77. All samples showed moderate antimicrobial activity, especially against Helicobacter pylori, Staphylococcus aureus and Klepsiella pneumonia. No activity was observed against the two fungi, Candida albicans and Candida tropicalis.

Keywords

Anzer, Antioxidant, Antimicrobial, Honey

References

• 1. Lusby, P.E., Coombes, A. and Wilkinson, J.M., Honey: A potent agent for wound healing? J. Wound Care Ostomy Continence Nurs. 29, 295, 2002.
• 2. Schramm, D.D., Karim, M., Schrader, H.R., Holt, R.R., Cardetti M. and Keen, C.L., Honey with high levels of antioxidants can provide protection to healthy human subjects, J. Agric. Food Chem. 51, 1732, 2003.
• 3. Anklam, E., A review of the analytical methods to determine the geographical and botanical origin of honey, Food Chem. 63, 549, 1988.
• 4. Azeredo, L.C., Azeredo, M.A.A., De Souza, S.R. and Dutra, V.M.L., Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins, Food Chem. 80, 249, 2003.
• 5. Halliwel, B., Oxidative stress in cell culture: an under-appreciated problem. FEBS Letters, 540, 3, 2003.
• 6. Yaylacı, F., Kolaylı, S., Kuçük, M., Karaoğlu, S.A. and Ulusoy, E., Biological activities of trunk bark extract of five tree species from Anatolia, Turkey, Asian J. Chem. 19, 2241, 2007.
• 7. Halliwel, B., The antioxidant paradox, Lancet 355, 1179, 2000.
• 8. Halliwel, B., Dietary phenolics; Good, bad, or, indifferent for your health? Cardiovasc. Res. 73, 341,.2007.
• 9. Huang, D., Ou, B. and Prior, R.L., The Chemistry behind antioxidant capacity assays, J. Agric. Food Chem. 53, 1841, 2005.
• 10. Beratta, G., Granata, P., Ferrero, M., Orioli, M. and Maffei Facino, R., Standardization of antioxidant properties of honey by a combination of spectrophotometric / fluorimetric assays and chemometrics, Anal. Chim. Acta 533, 185, 2005.
• 11. Chen, L.A., Mehta, M., Berenbaum, A.R., Zanger, I. and Engeseth, N.J., Honeys from different floral sources as inhibitors of enzymatic browning in fruit and vegetable homogenates, J. Agric. Food Chem. 48, 4997, 2000.
• 12. Küçük, M., Kolaylı, S., Karaoğlu, S., Ulusoy, E., Baltacı, C. and Candan, F., Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chem. 100, 526, 2007.
• 13. Tüzen, M., Silici, S., Mendil, D. and Soylak, M., Trace element levels in honeys from different regions of Turkey, Food Chem. 103, 325, 2006.
• 14. Ozkırım, A. and Keskin, N., A survey of nosema apis of honey bees (Apis mellifera L.) producing the famous Anzer honey in Turkey, Z. Naturforsch C-A J. Biosci. 56, 918, 2001.
• 15. Mckibben, J. and Engeseth, N.J., Honey as a protective agent against lipid oxidation in ground Turkey, J. Agric. Food Chem. 50, 592, 2002.
• 16. Sorkun, K., Doğan, C. and Başoğlu, N., Physicochemical, Characteristics and Composition of Eucalypthus camaldulensis Dehnh. honey produced in Turkey, Apiacta 36, 182, 2001.
• 17. Tananaki, C.H., Thrasyvoulou, A., Giraudel, J.L. and Montury, M., Determination of volatile characteristics of Greek and Turkish pine honey samples and their classification by using Kohonen self organizing maps, Food Chem. 101, 1687, 2007.
• 18. Benzie, I.F.F. and Strain, J.J., The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: The FRAP assay, Anal. Biochem. 239, 70, 1996.
• 19. Yu, L., Haley, S., Perret, J., Harris, M., Wilson, J. and Qian, M., Free radical scavenging properties of wheat extracts, J. Agric. Food Chem. 50, 1619, 2002.
• 20. Slinkard, K. and Singleton, V.L., Total phenol analysis: automation and comparison with manual methods, Am. J. Enol. Viticult. 28, 49, 1977.
• 21. Perez, C., Pauli, M. and Bazerque, P., An antibiotic assay by the agar well diffusion method, Acta Biol. Med. Exp. 15, 113, 1990.
• 22. Kumazawa, S., Hamasaka, T. and Nakayama, T., Antioxidant activity of propolis of various geographic origins, Food Chem. 84, 329, 2004.
• 23. Ahn, M.R., Kumazawa, S., Usui, Y., Nakamura, J., Matsuka, M., Zhu, F. and Nakayama, T., Antioxidant activity and constituents of propolis collected in various areas of China, Food Chem. 101, 1383, 2007.
• 24. Rice-Evans, C.A., Miller, N.J. and Paganga, G., Antioxidant properties of phenolic compounds, Trends Plant Sci. 2, 152, 1997.
• 25. Higdon, J.V. and Frei, B., Tea catechine and polyphenols: Health effects, metabolism and antioxidant functions, Crit. Rev. Food Sci. Nutr. 43, 89, 2003.
• 26. Pulido, R., Bravo, L. and Saura-Calixto, F., Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay, J. Agric. Food Chem. 48, 3396, 2000.
• 27. Al-Mammary, M., Al-Meeri, A. and Al-Habori, M., Antioxidant activities and total phenolics of different types of honey, Nutr. Res. 22, 1041, 2002.
• 28. Robards, K., Prenzler, P.D., Tucker, G., Swatsitang, P. and Glover, W., Phenolic compounds and their role in oxidative processes in fruits, Food Chem. 66, 401,1999.
• 29. Moirin, P.L., Levy Junior, N.C., Custodio, A.R., Bretz, W.A. and Marcucci, M.C., Antibacterial activity of honey and propolis from Apis melliafera and Tetragonisca angustula against Staphylococus aureus, J. Appl. Microbiol. 95, 913, 2003.
• 30. Oradifiya, L.O., Adesina, S.K., Igbeneghu, O.A., Akinkunmi, E.O., Adetogun, G.E. and Salau, A.O., The effect to honey and surfactant type on the antibacterial properties of the leaf essential oil of Ocimum gratissimum Linn. Against common wound–infecting organisms, Inter. J. Aromatherapy 16, 57, 2006.
• 31. Cooper, R.A., Molan, P.C. and Harding, K.G., The sensitivity to honey of Gram-positive cocci of clinical significance isolated from wounds, J. App. Microbiol. 93, 857, 2002.
• 32. Weston, R.J., The contribution of catalase and other natural products to the antibacterial activity of honey: a review., Food Chem. 71, 235, 2000.
• 33. Ceylan, N. and Uğur, A., Investigation of in vitro antimicrobial activity of honey, Rev. Biol. 94, 363, 2001.