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Tümör nekroz faktörü-alfa tespiti için tek kullanımlık ITO-PET elektrot kullanan 11-(trietoksisilil) undekanal ajan bazlı biyosensör sistemi

Year 2023, Volume: 51 Issue: 3, 271 - 281, 16.07.2023
https://doi.org/10.15671/hjbc.1240299

Abstract

Bu çalışmada, TNF-α' nın insan serumunda tümör nekroz faktörünün saptanması için 11-(trietoksisilil) undekanal (11-TESU) ajanı ile modifiye edilmiş tek kullanımlık indiyum kalay oksit polietilen tereftalat (ITO-PET) elektroduna dayalı etiketsiz bir elektrokimyasal biyosensör sistemi geliştirilmiştir. Geliştirilen biyosensör, anti-TNF-α ve TNF-α arasındaki spesifik etkileşim için kullanılan elektrokimyasal empedans spektroskopisi (EIS), döngüsel voltammetri (CV) teknikleri, kare dalga voltametri (SWV) ve tek frekans empedans (SFI) tekniği ile gözlemlendi. Ek olarak, her bir ITO-PET yüzeyinin morfolojisinin nasıl değiştiğini (SEM) incelemek için taramalı elektron mikroskobu kullanıldı. 11-TESU konsantrasyonu, anti-TNF-α konsantrasyonu ve anti-TNF-α inkübasyon süresi gibi tüm parametreler optimize edildi. Biyosensör sistemi, lineer tayin aralığı, tekrarlanabilirlik, tekrar üretilebilirlik, rejenerasyon, depolama kararlılığı ve kaplanan yüzey alanı ölçülerek karakterize edildi. TNF-α elektrokimyasal biyosensör, yüksek tekrarlanabilirlik ve tekrar üretilebilirliğin yanı sıra geniş bir tayin aralığı (0,03 pg mL-1'den 3 pg mL-1'e) gösterdi. Biyosensörün LOD ve LOQ değerleri, sırasıyla 1x10-4 pg mL-1 ve 5x10-4 pg mL-1' dir. Önerilen biyosensörün klinik ortamlarda yararlı olup olmayacağını belirlemek için gerçek örneklere uygulandı.

