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Production Of Tetracycline Hydrochloride-Collagen-Doped Chitosan Nanofiber Scaffolds And Investigation Of Their Antibacterial Properties

Year 2023, , 37 - 60, 15.04.2023
https://doi.org/10.38001/ijlsb.1180026

Abstract

The aim of the study is to develop a noble biomaterial that can accelerate the healing process without the risk of infection by loading tetracycline hydrochloride and collagen hemostatic agent into the chitosan tissue scaffold. After the trials, a good tissue scaffold was obtained from chitosan and PVA by electro spinning. To increase the hemostatic features of this biomaterial, 10% (by weight) collagen hemostatic agent was added to the PVA chitosan blend. After the amount of collagen hemostatic agent in the chitosan tissue scaffold was set, various amounts of tetracycline were added and 5 different biomaterials were developed to augment the antibacterial and wound healing properties. Antibiotic concentration in the biomaterial was IV 10% in the first, 15% in the second, 20% in the third, 25% in the fourth and 30% in the fifth sample. Finally, the effects of the obtained biomaterials on the nosocomial bacteria (gram positive: Staphylococcus Aureus, gram negative: Pseudomonas Aeruginosa) were analyzed with in-vitro tests at Kahramanmaras Sutcu Imam University, School of Medicine Department of Microbiology laboratories. As a result of the examination, it was examined how much the biomaterial should be and how effective it was against bacterial growth on the first, third and fifth days. It is thought that biomaterial will be very effective in emergencies and surgical procedures.

Supporting Institution

Kahramanmaraş Sütçü İmam University Scientific Research Projects Coordination Unit

Project Number

2015/1-50YLS

Thanks

This study was supported by Kahramanmaraş Sütçü İmam University Scientific Research Projects Coordination Unit (Project Number: 2015/1-50YLS). This study was partially presented as an oral presentation at the EURASIA 3rd International Congress of Applied Sciences symposium.

References

  • 1. Atrous, M., Torkia, Y.B., Selmi, T., Bouzid, M., Seffen, M., Lamine, B., Statistical physics treatment of tetracycline adsorption: energetic studies, Chemical Papers, 76, 4339-4339, 2022.
  • 2. Shen, Q., Xu, M.H., Wu, T., Pan, G.X., Tang, P.S., Adsorption behavior of tetracycline on carboxymethyl starch grafted magnetic bentonite, Chemical Papers, 76, 123-135, 2022.
  • 3. Sefidehkhan, Y.S., Khoshkam, M., Amiri, M., Chemometrics-assisted electrochemical determination of dextromethorphan hydrobromide and phenylephrine hydrochloride by carbon paste electrode, Chemical Papers, 75, 6565-6573, 2021.
  • 4. Aliabad, H.A.R., Chahkandi, M., Theoretical study of crystalline network and optoelectronic properties of erlotinib hydrochloride molecule: non-covalent interactions consideration, Chemical Papers, 73, 737-746, 2019.
  • 5. Gong, X., Yang, D., Wang, N., Sun, S., Nie, J., Ma, G., Fibers and Polymers, 21, 2231- 2238, 2019.
  • 6. Hosseini, S.M., Abdouss, M., Mazinani, S., Soltanabidi, A., Kalee, M., Modified nanofiber containing chitosan and graphene oxide-magnetite nanoparticles as effective materials for smart wound dressing, Composites Part B: Engineering, 231, 2022.
  • 7. Yıldırım, N., Küçük, İ., Preparing and characterization of St.John’s Wort (Hypericum perforatum) incorporated wound dressing films based on chitosan and gelatin, Journal of the Faculty of Engineering and Architecture of Gazi University, 35 (1), 127-135, 2020.
  • 8. Kouchak M., Handali S., Boroujeni B.N., Evaluation of the mechanical properties and drug permeability of Chitosan/Eudragit RL composite film, Osong Public Health Research Perspect, 6, 14-19, 2015.
  • 9. Kip, F., Açıkel, Ü., Removal of tetraclycine by biocomposites synthesized with immobilization of Rhizopus delamar and Candida types, Journal of the Faculty of Engineering and Architecture of Gazi University, 34 (3), 1417-1426, 2019.
  • 10. Pitarresi, G., Fiorica, C., Palumbo, F. S., Calascibetta, F., Giammona, G., Polyaspartamide-Polylactide Electrospun Scaffolds for Potential Topical Release of Ibuprofen, Journal of Biomedical Materials Research, 100, 1565–1572, 2012.
  • 11. Arıkan, H., İlaç Salınımlı Kompozit Yarabandı Tasarımı ve Üretimi Yüksek Lisans Tezi, Kahramanmaraş Sütçü İmam Ünversitesi Fen Bilimleri Enstitüsü, Kahramanmaraş, 2014.
  • 12. Taepaiboon, P., Rungsardthong, U., Supaphol, P., Drug-loaded electrospun mats of poly(vinyl alcohol) fibres and their release characteristics of four model drugs, Instıtute Of Physıcs Publıshıng Nanotechnology 17, 2317–2329,2006.
  • 13. Yu, D., Yu, j.,Chen, L.,Williams, G., Modified coaxial electrospinning for the preparation of high-quality ketoprofen-loaded cellulose acetate nanofibers, Carbohydrate Polymers,
  • 14. Zeng, J., Yang, L., Liang, Q., Zhang, X., Guan, H., Xu, X., Chen, X., Jing, X., Influence Of The Drug Compatibility With Polymer Solution On The Release Kin Etics Of Electrospun Fiber Formulation, Journal Of Controlled Release, 90 (2), 1016-1023, 2005.
  • 15. Lee, D.W., Powers, K. ve Baney, R., “Physicochemical Properties and Blood Compatibility of Acylated Chitosan Nanoparticles”, Carbohydrate Polymers, 58, 371-377, 2004.
  • 16. Coban A., Martin A., Uzun M., Bilgin K, Palomino JC., Durupinar B. Evaluation of blood agar for susceptibility testing of Mycobacterium tuberculosis against first-line antituberculous drugs: results from two centers. J Chemother; 20(3), 388-412, 2008

