Research Article

Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions

Volume: 8 Number: 4 December 28, 2024
EN

Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions

Abstract

Drought stress is a significant threat to plant cultivation in arid and semi-arid regions, negatively affecting growth and leading to plant mortality. This study evaluated the in vitro drought tolerance of Aronia melanocarpa ‘Nero’ by exposing micropropagated plantlets to nutrient media containing different concentrations of PEG-8000 (0%, 1%, and 2%) during the rooting stage. Results showed that increasing PEG concentrations led to a reduction in survival, rooting, plantlet growth, and physiological parameters. The 0% PEG treatment resulted in the highest survival rate (95.83%), root number, and chlorophyll content, while the 2% PEG treatment significantly hindered these parameters. These findings indicate that Aronia melanocarpa 'Nero' is sensitive to drought stress, with reduced growth and physiological activity under higher PEG concentrations.

Keywords

Chlorophyll, Drought stress, In vitro, Micropropagation, Growth

References

  1. Albiski, F., Najla, S., Sanoubar, R., Alkabani, N. & Murshed, R. (2012). In vitro screening of potato lines for drought tolerance. Physiology and Molecular biology of plants, 18, 315-321. https://doi.org/10.1007/s12298-012-0127-5
  2. Almokar, H.M.M. & Pirlak, L. (2018). Propagation of Aronia (Aronia melanocarpa) with tissue culture. Selcuk Journal of Agriculture and Food Sciences, 32(3), 549-558. https://doi.org/10.15316/SJAFS.2018.136
  3. Altıncı, N.T. & Cangi, R. (2019). Drought Tolerance of Some Wine Grape Cultivars under In Vitro Conditions. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 36(2), 145-152. https://doi.org/10.13002/jafag4633
  4. Anjum, S.A., Xie, X.Y., Wang, L. C., Saleem, M.F., Man, C. & Lei, W. (2011). Morphological, physiological and biochemical responses of plants to drought stress. African journal of agricultural research, 6(9), 2026-2032. https://doi.org/10.5897/AJAR10.027
  5. Babu, G.A., Mosa Christas, K., Kowsalya, E., Ramesh, M., Sohn, S. I., & Pandian, S. (2022). Improved sterilization techniques for successful in vitro micropropagation. In Commercial Scale Tissue Culture for Horticulture and Plantation Crops (pp. 1-21). Singapore: Springer Nature Singapore.
  6. Bilir Ekbic, H., Gecene, İ. & Ekbic, E. (2022). Tilki Üzümlerinin (Vitis labrusca L.) Kuraklık Stresine Toleransının in vitro PEG Uygulaması ile Belirlenmesi. Erwerbs-Obstbau, 64 (Ek 1), 87-94 (in Turkish). https://doi.org/10.29278/azd.1059957
  7. Celik, H., Karabulut, B., & Uray, Y. (2022). Growth-Development, yield and quality characteristics of Aronia varieties grown in pots. International Journal of Innovative Approaches in Agricultural Research, 6(3), 246-254. https://doi.org/10.29329/ijiaar.2022.475.8
  8. Dobránszki, J., Magyar-Tábori, K., & Takács-Hudák, Á. (2003). Growth and developmental responses of potato to osmotic stress under in vitro conditions. Acta biologica hungarica, 54, 365-372.
  9. Ekinci, H., Saskin, N., Korkmaz, Ş., Ak, B.E. & Aydınlık, Y. (2024). The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13542-13542. https://doi.org/10.15835/nbha52113542
  10. Gecene, İ. (2020). Kokulu Üzümün (Vitis labrusca L.) Kuraklık Stresine Toleransının Peg Uygulamasıyla In Vitro Koşullarda Belirlenmesi. Ordu Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı, Yüksek Lisans Tezi, Ordu, Türkiye, 52 s.
APA
Ekinci, H., Şaşkın, N., Dikmetaş, B., & Ak, B. E. (2024). Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions. International Journal of Agriculture Environment and Food Sciences, 8(4), 760-767. https://doi.org/10.31015/jaefs.2024.4.4
AMA
1.Ekinci H, Şaşkın N, Dikmetaş B, Ak BE. Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions. int. j. agric. environ. food sci. 2024;8(4):760-767. doi:10.31015/jaefs.2024.4.4
Chicago
Ekinci, Heydem, Necla Şaşkın, Birgül Dikmetaş, and Bekir Erol Ak. 2024. “Determination of the Effects of Drought Stress on Aronia Melanocarpa Cv. Nero in Vitro Conditions”. International Journal of Agriculture Environment and Food Sciences 8 (4): 760-67. https://doi.org/10.31015/jaefs.2024.4.4.
EndNote
Ekinci H, Şaşkın N, Dikmetaş B, Ak BE (December 1, 2024) Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions. International Journal of Agriculture Environment and Food Sciences 8 4 760–767.
IEEE
[1]H. Ekinci, N. Şaşkın, B. Dikmetaş, and B. E. Ak, “Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions”, int. j. agric. environ. food sci., vol. 8, no. 4, pp. 760–767, Dec. 2024, doi: 10.31015/jaefs.2024.4.4.
ISNAD
Ekinci, Heydem - Şaşkın, Necla - Dikmetaş, Birgül - Ak, Bekir Erol. “Determination of the Effects of Drought Stress on Aronia Melanocarpa Cv. Nero in Vitro Conditions”. International Journal of Agriculture Environment and Food Sciences 8/4 (December 1, 2024): 760-767. https://doi.org/10.31015/jaefs.2024.4.4.
JAMA
1.Ekinci H, Şaşkın N, Dikmetaş B, Ak BE. Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions. int. j. agric. environ. food sci. 2024;8:760–767.
MLA
Ekinci, Heydem, et al. “Determination of the Effects of Drought Stress on Aronia Melanocarpa Cv. Nero in Vitro Conditions”. International Journal of Agriculture Environment and Food Sciences, vol. 8, no. 4, Dec. 2024, pp. 760-7, doi:10.31015/jaefs.2024.4.4.
Vancouver
1.Heydem Ekinci, Necla Şaşkın, Birgül Dikmetaş, Bekir Erol Ak. Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions. int. j. agric. environ. food sci. 2024 Dec. 1;8(4):760-7. doi:10.31015/jaefs.2024.4.4