Supporting Institution

TÜBİTAK

Project Number

113 Z 678

References

  • B. Aggarwal, C. S. Gupta, J. H. Kim, Historical perspectives on tumor necrosis factor and its superfamily: 25 years later, a golden journey, Blood, 119 (2012) 651–665.
  • P. M Sharif, P. Jabbari, S. Razi, M. K. Fathi, N. Rezaei, Importance of TNF-alpha and its alterations in the development of cancers, Cytokine, 130 (2020) 155066.
  • B. Yoon, Y. Yun, K. B. Kim, D. Kim, Inhibition of immunoproteasome attenuates NLRP3 inflammasome formation in tumor necrosis factor α-stimulated intestinal epithelial cell, Biochem. Biophys. Res. Commun., 624 (2022) 157-163.
  • S. Sri, D. Chauhan, G. B. V. S. Lakshmi, A. Thakar, P. R. Solanki, MoS2 nanoflower based electrochemical biosensor for TNF alpha detection in cancer patients, Electrochim. Acta., 405 (2022) 139-736.
  • S. L. Montgomery, W. J. Bowers, Tumor Necrosis Factor-alpha and the Roles it Plays in Homeostatic and Degenerative Processes Within the Central Nervous System, J. Neuroimmune Pharmacol., 7 (2012) 42–59.
  • C. Daniel, B. Oana, B. Ovidiu, B.N. Ioana, The dual role of tumour necrosis factor-alpha (TNF-α) in breast cancer: molecular insights and therapeutic approaches, Cell Oncol., 43 (2020) 1–18.
  • S. Keshav, L. Lawson, L.P. Chung, M. Stein, V.H. Perry, S. Gordon, Tumor necrosis factor mRNA localized to Paneth cells of normal murine intestinal epithelium by in situ hybridization, J. Exp. Med., 171 (1990) 327–332.
  • S. Lala, Y.Ogura, C. Osborne, S. Y. Hor, A.Bromfield, S. Davies, O. Ogunbiyi, G. Nunez, S. Keshav, Crohn’s disease and the NOD2 gene: A role for paneth cells, Gastroenterology, 125 (2003) 47–57.
  • Y. Zhao, T. Zhang, X. Shen, A. Huang, H. Li, L. Wang, X. Liu, X. Wang, X. Song, S. Wang, J. Dong, N. Shao, Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities, Mol. Ther., 30 (2022) 3052-3065.
  • M. Akgün, M.K. Sezgintürk, A novel biosensing system based on ITO-single use electrode for highly sensitive analysis of VEGF, Int J Environ Anal Chem.,100 (2020) 432-450.
  • Z. H. Khan, Effect of ITO surface properties on SAM modification: A review toward biosensor application, Cogent Eng., 3 (2016) 1170097.
  • O. J. Wahab, M. Kang, G. N. Meloni, E. Daviddi, P. R. Unwin, Nanoscale Visualization of Electrochemical Activity at Indium Tin Oxide Electrodes, Anal. Chem., 94 (2022) 4729–4736.
  • B. Demirbakan, M.K. Sezgintürk, A novel immunosensor based on fullerene C60 for electrochemical analysis of heat shock protein 70, J. Electroanal. Chem., 783 (2015) 201-207.
  • B. Demirbakan, B. Özcan, Ş. G. Yeşiller, M.K. Sezgintürk, Introducing a new method for evaluation of the interaction between an antigen and an antibody: Single frequency impedance analysis for biosensing systems, Talanta, 125 (2014) 7-13.
  • L. Barhoumi, F. G. Bellagambi, F. M. Vivaldi, A. Baraket, Y. Clément , N. Zine, M. B. Ali, A. Elaissari, A. Errachid, Ultrasensitive Immunosensor Array for TNF-α Detection in Artificial Saliva using Polymer-Coated Magnetic Microparticles onto Screen-Printed Gold Electrode, Sensors, 16 (2019) 692.
  • M. Bahri, A. Baraket, N. Zine, M. B. Ali, J. Bausells, E. Errachid, Capacitance electrochemical biosensor based on silicon nitride transducer for TNF-α cytokine detection in artificial human saliva: Heart failure (HF), Talanta, 209 (2020) 120501.
  • G. Baydemir, F. Bettazzi, I. Palchetti, D. Voccia, Strategies for the development of an electrochemical bioassay for TNF-alpha detection by using a non-immunoglobulin bioreceptor, Talanta, 151 (2016) 141-147.
  • R. Say, E. Birlik, Ö. Bic, U. Deniz, Nano anti-tumor necrosis factor-alpha based potentiometric sensor for tumor necrosis factor-alpha detection, Sens. Actuators B Chem., 209 (2015) 864-869.
  • A. Longo, A. Baraket, M. Vatteroni, N. Zine, J. Baussells, R. Fuoco, F. Di Francesco, G.S. Karanasiou, D. I. Fotiadis, A. Menciassi, A. Errachid, Highly sensitive electrochemical BioMEMS for TNF-α detection in human saliva: heart failure, Procedia Eng., 168 (2016) 97-100.
  • L. Barhoumi, A. Baraket, F.G. Bellagambi, G.S. Karanasiou, M. Ben Ali, D.I. Fotiadis, J. Bausells, N. Zine, M. Sigaud, A. Errachid, A novel chronoamperometric immunosensor for rapid detection of TNF-α in human saliva, Sens. Actuators B Chem., 266 (2018) 477-484.
  • R. Pruna, A. Baraket, A. Bonhomme, N. Zine, A. Errachid, M. Lopez, Novel nanostructured indium tin oxide electrode for electrochemical immunosensors: suitability for the detection of TNF-α. Electrochim. Acta., 283 (2018) 1632-1639.
  • S.R. Mahmoodi, P. Xie, M. Allen, M. Javanmard, Multiwell plate impedance analysis of a Nanowell Array sensor for label-free detection of cytokines in mouse serum, IEEE Sensors Lett., 4 (2020) 1-4.

