Different Part of Mushroom Tissue Used as Modifier in Carbon Paste Sensor

Year 2013, Volume: 41 Issue: 3, 187 - 193, 01.09.2013

Nevila Broli Majlinda Vasjari

Abstract

An enzymatic carbon paste electrode for determination of phenolic compounds is developed. The electrode material was prepared by mixing the monodisperse graphite powder with paraffin and with crude mushroom tissue which contains polyphenol oxidase PPO in its living environment. The ratio between components carbon - tissue - paraffin , was investigated using SCV Stare Case Voltammetry techniques in 0.1 M sodium phosphate buffer, pH 7, scan rate 50 mV/s. The derivative voltammogrammes are used to evaluate the biosensor response. The analytical performance was closely related with the carbon powder granulometry. The optimal results regarding the background current and sensitivity 1.57 mA/ppm were obtained when carbon powder granulometry was 0.09-0.071 mm. It was also studied the activity of the PPO taken from four separated sections of mushroom body. Kinetic of enzymatic reaction resulted according to the Michaels–Menten mechanism. The biosensor response was tested toward different phenolic compounds. The best signal regarding the sensitivity of biosensor S=2.04 mA/ppm , correlation coefficient R2= 0.9997 and detection limit 0.7 ppm was obtained for hydroquinone and the other studied compounds are listed in these order: hydroquinone > catechol > phenol > m-cresol > 4-chlorophenol > p-cresol > 4-nitrophenol > 3-nitrophenol.

Keywords

Phenolic compound, carbon paste biosensor, PPO, crude tissue, Voltammetry

Karbon Pasta Biyosensöründe Dönüştürücü Olarak Kullanılan Mantar Dokusunun Farklı Bir Yönü

Abstract

F enolik bileşiklerin belirlenmesi için enzimatik bir karbon elektrot macunu geliştirilmiştir. Elektrot malzemesi parafin ve canlı ortamda polifenol oksidaz PPO içeren ham mantar doku ile birlikte tek dağılımlı monodisperse grafit tozunun karıştırılması ile hazırlanmıştır. Bileşenleri arasındaki oran karbon - doku parafin , 0.1 M sodyum fosfat tamponu, pH 7, ve 50 mV/s tarama hızında SCV Stare Case Voltammetry teknikleri kullanılarak araştırılmıştır. Türev voltamogramlar biyosensör yanıtını değerlendirmek için kullanılır. Analitik performans karbon tozu granülometrisi ile yakından ilgilidir. Karbon tozu granülometrisi 0,09-0,071 mm iken, arka akım ve hassasiyeti 1.57 mA / ppm ile ilgili olarak en iyi sonuçlar elde edildi. Ayrıca mantar gövdesinin ayrılmış dört bölümünden alınan PPO aktivitesi incelenmiştir. Enzimatik reaksiyonun kinetiği Michaels-Menten mekanizmasına göre sonuçlanmıştır. Biyosensör yanıtı, farklı fenolik bileşiklere karşı test edilmiştir. Hidrokinon için biyosensör duyarlılığı ile ilgili en iyi sinyal S = 2.04 mA / ppm , korelasyon katsayısı R2 = 0,9997 ve algılama sınırı 0.7 ppm elde edilmiştir ve diğer çalışılan bileşikler şu sırada verilmektedir: hidrokinon> katekol > fenol> m-kresol> 4-klorofenol> p-kresol> 4-nitrofenol> 3-nitrofenol

Keywords

Fenolik bileşik, karbon pasta biyosensör, PPO, ham doku, voltametri

References

• 1. C. Apetrei, I. Mirela, J.A. De Saja and M.L. RodriguezMendez, Carbon paste electrodes made from different carbonaceous materials: Application in the Study of Antioxidants, Sensors, 11 (2011) 1328.
• 2. G.K. Buckee, Determination of the volatile components of beer, J. Inst. Brewing, 98 (1992) 78,DOI: 10.1002/j.2050-0416.1992.tb01093.x.(1992).
• 3. E.A. Cummings, P. Mailley, S. Linquette-Maille, B.R. Eggins, E.T. McAdams, S. McFadden, Amperometric carbon paste biosensor based on plant tissue for the determination of total flavanol content in beers, The Analyst, 123 (1998) 1975.
• 4. F.O. Fatibello, K.O. Lupetti, O.D. Leite, I.C. Vieira, Determination of total phenols in wastewaters using a biosensor based on carbon paste modified with crude extract of jackfruit (Artocarpusintegrifolia L.). Electrochemical Sensor Analysis.Comprehensive Analytical Chemistry 49, Alegret and Merkoci (Eds) Elsevier B. (2007) 163.
• 5. F.O. Fatibello, K.O. Lupetti, O.D. Leite, I.C. Vieira, Electrochemical biosensors based on vegetable tissues and crude extracts for environmental, food and pharmaceutical analysis, Elect. Sens. Analy. Compr. Anal. Chem. 49, Alegret and Merkoci (Eds) Elsevier B., (2007) 357.
• 6. L. Fotouhi, M. Ganjavi and D. Nematollahi, Electrochemical Study of Iodide in the Presence of Phenol and o-Cresol: Application to the Catalytic Determination of Phenol and o-Cresol, Sensors, 4 (2004) 170.
• 7. Sh.A. Hanifah, L.Y. Heng and M. Ahmad, Effects of gold nanoparticles on the response of phenol biosensor containing photocurable membrane with tyrosinase, Sensors, 8 (2008) 6407.
• 8. W. Joseph, A.K. Stephen, L .Jie, R.S. Malcom and K. Rogers, Mushroom tissue- based biosensor for Inhibitor monitoring, Food Tech. Biotech., 34 (1996) 51.
• 9. S.H. Kazemia, K. Khajehb, Electrochemical studies of a novel biosensor based on the CuO nanoparticles coated with horseradish peroxidase to determine the concentration of phenolic compounds, J. Iran.Chem. Soc., 8 (2011) 152.
• 10. J. Markowskiand, W. Płocharski, Determination of phenolic compaund in apples and processed apple products, J. Fruit Ornament. Plant Res.,14 (2006) 133.
• 11. S. K. Ozoner, E. Erhan, F. Yilmaz, Enzyme Based Phenol Biosensors, Environmental biosensors, Chapter 15 edited by Vernon Somerset, ISBN 978- 953-307-486-3, 15 (2011) 320.
• 12. J. Ren, T.F Kang, R Xue, Ch-N Ge, Sh - Y Cheng, Biosensor based on a glassy carbon electrode modified with tyrosinase immobilized on multiwalled carbon nanotubes, Microchim. Acta, 174 (2011) 303.
• 13. M.C. Rodrıguez, G.A. Rivas, Glassy carbon paste electrodes modified with polyphenol oxidase analytical applications, Anal. Chim. Acta, 459 (2002) 43.
• 14. A. Simić, Electrochemical behavior and antioxidant and prooxidant activity of natural phenol, Molecules, 12 (2007) 2327.
• 15. M.D.P.T. Sotomayor, A.A. Tanaka, L.T. Kubota, Tris (2,2-bipyridil) copper (II) chloride complex: a biomimetic tyrosinase catalyst in the amperometric sensor construction, Electrochim. Acta, 48 (2003) 855.