Use of the radicle emergence test (RE) to estimate germination and emergence potential in sponge gourd (Luffa aegyptiaca Mill.) seed genotypes
Abstract
The study was carried out to test whether the radicle emergence (RE) test could be used to rank 12 Luffa (Luffa aegyptiaca Mill.) seed genotypes according to total (TG, %) and normal (NG, %) germination percentages in laboratory conditions (25°C, 14 days, dark), and mean germination time (MGT, day), seedling emergence percentage (SE, %), seedling shoot weight (SSW, g/plant) and hypocotyl thickness (HT, mm/plant) in climatic room conditions (23±2°C, 70% relative humidity), in peat moss medium after 15 days. RE (2 mm radicle) counts were made at 24, 48, 72, 96, 120 and 144 hours at 25°C in the dark after germination was set up. RE values were correlated to seed germination and seedling quality parameters. The highest correlation values occurred after 72h and onwards. RE 72h and seed quality parameters were regressed and were found to be significantly related to TG (R2=0.732, P<0.001), NG (R2=0.751, P<0.001), MGT (R2=0.842, P<0.001), SE (R2=0.754, P<0.001), SSW (R2=0.349, P<0.05), and HT (R2=0.757, P<0.001). The potential of the RE test to discriminate between Luffa genotypes according to their germination and emergence potential is discussed.
Keywords
Luffa genotypes, RE test, Germination, Seedling emergence, Seed vigour
References
- Demir, I., Ermis, S., Mavi, K. & Matthews, S. (2008). Mean germination time of pepper seed lots (Capsicum annuum L.) predicts size and uniformity of seedlings in germination tests and transplant modules. Seed Science and Technology, 36(1), 21-30.
- Demir, I., Özden, E., Gökdaş, Z., Njie, E. S. & Aydin, M. (2020). Radicle emergence test predicts normal germination percentages of onion seed lots with different cultivars and genotypes. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 25(3), 434-442.
- Eren, E., Ermis, S., Oktem, G. & Demir, I. (2023). Seed longevity potential predicted by radicle emergence (RE) vigor test in watermelon seed cultivars. Horticulturae, 9(2), 280.
- Ermis, S., Oktem, G., Mavi, K., Hay, F. R. & Demir, I. (2022). The radicle emergence test and storage longevity of cucurbit rootstock seed lots. Seed Science and Technology, 50(1), 1-10.
- ISTA (2022). International Rules for Seed Testing, International Seed Testing Association, Bassersdorf, Switzerland.
- Jang, Y., Moon, J. H., Kim, S. G., Kim, T., Lee, O. J., Lee, H. J. & Wi, S. H. (2022). Effect of low-temperature tolerant rootstocks on the growth and fruit quality of watermelon in semi-forcing and retarding culture. Agronomy, 13(1), 67.
- Khajeh-Hosseini, M., Lomholt, A. & Matthews, S. (2009). Mean germination time in the laboratory estimates the relative vigour and field performance of commercial seed lots of maize (Zea mays L.). Seed Science and Technology, 37(2), 446-456.
- Khajeh-Hosseini, M, Nasehzadeh, M. & Matthews, S. (2010). Rate of physiological germination relates to the percentage normal seedlings in standard germination tests of naturally aged seed lots of oilseed rape. Seed Science and Technology 38 (3), 602-611
- Lee, J. M., Kubota, C., Tsao, S. J., Bie, Z., Echevarria, P. H., Morra, L. & Oda, M. (2010). Current status of vegetable grafting: diffusion, grafting techniques, automation. Scientia Horticulturea, 127, 93-105. https://doi.org/10.1016/j.scienta.2010.08.003
- Li, H., Wang, F., Chen, X. J., Shi, K., Xia, X. J., Considine, M. J., Yu, J. Q. & Zhou, Y. H. (2014). The sub/supra-optimal temperature-induced inhibition of photosynthesis and oxidative damage in cucumber leaves are alleviated by grafting onto figleaf gourd/luffa rootstocks. Physiologia Plantarum, 152, 571–584.
