Year 2025,
Volume: 53 Issue: 2, 97 - 106, 01.04.2025
Seçil Kaya
,
Doğuşbey Ulusoy
,
Mahmut Emin Bodur
,
Onur Tokmak
Sema Yiyit Doğan
,
Ebru Kondolot Solak
Project Number
1919B012220863
References
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- A. F. Yesilsu, E. Alp-Erbay, L. N. Kahyaoğlu, İ. Aydin, Fish Waste to Valuable Products: Nanotechnological Approach, BioNanoScience., (2024) 1-19.
- J. Chen, M. Jayachandran, W. Bai, B. Xu, A critical review on the health benefits of fish consumption and its bioactive constituents, Food Chemistry, 369 (2022) 130874.
- M. Daviglus, J. Sheeshka, & E. Murkin, Health benefits from eating fish, Comments Toxicol., 8(4-6) (2002) 345-374.
- C. Krittanawong, A. Isath, J. Hahn, Z. Wang, B. Narasimhan, S. L. Kaplin, H. Jneid, S.S. Virani, W.H.W. Tang, Fish consumption and cardiovascular health: a systematic review, Am. J. Med., 134(6) (2021) 713-720.
- C. Pruneti, S. Guidotti, Need for Multidimensional and Multidisciplinary Management of Depressed Preadolescents and Adolescents: A Review of Randomized Controlled Trials on Oral Supplementations (Omega-3, Fish Oil, Vitamin D3), Nutr., 15(10) (2023) 2306.
- M. Sprague, T.C. Chau, D.I. Givens, Iodine content of wild and farmed seafood and its estimated contribution to UK dietary iodine intake, Nutr., 14(1), (2021), 195.
- J. A, Emery, F. Norambuena, J. Trushenski, G. M. Turchini, Uncoupling EPA and DHA in fish nutrition: dietary demand is limited in Atlantic salmon and effectively met by DHA alone, Lipids., 51(4), (2016), 399-412.
- H. Jafari, A. Lista, M. M. Siekapen, P. Ghaffari-Bohlouli, L. Nie, H. Alimoradi, A. Shavandi, Fish collagen: extraction, characterization, and applications for biomaterials engineering, Polym., 12(10), (2020) 2230.
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- G. Salihoglu, N. K. Salihoglu, S. Ucaroglu, M. Banar, Food loss and waste management in Turkey, Bioresour. Technol., 248 (2018) 88-99.
- Y. Xu, M. Bajaj, R. Schneider, S.L. Grage, A.S. Ulrich, J. Winter, C. Gallert, Transformation of the matrix structure of shrimp shells during bacterial deproteination and demineralization, Microb. cell fact., 12(1) (2013) 1-12.
- C. Campanati, D. Willer, J. Schubert, D.C. Aldridge, Sustainable intensification of aquaculture through nutrient recycling and circular economies: more fish, less waste, blue growth, Rev. Fish. Sci. Aquac., 30(2) (2022) 143-169.
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- A. Sionkowska, K. Adamiak, K. Musiał, M. Gadomska, Collagen based materials in cosmetic applications: A review, Mater., 13(19) (2020) 4217.
- I.N. Amirrah, Y. Lokanathan, I. Zulkiflee, M.M.R. Wee, A. Motta, M.B. Fauzi, A comprehensive review on collagen type I development of biomaterials for tissue engineering: From biosynthesis to bioscaffold, Biomedicines., 10(9) (2022) 2307.
- Y. Li, Y. Liu, R. Li, H. Bai, Z. Zhu, L. Zhu, C. Zhu, Z. Che, H. Liu, J. Wang, L. Huang, Collagen-based biomaterials for bone tissue engineering, Mater. Des., 210 (2021) 110049.
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- Indriani, S., Benjakul, S., Kishimura, H., Karnjanapratum, S., & Nalinanon, S. (2022). Impact of extraction condition on the yield and molecular characteristics of collagen from Asian bullfrog (Rana tigerina) skin. LWT. 162, 113439.