11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection

Year 2023, Volume: 51 Issue: 3, 271 - 281, 16.07.2023
https://doi.org/10.15671/hjbc.1240299

Abstract

In this study, a label-free electrochemical biosensor system based on a disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrode modified with the 11-(triethoxysilyl) undecanal (11-TESU) agent was developed for the detection of tumour necrosis factor-alpha (TNF-α) in serum. The developed biosensor was observed with electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) techniques, square wave voltammetry (SWV) and single frequency impedance (SFI) technique which is utilized for the specific interaction between anti-TNF-α and TNF-α antigen. In addition, scanning electron microscopy was used to look at how the morphology of each ITO-PET surface changed (SEM). All parameters such as 11-TESU concentration, anti-TNF-α concentration and anti-TNF-α incubation time, were optimized. The biosensor system was characterized by measuring its linear determination range, repeatability, reproducibility, reusability, storage stability, and surface coverage. The TNF-αelectrochemical biosensor showed high levels of repeatability and reproducibility as well as a large dynamic range of detection (from 0.03 pg mL-1 to 3 pg mL-1). The LOD and LOQ for the biosensor were extremely low at 1x10-4 pg mL-1 and 5x10-4 pg mL-1, respectively. It was applied to real samples to determine whether the proposed biosensor would be useful in clinical settings.

Project Number

113 Z 678

References

  • B. Aggarwal, C. S. Gupta, J. H. Kim, Historical perspectives on tumor necrosis factor and its superfamily: 25 years later, a golden journey, Blood, 119 (2012) 651–665.
  • P. M Sharif, P. Jabbari, S. Razi, M. K. Fathi, N. Rezaei, Importance of TNF-alpha and its alterations in the development of cancers, Cytokine, 130 (2020) 155066.
  • B. Yoon, Y. Yun, K. B. Kim, D. Kim, Inhibition of immunoproteasome attenuates NLRP3 inflammasome formation in tumor necrosis factor α-stimulated intestinal epithelial cell, Biochem. Biophys. Res. Commun., 624 (2022) 157-163.
  • S. Sri, D. Chauhan, G. B. V. S. Lakshmi, A. Thakar, P. R. Solanki, MoS2 nanoflower based electrochemical biosensor for TNF alpha detection in cancer patients, Electrochim. Acta., 405 (2022) 139-736.
  • S. L. Montgomery, W. J. Bowers, Tumor Necrosis Factor-alpha and the Roles it Plays in Homeostatic and Degenerative Processes Within the Central Nervous System, J. Neuroimmune Pharmacol., 7 (2012) 42–59.
  • C. Daniel, B. Oana, B. Ovidiu, B.N. Ioana, The dual role of tumour necrosis factor-alpha (TNF-α) in breast cancer: molecular insights and therapeutic approaches, Cell Oncol., 43 (2020) 1–18.
  • S. Keshav, L. Lawson, L.P. Chung, M. Stein, V.H. Perry, S. Gordon, Tumor necrosis factor mRNA localized to Paneth cells of normal murine intestinal epithelium by in situ hybridization, J. Exp. Med., 171 (1990) 327–332.
  • S. Lala, Y.Ogura, C. Osborne, S. Y. Hor, A.Bromfield, S. Davies, O. Ogunbiyi, G. Nunez, S. Keshav, Crohn’s disease and the NOD2 gene: A role for paneth cells, Gastroenterology, 125 (2003) 47–57.
  • Y. Zhao, T. Zhang, X. Shen, A. Huang, H. Li, L. Wang, X. Liu, X. Wang, X. Song, S. Wang, J. Dong, N. Shao, Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities, Mol. Ther., 30 (2022) 3052-3065.
  • M. Akgün, M.K. Sezgintürk, A novel biosensing system based on ITO-single use electrode for highly sensitive analysis of VEGF, Int J Environ Anal Chem.,100 (2020) 432-450.
  • Z. H. Khan, Effect of ITO surface properties on SAM modification: A review toward biosensor application, Cogent Eng., 3 (2016) 1170097.
  • O. J. Wahab, M. Kang, G. N. Meloni, E. Daviddi, P. R. Unwin, Nanoscale Visualization of Electrochemical Activity at Indium Tin Oxide Electrodes, Anal. Chem., 94 (2022) 4729–4736.
  • B. Demirbakan, M.K. Sezgintürk, A novel immunosensor based on fullerene C60 for electrochemical analysis of heat shock protein 70, J. Electroanal. Chem., 783 (2015) 201-207.
  • B. Demirbakan, B. Özcan, Ş. G. Yeşiller, M.K. Sezgintürk, Introducing a new method for evaluation of the interaction between an antigen and an antibody: Single frequency impedance analysis for biosensing systems, Talanta, 125 (2014) 7-13.
  • L. Barhoumi, F. G. Bellagambi, F. M. Vivaldi, A. Baraket, Y. Clément , N. Zine, M. B. Ali, A. Elaissari, A. Errachid, Ultrasensitive Immunosensor Array for TNF-α Detection in Artificial Saliva using Polymer-Coated Magnetic Microparticles onto Screen-Printed Gold Electrode, Sensors, 16 (2019) 692.
  • M. Bahri, A. Baraket, N. Zine, M. B. Ali, J. Bausells, E. Errachid, Capacitance electrochemical biosensor based on silicon nitride transducer for TNF-α cytokine detection in artificial human saliva: Heart failure (HF), Talanta, 209 (2020) 120501.
  • G. Baydemir, F. Bettazzi, I. Palchetti, D. Voccia, Strategies for the development of an electrochemical bioassay for TNF-alpha detection by using a non-immunoglobulin bioreceptor, Talanta, 151 (2016) 141-147.
  • R. Say, E. Birlik, Ö. Bic, U. Deniz, Nano anti-tumor necrosis factor-alpha based potentiometric sensor for tumor necrosis factor-alpha detection, Sens. Actuators B Chem., 209 (2015) 864-869.
  • A. Longo, A. Baraket, M. Vatteroni, N. Zine, J. Baussells, R. Fuoco, F. Di Francesco, G.S. Karanasiou, D. I. Fotiadis, A. Menciassi, A. Errachid, Highly sensitive electrochemical BioMEMS for TNF-α detection in human saliva: heart failure, Procedia Eng., 168 (2016) 97-100.
  • L. Barhoumi, A. Baraket, F.G. Bellagambi, G.S. Karanasiou, M. Ben Ali, D.I. Fotiadis, J. Bausells, N. Zine, M. Sigaud, A. Errachid, A novel chronoamperometric immunosensor for rapid detection of TNF-α in human saliva, Sens. Actuators B Chem., 266 (2018) 477-484.
  • R. Pruna, A. Baraket, A. Bonhomme, N. Zine, A. Errachid, M. Lopez, Novel nanostructured indium tin oxide electrode for electrochemical immunosensors: suitability for the detection of TNF-α. Electrochim. Acta., 283 (2018) 1632-1639.
  • S.R. Mahmoodi, P. Xie, M. Allen, M. Javanmard, Multiwell plate impedance analysis of a Nanowell Array sensor for label-free detection of cytokines in mouse serum, IEEE Sensors Lett., 4 (2020) 1-4.
There are 22 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Burçak Demirbakan 0000-0002-8186-0461

