Year 2021, Volume 3, Issue 2, 121 - 128, 24.08.2021

Yiğit DEVECİ Gamze GÜNAL SADIK Emine AKALIN Seda KUŞOĞLU GÜLTEKİN Ayşegül YANIK Belkis ATASEVER ARSLAN

https://doi.org/10.38058/ijsl.983389

Abstract

Anti-diabetic Effects of Berberis cretica Extract in INS-1E Cells

Abstract

Berberine has been used for the adjuvant treatment of type 2 diabetes mellitus, hyperlipidemia (high levels of fats), and hypertension (high blood pressure). Also, it has different effects on diarrhea, inflammation, and cancer. Berberine, is a profoundly common compound in Berberis species. Although Berberis cretica is one of the Berberis species, it is unknown whether it has anti-diabetic effects yet. Also, synergistic effects of various compounds together with berberin or similar chemical forms of berberine within Berberis species can lead to find new anti-diabetic agents. The aim of this study is to investigate possible drug potential of Berberis cretica extract containing berberine and, its potential signaling pathways on Rat Insulinoma (INS-1E) cells. According to our results, Berberis cretica extract has anti-apoptotic effects in INS-1E cells decreasing expression p53, p38 and Bax genes. Suppressive effects of Berberis cretica plant extracts on apoptotic signalling pathways in β cells show that the extract contents can have a drug potential for treatment of diabetes.

Keywords

INS-1E, Apoptosis, Insulin, Berberis cretica

References

• Abrams, S. L., Follo, M. Y., Steelman, L. S., Lertpiriyapong, K., Cocco, L. et al. 2019. Abilities of berberine and chemically modified berberines to inhibit proliferation of pancreatic cancer cells, Advances in Biological Regulation, 71: 172-182.
• Alemardan, A., Asadi, W., Rezaei, M., Tabrizi, L., Mohammadi, S. 2013. Cultivation of Iranian seedless barberry (Berberis integerrima ‘Bidaneh’): A medicinal shrub, Industrial Crops and Products, 50: 276-287.
• Altinoz, E., Taskin, E., Oner, Z., Elbe, H., Atasever Arslan, B. 2015. The effect of saffron (Its active constituent, crocin) on The cardiovascular complication and dyslipidemia in streptozotocin induced diabetic rats, African Journal of Traditional, Complementary and Alternative Medicines, 12(5): 1-7.
• Altundağ, E. M., Becer, E., Şanlıtürk, G., Güran, M., Vatansever, H. S. 2020. Berberin-Klorürün meme kanseri hücreleri üzerindeki sitotoksik etkilerinin değerlendirilmesi ve antimikrobiyal aktivite analizi, Turkiye Klinikleri Journal of Medical Sciences, 40(3): 342-348.
• Chu, Q., Jiang, Y., Zhang, W., Xu, Du, W. et al., 2016. Pyroptosis is involved in the pathogenesis of human hepatocellular carcinoma, Oncotarget, 7: 84658-665.
• Duran-Salgado, M. B., Rubio-Guerra, A. F. 2014. Diabetic nephropathy and inflammation, World J Diabetes, 5(3): 393-398.
• Durmuş, R., Şahin, E., Bireller, S. 2016. Gestasyonel diyabette hipoglisemik etkili bitkilerin kullanımı, Deneysel Tıp Araştırma Enstitüsü Dergisi, 6(11): 3-16.
• Ferranini, E. 1998. Insulin resistance versus insulin deficiency in noninsulin dependent diabetes mellitus: Problems and prospects, Endocrine Reviews, 19: 447-458.
• İleritürk, M., Doğan, T., Kandemir, O. 2021. Investigation of the effect of berberine with arginase activity and oxidantantioxidant parameters on bortezomib-induced spleen injury in rats, Kocatepe Veterinary Journal, 14(1): 6-15.
• Kigili, F., Ozen, F., Catal, T., Atasever Arslan, B. 2019. Androgen receptor (NR3C4) regulator potential of Ceratonia siliqua extract and its signaling pathways, Pharmacognosy Magazine, 15(62): 1-4
• Kukula-Kocha, W., Aligiannis, N., Halabalaki, M., Skaltsounis, A. L., Glowniak, K., Kalpoutzakis, E. 2013. Influence of extraction procedures on phenolic content and antioxidant activity of cretan baberry herb, Food Chemistry, 138: 406-413.
• Lan, J., Zhao, Y., Dong, F., Yan, Z., Zheng, W. et al. 2015. Meta-analysis of the effect and safety of berberine in the treatment of type 2 Diabetes mellitus, hyperlipemia and hypertension, Journal of Ethnopharmacol, 161: 69-81.
• Li, H. L., Wu, H., Zhang, B. B., Shi, H.L., Wu, X.J. 2016. MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo, Molecular Medicine Reports, 14(2): 1430-1438.
• Li, J., He, W., Liao, B., Yang, J. 2015. FFA-ROS-P53-mediated mitochondrial apoptosis contributes to reduction of osteoblastogenesis and bone mass in type 2 diabetes mellitus, Scientific Reports, 31(5): 12724.
• Oltvai, Z. N., Milliman, C. L., Korsmeyer, S. J. 1993. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death, Cell, 74: 609-619.
• Onay-Beşikci, A., Güner, Ş. 2006. Diyabetik kalpte görülen mekanik ve metabolik değişimler ve bunlarin tedavisinde metabolik yaklaşım, Ankara Eczacılık Fakültesi Dergisi, 35(4): 297-317.
• Puthdee, N., Seubwai, W., Vaeteewoottacharn, K., Boonmars, T., Cha'on, U. et al. 2017. Berberine induces cell cycle arrest in cholangiocarcinoma cell lines via inhibition of NF-kappaB and STAT3 pathways, Biological & Pharmaceutical Bulletin, 40(6): 751-757.
• Rhodes, C. J. 2005. Type 2 diabetes-a matter of beta-cell life and death? Science, 307(5708): 380-384.
• Salcini, C., Atasever-Arslan, B., Sunter, G., Gur H., Isik, F. B. et al. 2016. High plasma pentraxin 3 levels in diabetic polyneuropathy patients with nociceptive pain, The Tohoku Journal of Experimental Medicine, 239(1): 73-79.
• Tabakoğlu, E., Durgut, R. 2013. Veteriner hekimlikte oksidatif stres ve bazı önemli hastalıklarda oksidatif stresin etkileri, Adana Veteriner Kontrol ve Araştırma Enstitüsü Dergisi, 3(1), 69-75.