Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS

Murat Güler

doi:10.31015/jaefs.2024.4.11

EN

Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS

Abstract

Phosphate-solubilizing bacteria, which are among the plant growth-promoting bacteria, dissolve insoluble phosphate in the soil by several pathways and promote plant growth. Therefore, it offers an alternative option instead of applying chemical fertilizers that disrupt soil chemistry and ecological balance. Although research on phosphate solubilizing bacteria has increased recently, the research on the peppermint and fennel rhizosphere is still limited. Investigating different rhizospheric local bacteria that can solubilize phosphate and replace chemical fertilizers is necessary. It was determined that 15 of the 53 bacterial isolates obtained from peppermint (Mentha piperita L.) and fennel (Foeniculum vulgare L.) rhizospheres formed a transparent (halo) region around the colonies on Pikovskaya Agar (PKA) medium using the MALDI-TOF MS method. The morphological, biochemical and IAA production of these isolates as well as quantitative measurements of phosphate solubilization by the isolates in NBRIP broth medium was evaluated. The highest efficiency was noted from Bacillus subtilis MMS-7 with solubilization value of 281.6 mg L-1. This was followed by Pseudomonas fluorescens MMS-11 with solubilization value of 263.4 mg L-1 and Bacillus thuringiensis MMS-3 with solubilization value of 172.1 mg L-1, respectively. Among the Phosphate solubilizing bacterial isolates, P solubilization index ranged 1.2-3.7 on PKA agar medium. Additionally, the highest IAA production was noted at 23.38 µg ml-1, using Bacillus subtilis MMS-7. This was followed by Pseudomonas fluorescens MMS-11 with value of 19.72 µg ml-1 and Bacillus thuringiensis using MMS-3 with value of 18.98 µg ml-1. This study demonstrated that selected local isolates can be used as effective phosphate-based microbial fertilizers.

Keywords

Mentha piperita, Foeniculum vulgare, phosphate solubilizing bacteria, MALDI TOF MS

Thanks

Murat Güler thanks Prof. Dr. Khalid Mahmood Khavar for his advice on preparing articles.

References

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Details

Primary Language

English

Subjects

Medicinal and Aromatic Plants , Soil Sciences and Ecology, Soil Microbiology

Journal Section

Research Article

Authors

Murat Güler ^*
0000-0002-3074-6458
Türkiye

Early Pub Date

December 19, 2024

Publication Date

December 28, 2024

Submission Date

September 16, 2024

Acceptance Date

November 10, 2024

Published in Issue

Year 2024 Volume: 8 Number: 4

DOI

https://doi.org/10.31015/jaefs.2024.4.11

IZ

https://izlik.org/JA73WB57PS

APA

Güler, M. (2024). Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS. International Journal of Agriculture Environment and Food Sciences, 8(4), 824-834. https://doi.org/10.31015/jaefs.2024.4.11

AMA

1.Güler M. Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS. int. j. agric. environ. food sci. 2024;8(4):824-834. doi:10.31015/jaefs.2024.4.11

Chicago

Güler, Murat. 2024. “Isolation of Phosphate Solubilizing Bacteria from Different Medicinal Aromatic Plants and Identification Using MALDI TOF MS”. International Journal of Agriculture Environment and Food Sciences 8 (4): 824-34. https://doi.org/10.31015/jaefs.2024.4.11.

EndNote

Güler M (December 1, 2024) Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS. International Journal of Agriculture Environment and Food Sciences 8 4 824–834.

IEEE

[1]M. Güler, “Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS”, int. j. agric. environ. food sci., vol. 8, no. 4, pp. 824–834, Dec. 2024, doi: 10.31015/jaefs.2024.4.11.

ISNAD

Güler, Murat. “Isolation of Phosphate Solubilizing Bacteria from Different Medicinal Aromatic Plants and Identification Using MALDI TOF MS”. International Journal of Agriculture Environment and Food Sciences 8/4 (December 1, 2024): 824-834. https://doi.org/10.31015/jaefs.2024.4.11.

JAMA

1.Güler M. Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS. int. j. agric. environ. food sci. 2024;8:824–834.

MLA

Güler, Murat. “Isolation of Phosphate Solubilizing Bacteria from Different Medicinal Aromatic Plants and Identification Using MALDI TOF MS”. International Journal of Agriculture Environment and Food Sciences, vol. 8, no. 4, Dec. 2024, pp. 824-3, doi:10.31015/jaefs.2024.4.11.

Vancouver

1.Murat Güler. Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS. int. j. agric. environ. food sci. 2024 Dec. 1;8(4):824-3. doi:10.31015/jaefs.2024.4.11

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Abstract

Isolation of phosphate solubilizing bacteria from different medicinal aromatic plants and identification using MALDI TOF MS

Abstract

Keywords

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Effect of flax rhizobacteria on germination and seedling growth under copper toxicity

PGPR-mediated enhancement of growth, phytochemical diversity, and metabolites in black turmeric (Curcuma caesia Roxb)

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