Adsorption of BSA on Metal Loaded Monosize p GMA Microspheres

Year 2015, Volume: 43 Issue: 3, 225 - 233, 01.09.2015

Evrim Banu Altuntaş Fatma Kartal Deniz Türkmen Ali Derazshamshir Adil Denizli

Abstract

Immobilized metal ion affinity chromatography IMAC is an efficient method for the adsorption of histidine- having proteins so poly glycidyl methacrylate [p GMA ] monosize microspheres with an average diameter of 1.6 μm were produced by dispersion polymerization for adsorption of bovine serum albumin. Cu II ions were chelated on the poly glycidyl methacrylate -iminodiacetic acid [p GMA -IDA] microspheres to be used in bovine serum albumin adsorption studies in batch system. Characterizations of the microspheres were carried out by scanning electron microscopy SEM , elemental analysis, Fourier transform infrared FTIR spectroscopy. The maximum adsorption capacity of the p GMA -IDA-Cu II microspheres was found to be 278.6 mg/g polymer at pH 5.0. The elution studies were performed by 1.0 M NaCl solution. The obtained results showed that the metal chelated p GMA -IDA-Cu II microspheres can be evaluated as an efficicent adsorbent for albumin adsorption.

Keywords

Immobilized metal ion affinity chromatography, Albumin, Proteomics, Poly glycidyl methacrylate

Metal Yüklenmiş Eşboyutlu p GMA mikrokürelere BSA Adsorpsiyonu

Abstract

İ mmobilize metal iyon afinite kromatografi İMAK , histidin taşıyan proteinlerin adsorpsiyonu için etkili bir yöntem olduğundan sığır serum albuminin uzaklaştırılması için dispersiyon polimerizasyon yöntemiyle, 1.6 µm çapında eş boyutlu poli glisidil metakrilat [p GMA ] mikroküreler üretilmiştir. Cu II iyonları, kesikli sistemde sığır serum albuminin adsorpsiyon çalışmalarında kullanılmak üzere p GMA -IDA mikroküreleri ile şelatlanmıştır. Mikrokürelerin karakterizasyonu taramalı elektron mikroskobu SEM , elementel analiz, Fourier dönüşüm kızılötesi FTIR spektroskopisi kullanılarak gerçekleştirilmiştir. p GMA -IDA-Cu II mikrokürelerinin maksimum adsorpsiyon kapasitesi pH 5.0’te 278.6 mg/g polimer bulunmuştur. Elüsyon çalışmaları 1.0 M NaCl çözeltisi ile gerçekleştirilmiştir. Elde edilen sonuçlar p GMA -IDA-Cu II mikrokürelerin albüminin adsorpsiyonu için etkin bir adsorban olarak kabul edilebileceğini göstermiştir

Keywords

İmmobilize metal iyon afinite kromatografi, Albumin, Proteomik, Poli glisidil metakrilat

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