- Schmidt, M. M., Vidal, A. R., Mello, R. O., Mazutti, M. A., Cansian, R. L., Dornelles, R. C., ... & Kubota, E. H. (2021). Ultrasound as an alternative method to increase the extraction yield from chicken mecanically separated meatresidue collagen. J. Food Sci. Technol., 58, 2487-2496.
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- Z. Rajabimashhadi, N. Gallo, L. Salvatore, F. Lionetto, Collagen derived from fish industry waste: progresses and challenges, Polym., 15(3) (2023) 544.
- A. Shavandi, Y. Hou, A. Carne, M. McConnell, A.E.D.A. Bekhit, Marine waste utilization as a source of functional and health compounds, Adv. Food Nutr. Res., 87 (2019) 187-254.
- F. Subhan, Z. Hussain, I. Tauseef, A. Shehzad, F. Wahid, A review on recent advances and applications of fish collagen, Crit. Rev. Food Sci. Nutr., 61(6) (2021) 1027-1037.
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- E.B. Slimane, S. Sadok, Collagen from cartilaginous fish by-products for a potential application in bioactive film composite, Mar. Drugs, 16(6) (2018) 211.
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- R.A. Nazeer, R. Kavitha, R.J. Ganesh, S.Y. Naqash, N.S. Kumar, R. Ranjith, Detection of collagen through FTIR and HPLC from the body and foot of Donax cuneatus Linnaeus 1758, J. Food Sci. Technol., 51 (2014) 750-755.
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- P. Kulkarni, M. Maniyar, M. Nalawade, P. Bhagwat, S. Pillai, Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen, Environ. Sci. Pollut. Res., (2022) 1-11.
- L. He, W. Lan, Y. Wang, S. Ahmed, Y. Liu, Extraction and characterization of self-assembled collagen isolated from grass carp and crucian carp, Foods, 8(9) (2019) 396.
- C.H. Hsieh, C.Y. Shiau, Y.C. Su, Y.H. Liu, Y.R. Huang, Isolation and characterization of collagens from the skin of giant grouper (Epinephelus lanceolatus), J. Aquat. Food Prod., 25(1) (2016) 93-104.
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Sustainable Collagen Extraction from Fish Bones: A Green Solution for Waste Management
Year 2025,
Volume: 53 Issue: 2, 97 - 106, 01.04.2025
Seçil Kaya
,
Doğuşbey Ulusoy
,
Mahmut Emin Bodur
,
Onur Tokmak
Sema Yiyit Doğan
,
Ebru Kondolot Solak
Abstract
The global food industry's exponential growth has made it crucial to assess food waste. In this study, collagen extraction from waste fish bones was carried out in the presence of 0.5 M acetic acid. The physicochemical properties of the successfully obtained collagen were determined by Ultraviolet and Visible Spectroscopy (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), and Differential Scanning Calorimetry (DSC). The results of UV-Vis Spectra of collagen showed maximum absorption at 238 nm. The extracted collagen was found to have a triple helix structure by UV-Vis and FTIR analysis. It was determined that it was semi-crystalline with the XRD diffraction pattern. The thermal denaturation temperature was between 129 ºC and 141 ºC with a flow rate of -4.881 mW (141 ºC) and the enthalpy change (ΔH) was 39.2 mJ/mg. The study has shown that sufficient collagen can be isolated from fish waste simply and inexpensively. Moreover, the present study found that collagen obtained from fish processing waste can be used as a high-value-added material in many areas for various industrial purposes, such as the cosmetics and pharmaceutical industries. Most importantly, processing waste can be utilized, and environmental pollution can be prevented.
Project Number
1919B012220863
Thanks
This study, titled "Obtaining Collagen from Waste Fishbones for Sustainable Development, Its Characterization, and Production of a Product for the Cosmetic Industry," was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK) under Project Number 1919B012220863 within the framework of the 2209-A Research Project Support Programme for Undergraduate Students (2022/2). We extend our sincere gratitude to TÜBİTAK 2209-A for their support. We also gratefully acknowledge the facilities and assistance provided by the Central Research Laboratory Application and Research Center (MERLAB) at Ankara Yıldırım Beyazıt University.