Production Of Tetracycline Hydrochloride-Collagen-Doped Chitosan Nanofiber Scaffolds And Investigation Of Their Antibacterial Properties

Year 2023, , 37 - 60, 15.04.2023
https://doi.org/10.38001/ijlsb.1180026

Abstract

Çalışmanın amacı kitosan doku iskelesine tetrasiklin hidroklorür ve kollajen hemostatik ajan yükleyerek enfeksiyon riski olmadan iyileşme sürecini hızlandırabilen asil bir biyomateryal geliştirmektir. Denemelerden sonra, elektro eğirme ile kitosan ve PVA'dan iyi bir doku iskelesi elde edildi. Bu biyomateryalin hemostatik özelliklerini arttırmak için, PVA kitosan karışımına %10 (ağırlıkça) kollajen hemostatik ajan eklendi. Kitosan doku iskelesindeki kollajen hemostatik ajan miktarı ayarlandıktan sonra çeşitli miktarlarda tetrasiklin eklenmiş ve antibakteriyel ve yara iyileştirici özelliklerini artırmak için 5 farklı biyomateryal geliştirilmiştir. Biyomateryaldeki antibiyotik konsantrasyonu birinci örnekte IV %10, ikinci örnekte %15, üçüncü örnekte %20, dördüncü örnekte %25 ve beşinci örnekte %30 idi. Son olarak, elde edilen biyomalzemelerin hastane bakterileri üzerindeki etkileri (gram pozitif: Staphylococcus Aureus, gram negatif: Pseudomonas Aeruginosa) Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Mikrobiyoloji Anabilim Dalı laboratuvarlarında in vitro testler ile analiz edilmiştir. İnceleme sonucunda birinci, üçüncü ve beşinci günlerde biyomalzemenin ne kadar olması gerektiği ve bakteri üremesine karşı ne kadar etkili olduğu incelenmiştir. Biyomateryalin acil durumlarda ve cerrahi işlemlerde çok etkili olacağı düşünülmektedir.

