Carrier Free Cross-linked Peroxidase Aggregates: Synthesis and Characterization

Year 2008, Volume: 36 Issue: 3, 255 - 261, 01.08.2008

Ceren Topçular Hakan Ayhan

Abstract

In this study, cross-linked Horseradish Peroxidase HRP aggregates were prepared. Bovin Serum Albumin BSA ,a proteic feeder, and glutaraldehyde, a bifunctional reactive, a crosslinker agent has also been used. The initialenzyme concentration 0.05 mg/ml , BSA 5 mg and glutaraldehyde GA 4 µl/ml amounts were optimized andmaximum immobilized activities were obtained. The kinetic parameters of free and immobilised enzyme have beencalculated by using Lineweaver-Burk plots. The saturation constant Km and the maximum reaction rate Vm offree enzyme were found as 2.33 mM and 0.670mmol/l.sec, respectively. The apparent kinetic parameters Kmapp andVmapp of cross linked enzyme aggregates have been calculated as 33.4 mM and 0.127 mmol/l.sec, respectively

Keywords

cross-linked enzyme aggregates, horseradish peroxidase, bovine serum albumin, proteic feeder

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