References
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- A. F. Yesilsu, E. Alp-Erbay, L. N. Kahyaoğlu, İ. Aydin, Fish Waste to Valuable Products: Nanotechnological Approach, BioNanoScience., (2024) 1-19.
- J. Chen, M. Jayachandran, W. Bai, B. Xu, A critical review on the health benefits of fish consumption and its bioactive constituents, Food Chemistry, 369 (2022) 130874.
- M. Daviglus, J. Sheeshka, & E. Murkin, Health benefits from eating fish, Comments Toxicol., 8(4-6) (2002) 345-374.
- C. Krittanawong, A. Isath, J. Hahn, Z. Wang, B. Narasimhan, S. L. Kaplin, H. Jneid, S.S. Virani, W.H.W. Tang, Fish consumption and cardiovascular health: a systematic review, Am. J. Med., 134(6) (2021) 713-720.
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- Indriani, S., Benjakul, S., Kishimura, H., Karnjanapratum, S., & Nalinanon, S. (2022). Impact of extraction condition on the yield and molecular characteristics of collagen from Asian bullfrog (Rana tigerina) skin. LWT. 162, 113439.
- Schmidt, M. M., Vidal, A. R., Mello, R. O., Mazutti, M. A., Cansian, R. L., Dornelles, R. C., ... & Kubota, E. H. (2021). Ultrasound as an alternative method to increase the extraction yield from chicken mecanically separated meatresidue collagen. J. Food Sci. Technol., 58, 2487-2496.
- He, L., Lan, W., Zhao, Y., Chen, S., Liu, S., Cen, L., ... & Liu, Y. (2020). Characterization of biocompatible pig skin collagen and application of collagen-based films for enzyme immobilization. RSC Adv., 10(12), 7170-7180.
- S. Gaikwad, M.J. Kim, Fish By-Product Collagen Extraction Using Different Methods and Their Application, Mar. Drugs, 22(2) (2024) 60.
- M.V. Bhuimbar, P.K. Bhagwat, P.B. Dandge, Extraction and characterization of acid soluble collagen from fish waste: Development of collagen-chitosan blend as food packaging film, Journal of Environmental Chemical Engineering, 7(2) (2019) 102983.
- Z. Rajabimashhadi, N. Gallo, L. Salvatore, F. Lionetto, Collagen derived from fish industry waste: progresses and challenges, Polym., 15(3) (2023) 544.
- A. Shavandi, Y. Hou, A. Carne, M. McConnell, A.E.D.A. Bekhit, Marine waste utilization as a source of functional and health compounds, Adv. Food Nutr. Res., 87 (2019) 187-254.
- F. Subhan, Z. Hussain, I. Tauseef, A. Shehzad, F. Wahid, A review on recent advances and applications of fish collagen, Crit. Rev. Food Sci. Nutr., 61(6) (2021) 1027-1037.
- T. Nagai, N. Suzuki, N. Preparation and characterization of several fish bone collagens, J. Food Biochem., 24(5) (2000) 427-436.
- S.Z. Ramle, S.N.H. Oslan, R. Shapawi, R.A.M. Mokhtar, W.N.M. Noordin, N. Huda, Biochemical characteristics of acid-soluble collagen from food processing by-products of needlefish skin (Tylosurus acus melanotus), Appl. Sci., 12(24) (2022) 12695.
- A.G.D.M. Ampitiya, S.T. Gonapinuwala, C.A.N. Fernando, M.D.S.T. De Croos, Extraction and characterisation of type I collagen from the skin offcuts generated at the commercial fish processing centres, J. Food Technol., 60(2) (2023) 484-493.
- E.B. Slimane, S. Sadok, Collagen from cartilaginous fish by-products for a potential application in bioactive film composite, Mar. Drugs, 16(6) (2018) 211.