Project Number

2015/1-50YLS

References

  • 1. Atrous, M., Torkia, Y.B., Selmi, T., Bouzid, M., Seffen, M., Lamine, B., Statistical physics treatment of tetracycline adsorption: energetic studies, Chemical Papers, 76, 4339-4339, 2022.
  • 2. Shen, Q., Xu, M.H., Wu, T., Pan, G.X., Tang, P.S., Adsorption behavior of tetracycline on carboxymethyl starch grafted magnetic bentonite, Chemical Papers, 76, 123-135, 2022.
  • 3. Sefidehkhan, Y.S., Khoshkam, M., Amiri, M., Chemometrics-assisted electrochemical determination of dextromethorphan hydrobromide and phenylephrine hydrochloride by carbon paste electrode, Chemical Papers, 75, 6565-6573, 2021.
  • 4. Aliabad, H.A.R., Chahkandi, M., Theoretical study of crystalline network and optoelectronic properties of erlotinib hydrochloride molecule: non-covalent interactions consideration, Chemical Papers, 73, 737-746, 2019.
  • 5. Gong, X., Yang, D., Wang, N., Sun, S., Nie, J., Ma, G., Fibers and Polymers, 21, 2231- 2238, 2019.
  • 6. Hosseini, S.M., Abdouss, M., Mazinani, S., Soltanabidi, A., Kalee, M., Modified nanofiber containing chitosan and graphene oxide-magnetite nanoparticles as effective materials for smart wound dressing, Composites Part B: Engineering, 231, 2022.
  • 7. Yıldırım, N., Küçük, İ., Preparing and characterization of St.John’s Wort (Hypericum perforatum) incorporated wound dressing films based on chitosan and gelatin, Journal of the Faculty of Engineering and Architecture of Gazi University, 35 (1), 127-135, 2020.
  • 8. Kouchak M., Handali S., Boroujeni B.N., Evaluation of the mechanical properties and drug permeability of Chitosan/Eudragit RL composite film, Osong Public Health Research Perspect, 6, 14-19, 2015.
  • 9. Kip, F., Açıkel, Ü., Removal of tetraclycine by biocomposites synthesized with immobilization of Rhizopus delamar and Candida types, Journal of the Faculty of Engineering and Architecture of Gazi University, 34 (3), 1417-1426, 2019.
  • 10. Pitarresi, G., Fiorica, C., Palumbo, F. S., Calascibetta, F., Giammona, G., Polyaspartamide-Polylactide Electrospun Scaffolds for Potential Topical Release of Ibuprofen, Journal of Biomedical Materials Research, 100, 1565–1572, 2012.
  • 11. Arıkan, H., İlaç Salınımlı Kompozit Yarabandı Tasarımı ve Üretimi Yüksek Lisans Tezi, Kahramanmaraş Sütçü İmam Ünversitesi Fen Bilimleri Enstitüsü, Kahramanmaraş, 2014.
  • 12. Taepaiboon, P., Rungsardthong, U., Supaphol, P., Drug-loaded electrospun mats of poly(vinyl alcohol) fibres and their release characteristics of four model drugs, Instıtute Of Physıcs Publıshıng Nanotechnology 17, 2317–2329,2006.
  • 13. Yu, D., Yu, j.,Chen, L.,Williams, G., Modified coaxial electrospinning for the preparation of high-quality ketoprofen-loaded cellulose acetate nanofibers, Carbohydrate Polymers,
  • 14. Zeng, J., Yang, L., Liang, Q., Zhang, X., Guan, H., Xu, X., Chen, X., Jing, X., Influence Of The Drug Compatibility With Polymer Solution On The Release Kin Etics Of Electrospun Fiber Formulation, Journal Of Controlled Release, 90 (2), 1016-1023, 2005.
  • 15. Lee, D.W., Powers, K. ve Baney, R., “Physicochemical Properties and Blood Compatibility of Acylated Chitosan Nanoparticles”, Carbohydrate Polymers, 58, 371-377, 2004.
  • 16. Coban A., Martin A., Uzun M., Bilgin K, Palomino JC., Durupinar B. Evaluation of blood agar for susceptibility testing of Mycobacterium tuberculosis against first-line antituberculous drugs: results from two centers. J Chemother; 20(3), 388-412, 2008
There are 16 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

İbrahim Altun 0000-0002-9117-3518

İsmail Tiyek 0000-0002-1643-8977

Eda Altun 0000-0001-7916-0418

İdris Altun 0000-0003-4263-766X

Project Number 2015/1-50YLS
Publication Date April 15, 2023
Published in Issue Year 2023

Cite

EndNote Altun İ, Tiyek İ, Altun E, Altun İ (April 1, 2023) Production Of Tetracycline Hydrochloride-Collagen-Doped Chitosan Nanofiber Scaffolds And Investigation Of Their Antibacterial Properties. International Journal of Life Sciences and Biotechnology 6 1 37–60.


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