Pentilentetrazol Epilepsi Modelinde Racine Skorlama Sistemine Yeni Bir Bakış

Abstract

Amaç: Pentilentetrazol (PTZ), antiepileptik ilaçlar geliştirmek ve epilepsi mekanizmasını araştırmak için çalışmalarda ağırlıklı olarak kullanılan kimyasaldır. PTZ sistematik olarak verildiğinden, ilk önce miyoklonik kasılmalar yaratarak genelleşmiş tonik-klonik nöbetleri takiben genel nöbetlere yol açar. Epilepsi tutuşma modeline göre, hayvanlara tekrarlanan kimyasal ile stimülasyon, lokal dokuda elektrofizyolojik değişikliklere neden olur.
Materyal ve Metod: Çalışma dişi (n=7) ve erkek (n=7) Wistar-Albino sıçanlarına intraperitoneal PTZ uygulaması gerçekleştirildi. İki günde bir 35 mg/kg'lık bir dozda uygulanan PTZ ajanı, modelin 1 ayın sonunda 50 mg/kg'lık son dozda tutuşmasını sağladı. Nöbetler Racine skorlama sistemine göre puanlandı ve videoya kaydedildi. Fazlar arasına ara-faz seviyeleri eklenerek deney hayvanlarının nöbetlerinin daha iyi gözlemlenmesi ve puanlandırılması amaçlanmıştır.
Bulgular: Sonuçlara göre hem dişi hem de erkek epilepsi gruplarında önceki Racine skorlama sistemindeki analize göre anlamlı bir fark vardı.
Sonuç: Geliştirilen yöntemin önemi, hayvan modelinin tüm derecelerine ara seviyeler ekleyerek daha geçerli ve kullanışlı bir model belirlemektir.

Keywords

• kindled
• epilepsy model
• rats
• Racine

A New View of The Racine Scoring System in The Pentylenetetrazol Epilepsy Model

Abstract

Background: Pentylenetetrazol (PTZ) is the predominantly used chemical in studies to develop antiepileptic drugs and to investigate the mechanism of epilepsy. As PTZ is given systematically, it leads to generalized seizures, first creating myoclonic contractions and following generalized tonic-clonic seizures. According to the kindling model of epilepsy, repeated chemical stimulation to animals causes electrophysiological changes in local tissue. It was aimed to better observe and score the seizures of experimental animals by placing intermediate-phase levels between the phases.
Materials and Methods: The study was performed by intraperitoneal administration of PTZ to female (n=7) and male (n=7) Wistar-Albino rats. PTZ agent administered at a dose of 35 mg/kg every two days allowed the model to kindle at a final dose of 50 mg/kg at the end of one month. The seizures were scored according to the Racine scoring method and recorded on video.
Results: According to the results, there was a significant difference between phase values of original Racine scoring and those of new suggested Racine scoring for both female and male epilepsy groups
Conclusions: The importance of the developed method is to determine a more valid and more useful model by adding intermediate levels to all grades of the animal model proposing to change the degree of scoring in the literature.

Keywords

• Kindled
• Epilepsy model
• Rats
• Racine

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