Project Number 113 Z 678
Early Pub Date July 14, 2023
Publication Date July 16, 2023
Acceptance Date April 10, 2023
Published in Issue Year 2023 Volume: 51 Issue: 3

Cite

APA Demirbakan, B. (2023). 11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection. Hacettepe Journal of Biology and Chemistry, 51(3), 271-281. https://doi.org/10.15671/hjbc.1240299
AMA Demirbakan B. 11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection. HJBC. July 2023;51(3):271-281. doi:10.15671/hjbc.1240299
Chicago Demirbakan, Burçak. “11-(triethoxysilyl) Undecanal Agent-Based Biosensor System Using Disposable ITO-PET Electrode for Tumour Necrosis Factor-Alpha Detection”. Hacettepe Journal of Biology and Chemistry 51, no. 3 (July 2023): 271-81. https://doi.org/10.15671/hjbc.1240299.
EndNote Demirbakan B (July 1, 2023) 11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection. Hacettepe Journal of Biology and Chemistry 51 3 271–281.
IEEE B. Demirbakan, “11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection”, HJBC, vol. 51, no. 3, pp. 271–281, 2023, doi: 10.15671/hjbc.1240299.
ISNAD Demirbakan, Burçak. “11-(triethoxysilyl) Undecanal Agent-Based Biosensor System Using Disposable ITO-PET Electrode for Tumour Necrosis Factor-Alpha Detection”. Hacettepe Journal of Biology and Chemistry 51/3 (July 2023), 271-281. https://doi.org/10.15671/hjbc.1240299.
JAMA Demirbakan B. 11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection. HJBC. 2023;51:271–281.
MLA Demirbakan, Burçak. “11-(triethoxysilyl) Undecanal Agent-Based Biosensor System Using Disposable ITO-PET Electrode for Tumour Necrosis Factor-Alpha Detection”. Hacettepe Journal of Biology and Chemistry, vol. 51, no. 3, 2023, pp. 271-8, doi:10.15671/hjbc.1240299.
Vancouver Demirbakan B. 11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection. HJBC. 2023;51(3):271-8.

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