- T. Tanaka, K. Takahashi, K. Tsubaki, M. Hirata, K. Yamamoto, A. Biswas, T. Moriyama, Y. Kawamura, Isolation and characterization of acid-soluble bluefin tuna (Thunnus orientalis) skin collagen, Fish Aquat. Sci., 21 (2018) 1-8.
- M. Gauza-Włodarczyk, L. Kubisz, D. Włodarczyk, Amino acid composition in determination of collagen origin and assessment of physical factors effects, Int. J. Biol. Macromol., 104 (2017) 987-991.
- R.A. Nazeer, R. Kavitha, R.J. Ganesh, S.Y. Naqash, N.S. Kumar, R. Ranjith, Detection of collagen through FTIR and HPLC from the body and foot of Donax cuneatus Linnaeus 1758, J. Food Sci. Technol., 51 (2014) 750-755.
- A. Veeruraj, M. Arumugam, T. Ajithkumar, T. Balasubramanian, Isolation and characterization of collagen from the outer skin of squid (Doryteuthis singhalensis), Food Hydrocoll., 43 (2015) 708-716.
- P. Kulkarni, M. Maniyar, M. Nalawade, P. Bhagwat, S. Pillai, Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen, Environ. Sci. Pollut. Res., (2022) 1-11.
- L. He, W. Lan, Y. Wang, S. Ahmed, Y. Liu, Extraction and characterization of self-assembled collagen isolated from grass carp and crucian carp, Foods, 8(9) (2019) 396.
- C.H. Hsieh, C.Y. Shiau, Y.C. Su, Y.H. Liu, Y.R. Huang, Isolation and characterization of collagens from the skin of giant grouper (Epinephelus lanceolatus), J. Aquat. Food Prod., 25(1) (2016) 93-104.
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- R. Duan, J. Zhang, X. Du, X. Yao, K. Konno, Properties of collagen from skin, scale and bone of carp (Cyprinus carpio), Food Chem., 112(3) (2009) 702-706.
- H. Li, B.L. Liu, L.Z. Gao, H.L. Chen, Studies on bullfrog skin collagen, Food Chem., 84(1) (2004) 65-69.
- M. Yan, B. Li, X. Zhao, G. Ren, Y. Zhuang, H. Hou, X. Zhang, L. Chen, Y. Fan, Characterization of acid-soluble collagen from the skin of walleye pollock (Theragra chalcogramma), Food Chem., 107(4) (2008) 1581-1586.
- J.H. Muyonga, C.G.B. Cole, K.G. Duodu, Characterisation of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus), Food Chem., 85(1) (2004) 81-89.
- Y. Abe, S. Krimm, Normal vibrations of crystalline polyglycine I, Biopolymers., 11(9) (1972) 1817-1839.
- W. Wei, Y. Yan, X.P. Zhang, Y. Liu, Y. Lu, W.Z. Shi, C.H. Xu, Enhanced chemical and spatial recognition of fish bones in surimi by Tri-step infrared spectroscopy and infrared microspectroscopic imaging, Spectrochim. Acta A Mol. Biomol. Spectrosc., 205 (2018) 186-192.
- Z. Movasaghi, S. Rehman, I.U. Rehman, Fourier transform infrared (FTIR) spectroscopy of biological tissues, Appl. Spectrosc. Rev., 43(2) (2008) 134-179.
- T. Riaz, R. Zeeshan, F. Zarif, K. Ilyas, N. Muhammad, S.Z. Safi, A. Rahim, S.A.A. Rizvi, I.U. Rehman, FTIR analysis of natural and synthetic collagen, Appl. Spectrosc. Rev., 53(9) (2018) 703-746.
- R. Sripriya, R. Kumar, A novel enzymatic method for preparation and characterization of collagen film from swim bladder of fish Rohu (Labeo rohita), Food Nutr. Sci., 6(15) (2015) 1468-1478.
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